Biosensors and Bioelectronics最新文献

Continuous glucose monitoring (CGM) system based on protein hydrogel anti-biofouling coating for long-term accurate and point-of-care glucose monitoring

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-25 DOI: 10.1016/j.bios.2025.117307

Xinglai Tong , Tuohao Jiang , Jiaying Yang , Ying Song , Qi Ao , Jun Tang , Ling Zhang

Continuous Glucose Monitoring (CGM) device was a kind of based on flexible electrode interstitial fluid (ISF) implantation that used electrochemical methods to track blood glucose fluctuations, which made continuous real-time glucose monitoring and personalized blood glucose management increasingly possible. However, when the electrode of CGM in the body fluid environment for a long time, the occurrence of bio-fouling will lead to CGM signal deviation, service life reduction, accuracy decline and other problems. Therefore, in this paper, we constructed a new strategy that provided a well-defined, anti-biofilm coating based and integrated smartphone-controlled wearable microneedle system CGM (acCGM) that can significantly improve accuracy during use and potentially extend service life. In vivo ISF blood glucose monitoring experiment, compared with the commercial blood glucose meter, the acCGM system can accurately monitor the blood glucose level of healthy rats for 21 days. Comparing the two kinds of CGM, it can be found that the MARD of coated CGM within 21 days was 9.69%, and that of uncoated CGM was 16.75%, indicating that the coating had a more obvious anti-biofouling effect. Notably, at 14–21 days after implantation, the MARD of the CGM with the anti-biofouling coating remained at 11.67%, indicating that the acCGM also had the potential to work longer. In addition, the acCGM system with anti-biofouling coating also offered low cost, biosafety, high accuracy and no need for manual calibration.

{"title":"Continuous glucose monitoring (CGM) system based on protein hydrogel anti-biofouling coating for long-term accurate and point-of-care glucose monitoring","authors":"Xinglai Tong , Tuohao Jiang , Jiaying Yang , Ying Song , Qi Ao , Jun Tang , Ling Zhang","doi":"10.1016/j.bios.2025.117307","DOIUrl":"10.1016/j.bios.2025.117307","url":null,"abstract":"<div><div>Continuous Glucose Monitoring (CGM) device was a kind of based on flexible electrode interstitial fluid (ISF) implantation that used electrochemical methods to track blood glucose fluctuations, which made continuous real-time glucose monitoring and personalized blood glucose management increasingly possible. However, when the electrode of CGM in the body fluid environment for a long time, the occurrence of bio-fouling will lead to CGM signal deviation, service life reduction, accuracy decline and other problems. Therefore, in this paper, we constructed a new strategy that provided a well-defined, anti-biofilm coating based and integrated smartphone-controlled wearable microneedle system CGM (acCGM) that can significantly improve accuracy during use and potentially extend service life. In vivo ISF blood glucose monitoring experiment, compared with the commercial blood glucose meter, the acCGM system can accurately monitor the blood glucose level of healthy rats for 21 days. Comparing the two kinds of CGM, it can be found that the MARD of coated CGM within 21 days was 9.69%, and that of uncoated CGM was 16.75%, indicating that the coating had a more obvious anti-biofouling effect. Notably, at 14–21 days after implantation, the MARD of the CGM with the anti-biofouling coating remained at 11.67%, indicating that the acCGM also had the potential to work longer. In addition, the acCGM system with anti-biofouling coating also offered low cost, biosafety, high accuracy and no need for manual calibration.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"277 ","pages":"Article 117307"},"PeriodicalIF":10.7,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-powered wearable electrochemical sensor based on composite conductive hydrogel medium for detection of lactate in human sweat

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-23 DOI: 10.1016/j.bios.2025.117303

Jing Sun , Wanqing Dai , Qiang Guo , Yaru Gao , Jiayu Chen , Jian Lin Chen , Guozhu Mao , Hongyan Sun , Yung-Kang Peng

