A Global Comparative Genomic Analysis of Major Bacterial Pathogens in Bovine Mastitis and Lameness.

Abstract

This study presents a comprehensive genomic reanalysis of major bacterial pathogens causing bovine mastitis and lameness, focusing on Staphylococcus aureus, Escherichia coli, Fusobacterium necrophorum, and Treponema phagedenis. Through our analysis of 4326 bacterial genomes from global databases, we identified distinct patterns in genomic diversity, virulence factors and antimicrobial resistance genes across these species. E. coli showed the highest genomic diversity with 3779 isolates, of which 98% exhibited high-quality genome sequences. Similarly, S. aureus demonstrated significant genomic plasticity across 524 isolates, with 99.8% classified as high-quality genomes. Geographical analysis revealed distinct regional variations in strain distribution, with North America contributing 45.3% of all isolates, followed by Asia (21.2%) and Europe (18.1%). Furthermore, we identified novel virulence mechanisms and resistance patterns specific to each pathogen, with particular emphasis on the evolution of antimicrobial resistance genes. Our findings provide crucial insights into pathogen adaptation and host-microbe interactions, suggesting the need for region-specific intervention strategies. These results have significant implications for developing targeted therapeutic approaches and improving bovine health management practices.