Toxicity of copper oxide nanoparticles in barley: induction of oxidative stress, hormonal imbalance, and systemic resistances.

Abstract

Background: Over the years, nanoparticles have emerged as a promising approach for improving crop growth, yield, and overall agricultural sustainability. However, there has been growing concern about the potential adverse effects of nanoparticles in the agricultural sector and the environment. The present study aimed to investigate the detrimental effects of high (1000 mg L^-1) concentrations of copper oxide nanoparticles (CuO NPs) on barley seedlings. The equivalent concentrations of CuO bulk and the ionic form of copper were also used in the experiments for comparative analysis. CuO NPs were characterized by Field Emission-Scanning Electron Microscopy, Dynamic Light Scattering, Zeta Potential analysis, and X-ray Diffraction prior to the application. Barley seedlings were subjected to the foliar application of CuO NP, CuO bulk, ionic Cu, and control group. The presence of CuO NPs in barley leaves was confirmed 72 hours after treatment by energy-dispersive X-ray analysis.

Results: The results showed a CuO NPs treatment led to an impairment of nutrient balance in barley leaves. An increase in hydrogen peroxide content followed by the higher specific activity of catalase and ascorbate peroxidase was also observed in response to CuO NPs, CuO bulk, and Cu²⁺ ions. The profile of phytohormones including auxins (IAA and IBA), Gibberellins (GA₁, GA₄, and GA₉), abscisic acid (ABA), ethylene (ET), and jasmonic acid (JA) significantly affected by CuO NPs, CuO bulk, and Cu²⁺ ions. The transcripts of the PR1 gene involved in systemic acquired resistance (SAR) and LOX-1 and PAL involved in induced systemic resistance (ISR) were significantly upregulated in response to CuO NPs treatment.

Conclusion: Our findings suggest that the systemic resistances in barley seedlings were induced by higher accumulation of ABA, ET, and JA under CuO NPs treatment. The activation of systemic resistances indicated the involvement of both SAR and SAR pathways in the response to CuO NPs in barley.