Sweat, a vital metabolic product in the human body, contains valuable biomarkers that reflect human conditions. Among these, lactate concentration serves as a significant indicator of human physiological states. In this study, we present an innovative self-powered wearable electrochemical sensor designed for real-time lactate detection in human sweat. This sensor utilizes a composite conductive hydrogel medium, showcasing its potential in monitoring and assessing human health. The sensor incorporates two key components: the lactate oxidase/reduced graphene oxide/carbon cloth electrode (LOx/rGO/CCE) as the anode and the bilirubin oxidase/reduced graphene oxide/carbon cloth electrode (BOx/rGO/CCE) as the cathode. These electrodes are integrated into a substrate comprising a conductive hydrogel composed of Poly (3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) and hydrophilic polyurethane (HPU). The sensor's performance was evaluated. The linear detection range spans from 10 nM to 50 mM, with an impressive detection limit of 4.38 nM, demonstrating its high sensitivity and selectivity towards lactate detection with long-term stability. Additionally, this sensor has been successfully applied to real-time monitor lactate concentration on athletes' skin by combining it with self-made equipment and smartphones. The test results demonstrate minimal error compared to the results obtained from high-performance liquid chromatography. This technology opens up a valuable tool for monitoring and assessing human physiological conditions and new possibilities for advancements in health management, sports monitoring, and medical diagnostics.

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引用次数: 0

Real-time cardiomyocyte contraction sensing via a neo-flexible magnetic sensor

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-21 DOI: 10.1016/j.bios.2025.117294

Yue Quan , Sen Ding , Yuxin Wang , Xiuping Chen , Bingpu Zhou , Yinning Zhou

Assessing heart disease and evaluating drug-induced cardiotoxicity require a deep understanding of the contractile properties of cardiac tissue, particularly at the level of individual cardiomyocytes. Traditional methods for measuring cardiomyocyte contractility present several challenges, including limitations in real-time detection, complex and costly sensing platforms, and biocompatibility issues. To address these challenges, we introduce an innovative magnetic sensor that utilizes a flexible coil cantilever, pioneering the application of electromagnetic induction for detecting cardiomyocyte contractility. This marks the first time such technology has been deployed in typical laboratory settings with straightforward configurations. When cardiomyocytes are cultured on these coils and subjected to a static magnetic field, their contractions induce oscillations in the coils, generating an electromotive force that converts mechanical pulsations into electrical signals. This advanced platform enables long-term, real-time monitoring of cardiac functional characteristics, including contractility, beating rate, and rhythm. It also enables the quantitative assessment of cardiovascular dynamics, such as in response to drugs like isoproterenol and verapamil. Offering a uniquely simple, stable, and efficient method for evaluating drug-induced cardiotoxicity, this novel detection platform underscores the transformative potential of flexible magnetic sensors in real-time cellular detection applications.

评估心脏病和药物诱导的心脏毒性需要深入了解心脏组织的收缩特性，特别是单个心肌细胞的收缩特性。测量心肌细胞收缩力的传统方法面临着一些挑战，包括实时检测的局限性、复杂昂贵的传感平台以及生物兼容性问题。为了应对这些挑战，我们推出了一种利用柔性线圈悬臂的创新型磁传感器，开创了应用电磁感应检测心肌细胞收缩力的先河。这标志着此类技术首次在典型的实验室环境中以简单的配置进行部署。当心肌细胞在这些线圈上培养并受到静态磁场作用时，它们的收缩会引起线圈振荡，产生电动势，从而将机械脉动转换为电信号。这种先进的平台可对心脏功能特性（包括收缩力、跳动率和节律）进行长期、实时监测。它还能对心血管动态进行定量评估，例如对异丙肾上腺素和维拉帕米等药物的反应。这种新型检测平台为评估药物引起的心脏毒性提供了一种独特的简单、稳定和高效的方法，凸显了柔性磁传感器在实时细胞检测应用中的变革潜力。

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引用次数: 0

A nanoplasmonic cell-on-a-chip for in situ monitoring of PD-L1+ exosome-mediated immune modulation

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-21 DOI: 10.1016/j.bios.2025.117293

Chuanyu Wang , Lang Zhou , Xuejia Kang , Chung-Hui Huang , Zhuangqiang Gao , Jialiang Shen , Shuai Wu , Siqi Wu , Yuxin Cai , Weiqiang Chen , Siyuan Dai , Pengyu Chen

