Effect of Photodynamic Therapy With Two Different Photosensitizers on the Viability of Human Dental Pulp Stem Cells.

IF 1.4 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING Journal of lasers in medical sciences Pub Date : 2024-12-28 eCollection Date: 2024-01-01 DOI:10.34172/jlms.2024.70
Shiva Shojaeian, Mohamad Asnaashari, Arash Heidari, Mahsa Sadeghi, Pegah Mehrabinia
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Abstract

Introduction: Photodynamic therapy(PDT)is a minimally invasive technique increasingly used in dentistry for its antimicrobial properties. This research aimed to evaluate the influence of photodynamic therapy (PDT) on the viability of dental pulp stem cells (DPSCs). Methods: In this laboratory-based, experimental study, DPSCs were cultured in Dulbecco's modified Eagle's medium and maintained at 37 °C. The cells were separated into five groups: Toluidine blue (TBO) at concentration of 0.1 mg/mL and 0.5 mg/mL, as well as methylene blue (MB) at concentrations of 0.01 mg/mL and 0.05 mg/mL were added to the wells in groups 1 to 4. The fifth group served as the control group. After 5 minutes of incubation, the experimental groups were irradiated with Fotosan® light-emitting diode (LED) for one minute. Cell viability was assessed after 8, 24, 48, and 72 hours using the methyl thiazolyl tetrazolium (MTT) assay. Results: Time (P<0.000), photosensitizer type/concentration (P<0.0001), and their interaction effect (P<0.000) on cell viability were all significant. Viability in both MB groups was considerably higher than that in the control group at 8 hours (P<0.001). At 24 hours, no significant difference was observed between the experimental groups and the control (P>0.05). At 48 and 72 hours, cell viability in the TBO groups was markedly lower compared to the control group (P<0.01). Conclusion: PDT with MB at the tested concentrations had no adverse effect on DPSCs even in the long- term (48 and 72 hours).

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Journal of lasers in medical sciences
Journal of lasers in medical sciences RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
CiteScore
3.00
自引率
13.30%
发文量
24
期刊介绍: The "Journal of Lasers in Medical Sciences " is a scientific quarterly publication of the Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences. This journal received a scientific and research rank from the national medical publication committee. This Journal accepts original papers, review articles, case reports, brief reports, case series, photo assays, letters to the editor, and commentaries in the field of laser, or light in any fields of medicine such as the following medical specialties: -Dermatology -General and Vascular Surgery -Oncology -Cardiology -Dentistry -Urology -Rehabilitation -Ophthalmology -Otorhinolaryngology -Gynecology & Obstetrics -Internal Medicine -Orthopedics -Neurosurgery -Radiology -Pain Medicine (Algology) -Basic Sciences (Stem cell, Cellular and Molecular application and physic)
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