Biosensors and Bioelectronics最新文献

英文中文

Portable electrophoretic lateral flow biosensing for ultra-sensitive human lactate dehydrogenase detection in serum samples

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-04-22 DOI: 10.1016/j.bios.2025.117504

Amadeo Sena-Torralba , Yulieth D. Banguera-Ordoñez , Javier Carrascosa , Ángel Maquieira , Sergi Morais

Lateral flow immunoassays are globally recognized for their simplicity, cost-effectiveness, and rapid qualitative and semiquantitative analyses, making them indispensable as point-of-care screening tools. However, their limited sensitivity restricts their application in clinical settings, requiring the detection of ultralow analyte concentrations in complex sample matrices. To address these challenges, we present a portable biosensing platform integrating battery-powered electrokinetic-driven microfluidics to enhance sensitivity while preserving point-of-care functionality. Our lightweight (151 g), 3D-printed electrophoretic device (€82) supports the simultaneous analysis of three samples and operates with an ultra-low power consumption of 225 mAh⁻¹, enabling 44 h of operation on a single charge. By optimizing key parameters such as Joule heating, buffer evaporation, and electroosmotic flow, the device enables iterative incubation and washing steps directly on the nitrocellulose strip, capabilities unattainable with conventional capillarity-driven LFIAs. This advanced biosensing platform achieves a detection limit of 70 pg mL⁻¹ for human lactate dehydrogenase (h-LDH), a key cancer biomarker, using gold nanoparticles as signal transducers. This result means a 367-fold improvement in sensitivity. Offering rapid, cost-effective, and ultra-sensitive biomarker quantification, this approach holds significant promise for transforming precision medicine, particularly in monitoring LDH-related cancer therapies.

{"title":"Portable electrophoretic lateral flow biosensing for ultra-sensitive human lactate dehydrogenase detection in serum samples","authors":"Amadeo Sena-Torralba , Yulieth D. Banguera-Ordoñez , Javier Carrascosa , Ángel Maquieira , Sergi Morais","doi":"10.1016/j.bios.2025.117504","DOIUrl":"10.1016/j.bios.2025.117504","url":null,"abstract":"<div><div>Lateral flow immunoassays are globally recognized for their simplicity, cost-effectiveness, and rapid qualitative and semiquantitative analyses, making them indispensable as point-of-care screening tools. However, their limited sensitivity restricts their application in clinical settings, requiring the detection of ultralow analyte concentrations in complex sample matrices. To address these challenges, we present a portable biosensing platform integrating battery-powered electrokinetic-driven microfluidics to enhance sensitivity while preserving point-of-care functionality. Our lightweight (151 g), 3D-printed electrophoretic device (€82) supports the simultaneous analysis of three samples and operates with an ultra-low power consumption of 225 mAh<sup>−1</sup>, enabling 44 h of operation on a single charge. By optimizing key parameters such as Joule heating, buffer evaporation, and electroosmotic flow, the device enables iterative incubation and washing steps directly on the nitrocellulose strip, capabilities unattainable with conventional capillarity-driven LFIAs. This advanced biosensing platform achieves a detection limit of 70 pg mL<sup>−1</sup> for human lactate dehydrogenase (h-LDH), a key cancer biomarker, using gold nanoparticles as signal transducers. This result means a 367-fold improvement in sensitivity. Offering rapid, cost-effective, and ultra-sensitive biomarker quantification, this approach holds significant promise for transforming precision medicine, particularly in monitoring LDH-related cancer therapies.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117504"},"PeriodicalIF":10.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterojunction nanofluidic memristors based on peptide chain valves for neuromorphic applications

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-04-18 DOI: 10.1016/j.bios.2025.117496

Honglin Lv , Yin Zhang

Memristors exhibit significant potential for neuromorphic computing due to their unique properties. This study introduces a heterojunction nanofluidic memristor (HJNFM) and explores its applications in simulating synapses and constructing neural networks. The HJNFM consists of a SnS₂ and MoS₂ heterojunction nanochannel with a peptide chain valve. The opening and closing dynamics of peptide chain valve alter ionic conductance of the nanochannel and realize the memristor characteristics. The sequence of the peptide chain also affects the electrical properties of HJNFM. Additionally, by setting up multi SnS₂ strips in the nanochannel, the multi-HJNFM can achieve permanent memory and emulate synaptic features including short-term and long-term memory. Notably, we construct a convolutional neural network from multi-HJNFMs, which achieves 94 % accuracy in a digit recognition task. This study presents a new approach to constructing nanofluidic memristors, which could be advantageous for developing new forms of neuromorphic computing in the future.