Despite the transformative impact of immune checkpoint inhibitors (ICIs) targeting the PD-1/PD-L1 pathway in cancer therapy, up to 80% of patients fail to respond, necessitating reliable predictive biomarkers to guide treatment decisions. Recent studies highlight the critical role of tumor-derived exosomal PD-L1 in immune evasion, and its potential as a diagnostic and prognostic biomarker in cancer immunotherapy. However, significant challenges remain in elucidating the functional roles of PD-L1⁺ exosomes in immune suppression, as current methods lack the ability to precisely and simultaneously characterize and monitor exosome secretion and the corresponding immune modulation on site. To address this, we developed an integrated microfluidic platform that combines a digital nanoplasmonic immunoassay with a cell-on-a-chip system, enabling in situ monitoring of PD-L1⁺ exosome secretion and exosome-mediated T cell immune responses. This nanoplasmonic immunoassay integrated cell-on-a-chip (NIIC) creates a localized co-cultured microenvironment that facilitates exosome-mediated cellular interactions without direct contact. The NIIC employs machine-learning assisted signal processing for highly sensitive detection of both exosomes and cytokines, providing spatial and quantitative analysis of immune modulation in situ. Using this system, we demonstrated that PD-L1⁺ exosomes from cancer cells significantly suppressed IFN-γ and IL-2 secretion in neighboring T cells, offering direct insights into exosome-mediated immune suppression. The NIIC platform represents a powerful tool for advancing the understanding of exosome-driven immune modulation and holds potential for predicting clinical responses to anti-PD-1/PD-L1 therapies, paving the way for more personalized cancer immunotherapy strategies.

{"title":"A nanoplasmonic cell-on-a-chip for in situ monitoring of PD-L1+ exosome-mediated immune modulation","authors":"Chuanyu Wang , Lang Zhou , Xuejia Kang , Chung-Hui Huang , Zhuangqiang Gao , Jialiang Shen , Shuai Wu , Siqi Wu , Yuxin Cai , Weiqiang Chen , Siyuan Dai , Pengyu Chen","doi":"10.1016/j.bios.2025.117293","DOIUrl":"10.1016/j.bios.2025.117293","url":null,"abstract":"<div><div>Despite the transformative impact of immune checkpoint inhibitors (ICIs) targeting the PD-1/PD-L1 pathway in cancer therapy, up to 80% of patients fail to respond, necessitating reliable predictive biomarkers to guide treatment decisions. Recent studies highlight the critical role of tumor-derived exosomal PD-L1 in immune evasion, and its potential as a diagnostic and prognostic biomarker in cancer immunotherapy. However, significant challenges remain in elucidating the functional roles of PD-L1<sup>+</sup> exosomes in immune suppression, as current methods lack the ability to precisely and simultaneously characterize and monitor exosome secretion and the corresponding immune modulation on site. To address this, we developed an integrated microfluidic platform that combines a digital nanoplasmonic immunoassay with a cell-on-a-chip system, enabling <em>in situ</em> monitoring of PD-L1<sup>+</sup> exosome secretion and exosome-mediated T cell immune responses. This nanoplasmonic immunoassay integrated cell-on-a-chip (NIIC) creates a localized co-cultured microenvironment that facilitates exosome-mediated cellular interactions without direct contact. The NIIC employs machine-learning assisted signal processing for highly sensitive detection of both exosomes and cytokines, providing spatial and quantitative analysis of immune modulation <em>in situ</em>. Using this system, we demonstrated that PD-L1<sup>+</sup> exosomes from cancer cells significantly suppressed IFN-γ and IL-2 secretion in neighboring T cells, offering direct insights into exosome-mediated immune suppression. The NIIC platform represents a powerful tool for advancing the understanding of exosome-driven immune modulation and holds potential for predicting clinical responses to anti-PD-1/PD-L1 therapies, paving the way for more personalized cancer immunotherapy strategies.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"277 ","pages":"Article 117293"},"PeriodicalIF":10.7,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Touch-based uric acid sweat biosensor towards personal health and nutrition

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-20 DOI: 10.1016/j.bios.2025.117289

Chochanon Moonla , Muhammad Inam Khan , Semra Akgonullu , Tamoghna Saha, Joseph Wang

