Investigation of the fingerprint-activity relationship of Tremella fuciformis polysaccharides and its mitigating effect on radiation-induced intestinal injury.

Abstract

The aim of this study was to conduct a comprehensive analysis of the relationship between the fingerprint of Tremella fuciformis polysaccharides (TFPs) sourced from China and their bioactivities, with an emphasis on identifying the most bioactive TFP variety that significantly mitigated radiation-induced intestinal injury (RIII). Firstly, the multi-fingerprints we developed indicated that TFPs were classified as acidic, primarily consisting of mannose, rhamnose, glucuronic acid, glucose, xylose, and fucose, with average molecular weight (Mw) ranging from 1.65 × 10³ to 2.50 × 10³ kDa. Subsequently, in vitro activity evaluations demonstrated variability in the antioxidant activities and the inhibitory effects on cancer cell proliferation among TFPs. Multiple linear regression analysis indicated a significant correlation between monosaccharide composition of TFPs and their bioactivity, whereas Mw did not exhibit a similar relationship. Notably, TFP sourced from Zhenjinhui (Gutian, Fujian) (i.e., TFP-2) and Shengkuo (Tongjiang, Sichuan) (i.e., TFP-23) exhibited the most significant bioactivities, both effectively mitigating RIII in mice, with TFP-23 proving to be more effective. Further investigations indicated that TFP-23 provided radioprotective benefits by rectifying RIII-induced dysbiosis of intestinal microbiota and increasing probiotic abundance. Consequently, this study not only clarifies the fingerprint-activity relationship of TFPs but also promotes the potential of TFP-23 as innovative agents for radiation protection.