引用次数: 0

crRNA array-mediated CRISPR/Cas12a coupling with dual RPA for highly sensitive detection of Streptomyces aureofaciens Tü117 from hypertension with multi-signal output

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-04-16 DOI: 10.1016/j.bios.2025.117493

Tingting Zhou , Gongle Shen , Linling Zhong , Genchang Chen , Liyuan Meng , Wenyin He , Jing Liu , Sen Yang , Yang Luo , Xianfeng Wang

Accurate and sensitive detection of Streptomyces aureofaciens Tü117 is crucial for hypertension classification and early warning. To achieve this, a dual recombinase polymerase amplification coupled with a crRNA array-mediated CRISPR/Cas12a assay (DR-CAMCas) was developed, enabling multi-signal output for precise identification and detection of S. aureofaciens Tü117. The 16S rDNA and LipReg4 genes of S. aureofaciens Tü117 are amplified simultaneously via one-step dual RPA, activating the crRNA array-mediated CRISPR/Cas12a system to cleave exogenous FQ-reporters, releasing fluorescent signals. DR-CAMCas offers high amplification efficiency, multi-site recognition through crRNA array signal superposition, and the programmability of CRISPR/Cas12a, achieving ultrasensitive detection with a linear range of 10 to 10⁸ cfu/mL and a limit of detection of approximately 3 cfu/mL. DR-CAMCas successfully detected S. aureofaciens Tü117 in fecal samples from high-salt diet-induced hypertensive mice and hypertensive patients, matching qPCR results and demonstrating high reliability and practicality. Additionally, target-induced cleavage of a DNA linker by DR-CAMCas dispersed AuNPs-DNA probes, enabling colorimetric detection. Integrated onto lateral flow sensors, DR-CAMCas allows point-of-care testing via simple visual strip analysis. Its triple signal output meets diverse detection needs, offering a promising tool for diagnosing salt-sensitive hypertension.

{"title":"crRNA array-mediated CRISPR/Cas12a coupling with dual RPA for highly sensitive detection of Streptomyces aureofaciens Tü117 from hypertension with multi-signal output","authors":"Tingting Zhou , Gongle Shen , Linling Zhong , Genchang Chen , Liyuan Meng , Wenyin He , Jing Liu , Sen Yang , Yang Luo , Xianfeng Wang","doi":"10.1016/j.bios.2025.117493","DOIUrl":"10.1016/j.bios.2025.117493","url":null,"abstract":"<div><div>Accurate and sensitive detection of <em>Streptomyces aureofaciens</em> Tü117 is crucial for hypertension classification and early warning. To achieve this, a dual recombinase polymerase amplification coupled with a crRNA array-mediated CRISPR/Cas12a assay (DR-CAMCas) was developed, enabling multi-signal output for precise identification and detection of <em>S. aureofaciens</em> Tü117. The 16S rDNA and LipReg4 genes of <em>S. aureofaciens</em> Tü117 are amplified simultaneously via one-step dual RPA, activating the crRNA array-mediated CRISPR/Cas12a system to cleave exogenous FQ-reporters, releasing fluorescent signals. DR-CAMCas offers high amplification efficiency, multi-site recognition through crRNA array signal superposition, and the programmability of CRISPR/Cas12a, achieving ultrasensitive detection with a linear range of 10 to 10<sup>8</sup> cfu/mL and a limit of detection of approximately 3 cfu/mL. DR-CAMCas successfully detected <em>S. aureofaciens</em> Tü117 in fecal samples from high-salt diet-induced hypertensive mice and hypertensive patients, matching qPCR results and demonstrating high reliability and practicality. Additionally, target-induced cleavage of a DNA linker by DR-CAMCas dispersed AuNPs-DNA probes, enabling colorimetric detection. Integrated onto lateral flow sensors, DR-CAMCas allows point-of-care testing via simple visual strip analysis. Its triple signal output meets diverse detection needs, offering a promising tool for diagnosing salt-sensitive hypertension.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117493"},"PeriodicalIF":10.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-channel fluorescent probe for simultaneously visualizing ONOO− and viscosity in epilepsy, non-alcoholic fatty liver and tumoral ferroptosis models

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-04-16 DOI: 10.1016/j.bios.2025.117495

Qi Zan , Li Fan , Rui Wang , Hui Wang , Yue Huang , Xue Yu , Yuewei Zhang , Chuan Dong , Shaomin Shuang

