Sensitive detection of α-Fetoprotein using an electrochemiluminescence sensor combining molecular imprinting and aptamer recognition on a graphite carbon nitride/polypyrrole platform
Jie Wang , Mu Yang , Yuxuan Chen , Shouren Li , Yingdan He , Songmei Wu , Yu Yu , Kejian Ding
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引用次数: 0
Abstract
An electrochemiluminescence (ECL) sensor was successfully developed to detect trace amounts of α-fetoprotein (AFP) by combining molecular imprinting (MIP) and nucleic acid aptamer (APT) recognition methods, using graphitic carbon nitride (GCN) and polypyrrole (PPy). The heterojunction material increased the likelihood of more electrons being involved in the ECL detection, improving the sensor’s reliability, stability, and durability. Additionally, the combined techniques strengthened the AFP molecules’ binding to the electrode surface. Under optimal conditions, the sensor had a wide detection range from 10−11 µg/mL to 10−5 µg/mL, with a detection limit (LOD) as low as 10−11 µg/mL (S/N = 3). Tests with real samples confirmed the sensor’s high reliability and feasibility.
期刊介绍：
The Journal of Electroanalytical Chemistry is the foremost international journal devoted to the interdisciplinary subject of electrochemistry in all its aspects, theoretical as well as applied.
Electrochemistry is a wide ranging area that is in a state of continuous evolution. Rather than compiling a long list of topics covered by the Journal, the editors would like to draw particular attention to the key issues of novelty, topicality and quality. Papers should present new and interesting electrochemical science in a way that is accessible to the reader. The presentation and discussion should be at a level that is consistent with the international status of the Journal. Reports describing the application of well-established techniques to problems that are essentially technical will not be accepted. Similarly, papers that report observations but fail to provide adequate interpretation will be rejected by the Editors. Papers dealing with technical electrochemistry should be submitted to other specialist journals unless the authors can show that their work provides substantially new insights into electrochemical processes.
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