Monitoring uric acid (UA) levels is critical since elevated UA levels are associated with diverse conditions, such as gout, kidney disorders, kidney stones, hypertension, cardiovascular diseases, and metabolic syndrome. Maintaining balanced UA levels demands reliable and regular monitoring. Traditionally, such frequent UA measurements rely on blood-based UA self-testing strips. Developing sensitive and reliable noninvasive sweat-based UA sensors presents challenges, including the low UA sweat concentrations and interpersonal variations. We present here an attractive on-site UA self-testing approach utilizing a touch-enabled fingertip sweat UA electrochemical biosensor based on a uricase-enzyme electrode and sweat wicking hydrogel. This noninvasive method is rapid, simple, convenient, and painless, leveraging the high sweat rate on the fingertip at rest without any sweat stimulation. The touch-based protocol exhibits a wide linear range of UA concentrations from 10 to 1000 μM, covering normal and elevated UA sweat levels with high selectivity, reproducibility (RSD = 4.94%), good storage stability (1 week), and significant tolerance to temperature and humidity variations. The performance of the UA-touch sweat biosensor was evaluated and validated by parallel blood meter measurements by monitoring dynamically-changing sweat UA levels in healthy subjects after consuming purine-rich meals. The distinct sweat UA temporal profiles among individuals highlight the potential of the touch-based UA biosensor for personal health and nutrition. The speed and simplicity of this sweat UA assay thus encourage frequent self-testing and enhance user's compliance towards dietary interventions and lifestyle changes in connection to diverse healthcare and nutrition applications.

{"title":"Touch-based uric acid sweat biosensor towards personal health and nutrition","authors":"Chochanon Moonla , Muhammad Inam Khan , Semra Akgonullu , Tamoghna Saha, Joseph Wang","doi":"10.1016/j.bios.2025.117289","DOIUrl":"10.1016/j.bios.2025.117289","url":null,"abstract":"<div><div>Monitoring uric acid (UA) levels is critical since elevated UA levels are associated with diverse conditions, such as gout, kidney disorders, kidney stones, hypertension, cardiovascular diseases, and metabolic syndrome. Maintaining balanced UA levels demands reliable and regular monitoring. Traditionally, such frequent UA measurements rely on blood-based UA self-testing strips. Developing sensitive and reliable noninvasive sweat-based UA sensors presents challenges, including the low UA sweat concentrations and interpersonal variations. We present here an attractive on-site UA self-testing approach utilizing a touch-enabled fingertip sweat UA electrochemical biosensor based on a uricase-enzyme electrode and sweat wicking hydrogel. This noninvasive method is rapid, simple, convenient, and painless, leveraging the high sweat rate on the fingertip at rest without any sweat stimulation. The touch-based protocol exhibits a wide linear range of UA concentrations from 10 to 1000 μM, covering normal and elevated UA sweat levels with high selectivity, reproducibility (RSD = 4.94%), good storage stability (1 week), and significant tolerance to temperature and humidity variations. The performance of the UA-touch sweat biosensor was evaluated and validated by parallel blood meter measurements by monitoring dynamically-changing sweat UA levels in healthy subjects after consuming purine-rich meals. The distinct sweat UA temporal profiles among individuals highlight the potential of the touch-based UA biosensor for personal health and nutrition. The speed and simplicity of this sweat UA assay thus encourage frequent self-testing and enhance user's compliance towards dietary interventions and lifestyle changes in connection to diverse healthcare and nutrition applications.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"277 ","pages":"Article 117289"},"PeriodicalIF":10.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143471739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-mode capacitive and localized surface plasmon resonance biosensor based on high-density Au nanoislands

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-20 DOI: 10.1016/j.bios.2025.117274

Jun-Hee Park , Soonil Kwon , Moon-Ju Kim , Zhiquan Song , Hyung Eun Bae , Min-Jung Kang , Jae-Chul Pyun

A capacitive-localized plasmon surface resonance (LSPR) dual-mode biosensor was developed using gold (Au) nanoislands modified on an Au interdigitated electrode (IDE). Au nanoislands were deposited through repeated thermal dewetting to increase their packing density and enhance sensor sensitivity. The response of the capacitive sensor to antibody-antigen interactions was optimized at 0.5 Hz in phosphate-buffered saline. Modification with Au nanoislands significantly reduced the effective electrode gap of the IDE, thereby enhancing the capacitive sensitivity, as evidenced by charge-transfer resistance and electric field analysis. Computer simulations confirmed that the effective electrode gap of a 5 μm gap Au IDE with an 88.1% packing density of Au nanoislands decreased to 525.9 nm. The influence of Au nanoislands on LSPR was assessed through parameters such as λ_max, full width at half maximum, Q factor, and figure of merit. Additionally, the electric field enhancement factor, which indicates LSPR sensitivity, was calculated relative to the packing density of the Au nanoislands. The dual-mode sensor demonstrated efficacy in detecting Salmonella typhimurium, and the capacitive and LSPR sensor results showed a statistically significant correlation.

