Magnetically controlled all-in-one sensing platform for triple-mode detection of organophosphorus pesticides using DNA tetrahedrons-polydopamine reporters

IF 10.5 1区生物学 Q1 BIOPHYSICS Biosensors and Bioelectronics Pub Date : 2025-02-12 DOI:10.1016/j.bios.2025.117258

Haiwen Xiao, Jianing Ma, Zihan Liu, Ting Bao, Wei Wen, Xiuhua Zhang, Shengfu Wang

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Abstract

Accurate and on-site detection of organophosphorus pesticides (OPs) in complex matrixes is important for environmental monitoring and food safety. Herein, a colorimetric (CL), photothermal (PT) and fluorescence (FL) triple-mode sensor was constructed for OPs detection based on magnetically controlled all-in-one platform, which was featured of specific recognition unit, abundant loading of DNA tetrahedrons-Fe³⁺-polydopamine (TDN-Fe³⁺-PDA) with peroxidase-like activity, and verifiable triple-mode signal output. Based on the aptamer-target recognition and efficient separation of MBs, large amount of TDN-Fe³⁺-PDA reporters was released. Taking full advantages of the supernatant and precipitation, OPs was sensitively detected with the TDN-Fe³⁺-PDA catalyzed 3,3′,5,5′-tetramethylbenzidine (TMB)-H₂O₂ system (CL mode), photothermal effect of TDN-Fe³⁺-PDA and oxidized TMB (oxTMB) (PT mode), and fluorescent signal of TDN-templated copper nanoclusters on MBs (FL mode). Using profenofos (Pro) as the model target, the triple-mode sensing platform realized sensitive detection of Pro with the detection limits 0.23 ng/mL, 0.40 ng/mL and 0.14 ng/mL for CL, PT and FL mode, respectively. The proposed strategy provided a simple and accurate method for on-site detection of OPs, holding promising application in environmental and food contamination monitoring.

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基于DNA四面体-聚多巴胺报告体的有机磷农药三模检测磁控一体化传感平台

复杂基质中有机磷农药的准确、现场检测对环境监测和食品安全具有重要意义。本文基于磁控一体机平台，构建了OPs检测的比色（CL）、光热（PT）和荧光（FL）三模传感器，该传感器具有特异性识别单元，大量负载具有过氧化物酶样活性的DNA四面体- fe3 +-聚多巴胺（TDN-Fe3+-PDA），具有可验证的三模信号输出。基于适配体-靶标识别和MBs的高效分离，大量的TDN-Fe3+-PDA报道体被发布。利用上清和沉淀的优势，利用TDN-Fe3+-PDA催化的3,3 ',5,5 ' -四甲基联苯胺(TMB)-H2O2体系（CL模式）、TDN-Fe3+-PDA与氧化TMB （oxTMB）的光热效应（PT模式）和TDN-Fe3+-PDA模板铜纳米团簇在MBs上的荧光信号（FL模式）对OPs进行了灵敏检测。以profenofos （Pro）为模型靶标，三模传感平台实现了Pro的灵敏检测，CL、PT和FL模式Pro的检出限分别为0.23 ng/mL、0.40 ng/mL和0.14 ng/mL。该方法为OPs的现场检测提供了一种简便、准确的方法，在环境和食品污染监测中具有广阔的应用前景。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.