Bacillus velezensis RKN1111 enhances resistance against Meloidogyne incognita in Cucumis sativus

IF 3.8 1区农林科学 Q1 AGRONOMY Pest Management Science Pub Date : 2025-02-13 DOI:10.1002/ps.8714

Jianqing Ma, Keran Li, Li He, Ning Wang, Yongpan Xu, Xiaolong Tong, Ruiyan Li, Aimin Zhang, Gangyong Zhao, Dandan Cao

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Abstract

BACKGROUND

Meloidogyne incognita is a highly damaging pathogenic nematode that causes significant annual economic losses. Therefore, the development of reliable biological control agents against M. incognita is imperative. The Bacillus velezensis RKN1111 strain, isolated from inter-root soil, demonstrates the ability to directly kill M. incognita. In this study, we investigated the effect of RKN1111 in inducing resistance to M. incognita in Cucumis sativus and examined changes in the content of immune-responsive substances in the induction-treated cucumber plants.

RESULT

The RKN1111 treatment reduced the number of root galls in infected cucumbers, with a maximum reduction of 78.19%. RKN1111 stably colonized cucumber roots, reaching 3.65 × 10⁶ CFU/g in 3 days. The approach and infestation rates of M. incognita on RKN1111-induced treated cucumber root tips declined at varying time points. Furthermore, RKN1111 induced significant increases (P < 0.05) in hydrogen peroxide (H₂O₂) and superoxide anion (O²⁻) contents, as well as in the callose deposition area in cucumber, by up to 59.84, 83.28, and 61.59%, respectively.

CONCLUSION

RKN1111 has been demonstrated to stably colonize cucumber root systems and defend against M. incognita infestation by inducing systemic resistance in the host plant. Additionally, RKN1111 elevated the levels of immune-responsive substances in cucumber plants. RKN1111 has great potential for application in the integrated pest management of M. incognita. © 2025 Society of Chemical Industry.

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velezensis RKN1111增强黄瓜对嗜甜蛾的抗性。

背景：隐绵线虫是一种破坏性极强的致病性线虫，每年造成重大的经济损失。因此，开发可靠的生物防制剂是势在必行的。从根际土壤中分离的velezensis RKN1111菌株显示了直接杀死M. incognita的能力。在本研究中，我们研究了RKN1111在诱导黄瓜对M. incognita的抗性中的作用，并检测了诱导处理黄瓜植株中免疫应答物质含量的变化。结果：RKN1111处理可减少病黄瓜根瘿数，最大减少率为78.19%。RKN1111在黄瓜根中稳定定植，3 d达到3.65 × 106 CFU/g。不同时间点上，rkn1111诱导的黄瓜根尖上黑穗病螨的接近率和侵染率均呈下降趋势。此外，RKN1111显著提高了黄瓜体内p2o2、超氧阴离子（O2-）含量及胼胝质沉积区O2-含量，分别提高了59.84%、83.28%和61.59%。结论：RKN1111可稳定定殖于黄瓜根系，并通过诱导寄主植物的全身抗性来抵御黑衣m.a cognita侵染。此外，RKN1111提高了黄瓜植株中免疫应答物质的水平。RKN1111在野田鼠有害生物综合治理中具有很大的应用潜力。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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索莱宝

Superoxide Anion Content Detection Kit

索莱宝

Hydrogen Peroxide Content Detection Kit

麦克林

aniline blue

来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.