{"title":"Dual-mode capacitive and localized surface plasmon resonance biosensor based on high-density Au nanoislands","authors":"Jun-Hee Park , Soonil Kwon , Moon-Ju Kim , Zhiquan Song , Hyung Eun Bae , Min-Jung Kang , Jae-Chul Pyun","doi":"10.1016/j.bios.2025.117274","DOIUrl":"10.1016/j.bios.2025.117274","url":null,"abstract":"<div><div>A capacitive-localized plasmon surface resonance (LSPR) dual-mode biosensor was developed using gold (Au) nanoislands modified on an Au interdigitated electrode (IDE). Au nanoislands were deposited through repeated thermal dewetting to increase their packing density and enhance sensor sensitivity. The response of the capacitive sensor to antibody-antigen interactions was optimized at 0.5 Hz in phosphate-buffered saline. Modification with Au nanoislands significantly reduced the effective electrode gap of the IDE, thereby enhancing the capacitive sensitivity, as evidenced by charge-transfer resistance and electric field analysis. Computer simulations confirmed that the effective electrode gap of a 5 μm gap Au IDE with an 88.1% packing density of Au nanoislands decreased to 525.9 nm. The influence of Au nanoislands on LSPR was assessed through parameters such as <em>λ<sub>max</sub></em>, full width at half maximum, Q factor, and figure of merit. Additionally, the electric field enhancement factor, which indicates LSPR sensitivity, was calculated relative to the packing density of the Au nanoislands. The dual-mode sensor demonstrated efficacy in detecting Salmonella typhimurium, and the capacitive and LSPR sensor results showed a statistically significant correlation.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"277 ","pages":"Article 117274"},"PeriodicalIF":10.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143464219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paper-based degradable, label-free microRNA sensing platform based on oxide thin-film transistor arrays

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-20 DOI: 10.1016/j.bios.2025.117291

Xuemei Yin , Qindong Guo , Xingqi Ji , Xiaoqian Li , Hao Xue , Qian Xin , Jiawei Zhang , Zhuocheng Yan , Aimin Song

MicroRNAs have started being used as an effective marker in early diagnosis and preoperative monitoring of cancers in recent years. Traditional microRNA testing technology, such as quantitative reverse transcription polymerase chain reaction (qRT-PCR) and various fluorescent and colorimetric assays, often suffer from complicated operations and limited sensitivity. They also produce significant electronic waste posing threats to human health and environment. Here, we propose a degradable biosensor using indium-gallium-zinc oxide (IGZO) thin-film transistor (TFT) arrays for rapid and highly sensitive detection of microRNAs from glioma exosome extracts (g-miRNAs). The IGZO transistor arrays fabricated on nanofibrillated cellulose paper exhibit high electronic performance with a current on/off ratio >1.5 × 10⁶, a mobility of 9.6 cm² V⁻¹·s⁻¹, a subthreshold swing <0.5 V·dec⁻¹, and excellent bias stress stability. Specific DNA probes in the IGZO channel bind selectively with g-miRNA targets to form DNA-RNA double strands, offering high specificity even when coexisting with high concentrations of nonspecific microRNAs. The inherent negative charges in DNA and g-miRNA molecules sensitively modulate the IGZO channel conductivity, leading to a positive shift of threshold voltage and decrease of source-drain current. These changes are linearly correlated with microRNA concentrations from 1 fM to 100 pM, with a detection limitation of 350 aM. Furthermore, the paper-based IGZO transistor array nearly completely dissolves in a NaOH solution after 300 min. The proposed approach combines easy-to-operate, point-of-care microRNA testing with lightweight, low-cost, biocompatible, and degradable devices, showing great promise for early diagnosis of glioma and most likely also other tumors.

