Milling-based extraction and characterization of Bombyx mori silk glands and fibroin powder for biomaterial use

IF 4.2 2区 工程技术 Q2 ENGINEERING, CHEMICAL Advanced Powder Technology Pub Date : 2025-04-01 Epub Date: 2025-02-16 DOI:10.1016/j.apt.2025.104820
Norihiro Yoshida , Mayumi Tsukada , Masayuki Horio , Akio Nonaka , Akira Nonaka , I. Wuled Lenggoro
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Abstract

This study presents an approach for the extraction of silk glands from Bombyx mori silkworms using a scalable wet milling process, eliminating the need for traditional cocoon processing. We developed a method to harvest silk glands, followed by dry-milling to generate fibroin powders. This technique allows for on-demand extraction by storing silkworms in ethanol aqueous systems or freeze conditions. We systematically investigated the influence of ethanol and freeze-pretreatments on the properties of the extracted silk materials. Ethanol-pretreatment was found to decrease the hardness of silk glands after degumming, while freeze-pretreatment enhanced hardness and introduced a porous structure. Additionally, freeze-pretreated silk powders exhibited larger particle sizes and higher crystallinity compared to those treated with ethanol. These findings highlight the potential of using silk glands as sustainable alternatives to plastics and open new avenues for advanced biomaterial applications.

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生物材料用家蚕丝腺和丝素粉的磨矿提取及特性研究
本研究提出了一种采用可扩展的湿磨工艺提取家蚕蚕丝腺的方法,消除了传统蚕茧加工的需要。我们开发了一种方法来收获丝腺,然后干磨生产丝素粉末。该技术允许在乙醇水系统或冷冻条件下存储蚕按需提取。我们系统地研究了乙醇和冷冻预处理对提取蚕丝材料性能的影响。发现乙醇预处理降低了脱胶后丝腺的硬度,而冷冻预处理提高了丝腺的硬度并引入了多孔结构。此外,冷冻预处理的丝粉比用乙醇处理的丝粉具有更大的粒径和更高的结晶度。这些发现突出了使用丝腺作为塑料可持续替代品的潜力,并为先进生物材料的应用开辟了新的途径。
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来源期刊
Advanced Powder Technology
Advanced Powder Technology 工程技术-工程:化工
CiteScore
9.50
自引率
7.70%
发文量
424
审稿时长
55 days
期刊介绍: The aim of Advanced Powder Technology is to meet the demand for an international journal that integrates all aspects of science and technology research on powder and particulate materials. The journal fulfills this purpose by publishing original research papers, rapid communications, reviews, and translated articles by prominent researchers worldwide. The editorial work of Advanced Powder Technology, which was founded as the International Journal of the Society of Powder Technology, Japan, is now shared by distinguished board members, who operate in a unique framework designed to respond to the increasing global demand for articles on not only powder and particles, but also on various materials produced from them. Advanced Powder Technology covers various areas, but a discussion of powder and particles is required in articles. Topics include: Production of powder and particulate materials in gases and liquids(nanoparticles, fine ceramics, pharmaceuticals, novel functional materials, etc.); Aerosol and colloidal processing; Powder and particle characterization; Dynamics and phenomena; Calculation and simulation (CFD, DEM, Monte Carlo method, population balance, etc.); Measurement and control of powder processes; Particle modification; Comminution; Powder handling and operations (storage, transport, granulation, separation, fluidization, etc.)
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