Sensitive detection of α-Fetoprotein using an electrochemiluminescence sensor combining molecular imprinting and aptamer recognition on a graphite carbon nitride/polypyrrole platform

IF 4.1 3区化学 Q1 CHEMISTRY, ANALYTICAL Journal of Electroanalytical Chemistry Pub Date : 2025-03-15 Epub Date: 2025-02-10 DOI:10.1016/j.jelechem.2025.119008

Jie Wang , Mu Yang , Yuxuan Chen , Shouren Li , Yingdan He , Songmei Wu , Yu Yu , Kejian Ding

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Abstract

An electrochemiluminescence (ECL) sensor was successfully developed to detect trace amounts of α-fetoprotein (AFP) by combining molecular imprinting (MIP) and nucleic acid aptamer (APT) recognition methods, using graphitic carbon nitride (GCN) and polypyrrole (PPy). The heterojunction material increased the likelihood of more electrons being involved in the ECL detection, improving the sensor’s reliability, stability, and durability. Additionally, the combined techniques strengthened the AFP molecules’ binding to the electrode surface. Under optimal conditions, the sensor had a wide detection range from 10⁻¹¹ µg/mL to 10⁻⁵ µg/mL, with a detection limit (LOD) as low as 10⁻11 µg/mL (S/N = 3). Tests with real samples confirmed the sensor’s high reliability and feasibility.

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结合分子印迹和适配体识别的石墨碳氮/聚吡咯平台电化学发光传感器对α-胎蛋白的灵敏检测

采用氮化石墨碳（GCN）和聚吡咯（PPy）材料，结合分子印迹（MIP）和核酸适体（APT）识别技术，成功研制了一种检测微量α-胎蛋白（AFP）的电化学发光（ECL）传感器。异质结材料增加了更多电子参与ECL检测的可能性，提高了传感器的可靠性、稳定性和耐用性。此外，联合技术增强了AFP分子与电极表面的结合。在最佳条件下，该传感器的检测范围为10 ~ 11µg/mL ~ 10 ~ 5µg/mL，检出限（LOD）低至10 ~ 11µg/mL (S/N = 3)，实际样品的测试证实了该传感器的高可靠性和可行性。

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来源期刊

Journal of Electroanalytical Chemistry 化学-电化学

CiteScore

7.80

自引率

6.70%

发文量

912

审稿时长

2.4 months

期刊介绍： The Journal of Electroanalytical Chemistry is the foremost international journal devoted to the interdisciplinary subject of electrochemistry in all its aspects, theoretical as well as applied. Electrochemistry is a wide ranging area that is in a state of continuous evolution. Rather than compiling a long list of topics covered by the Journal, the editors would like to draw particular attention to the key issues of novelty, topicality and quality. Papers should present new and interesting electrochemical science in a way that is accessible to the reader. The presentation and discussion should be at a level that is consistent with the international status of the Journal. Reports describing the application of well-established techniques to problems that are essentially technical will not be accepted. Similarly, papers that report observations but fail to provide adequate interpretation will be rejected by the Editors. Papers dealing with technical electrochemistry should be submitted to other specialist journals unless the authors can show that their work provides substantially new insights into electrochemical processes.