{"title":"Paper-based degradable, label-free microRNA sensing platform based on oxide thin-film transistor arrays","authors":"Xuemei Yin , Qindong Guo , Xingqi Ji , Xiaoqian Li , Hao Xue , Qian Xin , Jiawei Zhang , Zhuocheng Yan , Aimin Song","doi":"10.1016/j.bios.2025.117291","DOIUrl":"10.1016/j.bios.2025.117291","url":null,"abstract":"<div><div>MicroRNAs have started being used as an effective marker in early diagnosis and preoperative monitoring of cancers in recent years. Traditional microRNA testing technology, such as quantitative reverse transcription polymerase chain reaction (qRT-PCR) and various fluorescent and colorimetric assays, often suffer from complicated operations and limited sensitivity. They also produce significant electronic waste posing threats to human health and environment. Here, we propose a degradable biosensor using indium-gallium-zinc oxide (IGZO) thin-film transistor (TFT) arrays for rapid and highly sensitive detection of microRNAs from glioma exosome extracts (g-miRNAs). The IGZO transistor arrays fabricated on nanofibrillated cellulose paper exhibit high electronic performance with a current on/off ratio >1.5 × 10<sup>6</sup>, a mobility of 9.6 cm<sup>2</sup> V<sup>−1</sup>·s<sup>−1</sup>, a subthreshold swing <0.5 V·dec<sup>−1</sup>, and excellent bias stress stability. Specific DNA probes in the IGZO channel bind selectively with g-miRNA targets to form DNA-RNA double strands, offering high specificity even when coexisting with high concentrations of nonspecific microRNAs. The inherent negative charges in DNA and g-miRNA molecules sensitively modulate the IGZO channel conductivity, leading to a positive shift of threshold voltage and decrease of source-drain current. These changes are linearly correlated with microRNA concentrations from 1 fM to 100 pM, with a detection limitation of 350 aM. Furthermore, the paper-based IGZO transistor array nearly completely dissolves in a NaOH solution after 300 min. The proposed approach combines easy-to-operate, point-of-care microRNA testing with lightweight, low-cost, biocompatible, and degradable devices, showing great promise for early diagnosis of glioma and most likely also other tumors.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"277 ","pages":"Article 117291"},"PeriodicalIF":10.7,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143479802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Titanium nitride meta-biosensors targeting extracellular vesicles for high-sensitivity prostate cancer detection

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-19 DOI: 10.1016/j.bios.2025.117288

Fajun Li , Yuanyuan Jiang , Xuegang Wang , Yuan Gao , Ching Shu Lo , Shengdong Su , Zhilin Wu , Bingliang Jiang , Zhongjie Zhao , Shaowei Lin , Yinong Xie , Junjie Chen , Qiwei Guo , Zhaogang Dong , Jinfeng Zhu

Disposable plasmonic metasurfaces with high biosensing performance are urgently sought for clinical label-free detection. Low-cost aluminum (Al) and titanium nitride (TiN) offer promising alternatives to noble metals for constructing these metasurfaces. However, Al suffers from limited chemical stability, and TiN exhibits weak plasmonic effects, both of which hinder their application in meta-biosensing. Here we integrate their complementary advantages and propose the TiN/Al meta-biosensors. They not only empower the unique near-field enhancement for sensing by TiN/Al hybrid plasmonic modes, but also construct a robust TiN armor against external wear, heat, moisture and corrosion during the bio-detection process. Compared to traditional gold-based counterparts, our meta-biosensors offer superior optical sensitivity at a much lower cost and with fewer pretreatment steps. The excellent biosensing performance facilitates the development of a high-throughput detection system for serum small extracellular vesicles (sEVs), aiding in the diagnosis and follow-up of prostate cancer. The sEVs meta-biosensing demonstrates a diagnostic sensitivity of 100% for significantly distinguishing early cancer, breaking through the conventional testing limitation. Moreover, it doubles the prediction accuracy of cancer recurrence risk following surgery. Our research highlights the potential for large-scale development of powerful meta-biosensors based on non-noble materials, opening up significant opportunities in cancer diagnosis and prognosis.

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引用次数: 0

A “turn-on” intracellular pH probe for the quantitative monitoring of lysosomal alkalization in living cells

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-19 DOI: 10.1016/j.bios.2025.117285

Hejian Ke , Jiaqi Yang , Wanping Zhang , Pengli Yang , Yiwu Wang , Ningrong Wang , Jiahao Bai , Huaiyi Yin , Yanyan Chen , Xinhong Chen , Peishan Fu , Yongjun Gan , Guangchao Zang , Qian Liu

