Jiamin Huang , Hong Zhao , Xinlian Chen, Tianran Lin, Li Hou, Shulin Zhao
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引用次数: 0
Abstract
To meet the increasing demands for highly active and stable nanozymes for bioanalysis, Pt nanoparticles (NPs) were successfully supported on the surface of hydrogen-bonded organic frameworks (Pt/HOFs) to obtain a stable, multifunctional and highly active nanozyme for the colorimetric detection of alkaline phosphatase (ALP). Through a redox reaction, Pt precursors are reduced to ultrasmall Pt NPs that are loaded on HOFs. The Pt/HOFs nanozyme showed excellent oxidase-like, peroxidase-like and some catalase-like activity. To avoid the signal instability caused by H2O2 decomposition, the oxidase-like activity of Pt/HOFs was applied for the detection of ALP using 3,3′, 5,5 ′-tetramethylbenzidine (TMB) as a chromogenic substrate. When the substrate L-ascorbic acid 2-phosphate (AAP) is present, ALP can catalyze AAP to produce strongly reductive ascorbic acid (AA). AA can reduce the oxidation product of TMB. Therefore, a colorimetric sensing strategy for ALP detection was constructed. The linear range of the strategy was from 0.5 to 8 mU mL−1, and the detection limit was 0.46 mU mL−1. Finally, the strategy was successfully applied to the detection of ALP in human serum, which provided a reliable strategy for the colorimetric detection of clinical ALP. This study not only presents a simple approach to maintain the high activity of nanoparticle-based nanozymes, but also expands the application of multifunctional HOF-based materials for future applications.
期刊介绍：
Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science.
The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments.
Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate.
Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to:
Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences,
Novel experimental techniques or instrumentation for molecular spectroscopy,
Novel theoretical and computational methods,
Novel applications in photochemistry and photobiology,
Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.
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