Pulsed electromagnetic fields modulate energy metabolism during wound healing process: an in vitro model study.

IF 3.4 2区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE BMC Complementary Medicine and Therapies Pub Date : 2025-02-12 DOI:10.1186/s12906-025-04792-3

Feng Liao, Yamei Li, Zhou Zhang, Qian Yu, Huifang Liu

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Abstract

Background: Pulsed electromagnetic fields (PEMFs) therapy was extensively investigated to treat wound healing, which is a highly metabolically demanding process. However, the effect of PEMFs on energy metabolism in wound healing remains largely unexplored. Therefore, our study aims to demonstrate the role of PEMFs on energy metabolism in wound healing.

Methods: Scratch-wound healing assay and cell viability assay were performed for the in vitro study of the effect of PEMFs on cell migration and viability. Seahorse assay was conducted for energy metabolism analysis while holo-tomographic microscopy for fine changes of L929 cells. Mitochondrial membrane potential assay and intracellular reactive oxygen species (ROS) and pH assay were performed for analyzing the changes of mitochondrial function.

Results: PEMFs with specific parameter (4mT, 80 Hz) promoted cell migration and viability. Glycolysis stress and mitochondria stress test revealed that PEMFs-exposed L929 cells was highly glycolytic for energy generation. Besides, PEMFs enhanced intracellular acidification and maintained low level of intracellular ROS in L929 cells. Compared to control group, much more vesicles were generated and then transported to regions close to the nuclear in L929 cells treated with PEMFs.

Conclusions: Our major findings revealed for the first time that PEMFs induce metabolic reprogramming of fibroblast shifting from mitochondrial respiration to glycolysis, accompanied with an increase of vesicular transport, which is closely related to wound healing in vitro.

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脉冲电磁场调节伤口愈合过程中的能量代谢：体外模型研究。

背景：脉冲电磁场（pemf）疗法被广泛研究用于治疗伤口愈合，这是一个高代谢要求的过程。然而，pemf对伤口愈合中能量代谢的影响在很大程度上仍未被探索。因此，我们的研究旨在证明pemf在伤口愈合中的能量代谢作用。方法：采用刮伤愈合实验和细胞活力实验，研究PEMFs对细胞迁移和活力的影响。海马实验进行能量代谢分析，全息层析显微镜观察L929细胞的细微变化。采用线粒体膜电位测定和细胞内活性氧（ROS）及pH测定分析线粒体功能的变化。结果：特定参数（4mT, 80 Hz）的pemf促进了细胞的迁移和活力。糖酵解应激和线粒体应激测试表明，暴露于pemfs的L929细胞高度糖酵解以产生能量。此外，PEMFs增强了L929细胞的细胞内酸化，维持了低水平的细胞内ROS。与对照组相比，经pemf处理的L929细胞产生了更多的囊泡，然后被运送到靠近核的区域。结论：我们的主要发现首次揭示了PEMFs诱导成纤维细胞从线粒体呼吸转向糖酵解的代谢重编程，并伴有囊泡转运的增加，这与体外伤口愈合密切相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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