A slight elevation in lysosomal pH can lead to indigestion or nonspecific hydrolysis, thereby increasing the risk of various neurodegenerative diseases and cancer. Therefore, accurate monitoring of lysosomal pH changes in living cells is essential for the diagnosis and treatment of such diseases, despite the significant challenges involved. In this study, we synthesized a pH-dependent fluorescent probe, B26, which comprises 1,8-naphthalimide as the fluorescent chromophore, an N-(2-hydroxyethyl) piperazine group for lysosome targeting, and a hydroxyethyl group to increase solubility and regulate pKa. B26 demonstrated high sensitivity, selectivity, and reversibility in response to H⁺, and exhibited a remarkable 98-fold increase in fluorescence intensity between pH 2.0 and pH 11.0, with a pKa value of 7.0, highlighting its “turn-on” fluorescence property. Density functional theory calculations and ¹H NMR titration revealed that the pH-sensing mechanism of B26 relies on the inhibition of photoinduced electron transfer from the N-(2-hydroxyethyl) piperazine group to the naphthalimide moiety under acidic conditions. Importantly, B26 effectively labeled lysosomes and displayed significant sensitivity to pH changes, facilitating the quantitative detection of pH shifts during lysosomal alkalization in living cells due to its elevated pKa. These findings suggest that B26 successfully addresses the limitations of existing lysosomal pH probes, particularly in detecting pH changes within the near-neutral range. Furthermore, both the zebrafish model and subcutaneous imaging support the application of B26 in in vivo settings. Given its exceptional properties, B26 holds enormous potential for the research and diagnosis of pH-related diseases.

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引用次数: 0

Colorimetric – Fluorescence – Photothermal tri-mode sensor array combining the machine learning method for the selective identification of sulfonylurea pesticides

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-02-19 DOI: 10.1016/j.bios.2025.117286

Tian Tian , Donghui Song , Linxue Zhen , Zhichun Bi , Ling Zhang , Hui Huang , Yongxin Li

Though cholinesterase-based method could detect two types of pesticides (organophosphorus and carbamate), they had weak sensing on sulfonylurea pesticides. In our previous work, the peroxidase-like reaction system of nanozyme – H₂O₂ – TMB showed selective detection of sulfonylurea pesticides, but the single-signal output sensing platform was easily affected by complex matrix background, cross-contamination and human error. Therefore, this work used colorimetric, photothermal, and fluorescent signals of the nanozyme reaction as sensing units for the detection of pesticides. This is the first time that photothermal signals have been used to construct a sensor array. When the concentration of interfering substances was 25 times that of pesticides, the method was still unaffected and had excellent selectivity and anti-interference performance. Meanwhile, a concentration-independent differentiation mode was established based on the K-nearest neighbor (KNN) algorithm. The pesticides were detected and distinguished with 100% accuracy. This work contributed to the detection of sulfonylurea pesticides in complex environmental/food matrices, bridging the gap of existing pesticide detection methods and providing an effective method for food safety detection.

{"title":"Colorimetric – Fluorescence – Photothermal tri-mode sensor array combining the machine learning method for the selective identification of sulfonylurea pesticides","authors":"Tian Tian , Donghui Song , Linxue Zhen , Zhichun Bi , Ling Zhang , Hui Huang , Yongxin Li","doi":"10.1016/j.bios.2025.117286","DOIUrl":"10.1016/j.bios.2025.117286","url":null,"abstract":"<div><div>Though cholinesterase-based method could detect two types of pesticides (organophosphorus and carbamate), they had weak sensing on sulfonylurea pesticides. In our previous work, the peroxidase-like reaction system of nanozyme – H<sub>2</sub>O<sub>2</sub> – TMB showed selective detection of sulfonylurea pesticides, but the single-signal output sensing platform was easily affected by complex matrix background, cross-contamination and human error. Therefore, this work used colorimetric, photothermal, and fluorescent signals of the nanozyme reaction as sensing units for the detection of pesticides. This is the first time that photothermal signals have been used to construct a sensor array. When the concentration of interfering substances was 25 times that of pesticides, the method was still unaffected and had excellent selectivity and anti-interference performance. Meanwhile, a concentration-independent differentiation mode was established based on the K-nearest neighbor (KNN) algorithm. The pesticides were detected and distinguished with 100% accuracy. This work contributed to the detection of sulfonylurea pesticides in complex environmental/food matrices, bridging the gap of existing pesticide detection methods and providing an effective method for food safety detection.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"277 ","pages":"Article 117286"},"PeriodicalIF":10.7,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0