Intracellular peroxynitrite anion (ONOO⁻) and viscosity play a major part in sustaining redox homeostasis, modulating substance transport and signal transduction. Abnormalities in these factors are closely associated with multiple physiological and pathological processes. Nevertheless, due to the absence of appropriate multifunctional fluorescent sensors, concurrent identification of ONOO⁻ and viscosity has not been achieved in many diseases, such as epilepsy and tumoral ferroptosis models. Herein, a new near-infrared (NIR) fluorescent probe (QX-DP) was rationally conceived for concurrent detection of ONOO⁻ and viscosity. QX-DP was highly sensitive to viscosity at 668 nm and ONOO⁻ at 752 nm which exhibited significant “turn-on” fluorescence signals, respectively. Making use of the QX-DP with dual-channel imaging capability, the ONOO⁻ and viscosity elevated levels in brain tissue of epileptic mice were revealed for the first time, and the visualization diagnosis of non-alcoholic liver injury (NAFL) disease model was achieved. Most importantly, the concurrent utilization of viscosity and ONOO⁻ for visualizing tumor ferroptosis has been successfully achieved not only in cancer cells and zebrafish but also in tumor mice models. Undoubtedly, in comparison with detection of a single biomarker, monitoring dual biomarkers at the same time may offer a more sensitive and dependable strategy in the diagnosis and image-assisted surgery of oxidative stress and viscosity related diseases.

{"title":"Dual-channel fluorescent probe for simultaneously visualizing ONOO− and viscosity in epilepsy, non-alcoholic fatty liver and tumoral ferroptosis models","authors":"Qi Zan , Li Fan , Rui Wang , Hui Wang , Yue Huang , Xue Yu , Yuewei Zhang , Chuan Dong , Shaomin Shuang","doi":"10.1016/j.bios.2025.117495","DOIUrl":"10.1016/j.bios.2025.117495","url":null,"abstract":"<div><div>Intracellular peroxynitrite anion (ONOO<sup>−</sup>) and viscosity play a major part in sustaining redox homeostasis, modulating substance transport and signal transduction. Abnormalities in these factors are closely associated with multiple physiological and pathological processes. Nevertheless, due to the absence of appropriate multifunctional fluorescent sensors, concurrent identification of ONOO<sup>−</sup> and viscosity has not been achieved in many diseases, such as epilepsy and tumoral ferroptosis models. Herein, a new near-infrared (NIR) fluorescent probe (<strong>QX-DP</strong>) was rationally conceived for concurrent detection of ONOO<sup>−</sup> and viscosity. <strong>QX-DP</strong> was highly sensitive to viscosity at 668 nm and ONOO<sup>−</sup> at 752 nm which exhibited significant “turn-on” fluorescence signals, respectively. Making use of the <strong>QX-DP</strong> with dual-channel imaging capability, the ONOO<sup>−</sup> and viscosity elevated levels in brain tissue of epileptic mice were revealed for the first time, and the visualization diagnosis of non-alcoholic liver injury (NAFL) disease model was achieved. Most importantly, the concurrent utilization of viscosity and ONOO<sup>−</sup> for visualizing tumor ferroptosis has been successfully achieved not only in cancer cells and zebrafish but also in tumor mice models. Undoubtedly, in comparison with detection of a single biomarker, monitoring dual biomarkers at the same time may offer a more sensitive and dependable strategy in the diagnosis and image-assisted surgery of oxidative stress and viscosity related diseases.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117495"},"PeriodicalIF":10.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational development of Nile red derivatives with significantly improved specificity and photostability for advanced fluorescence imaging of lipid droplets

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-04-16 DOI: 10.1016/j.bios.2025.117494

Huanlong Zheng , Hao Sha , Ri Zhou , Yu Wu , Chenguang Wang , Shangguo Hou , Geyu Lu

Since the first report of Nile Red as a fluorescent probe for lipid droplets (LDs) imaging was published in 1985, this fluorescent probe has been widely used for nearly 40 years, and so far, it is still one of the most commonly used probes for LDs imaging. Although Nile Red has achieved continuous success, it has gradually emerged two major limitations (poor LDs specificity and low photostability) which directly limit the study of LDs via advanced fluorescence imaging techniques. In this context, we have developed a new synthetic route to conveniently prepare a series of Nile Red derivatives (NR-1 to NR-15). With these 15 derivatives in hand, the relationships between molecular structures and their properties (LDs specificity, photostability) have been comprehensively investigated. Consequently, we have rationally designed a new Nile Red derivative, NR-11, which exhibits significantly improved LDs specificity and photostability. Utilizing this new LDs probe, we have successfully conducted various advanced fluorescence imaging, e.g. time-lapse three-dimensional (3D) confocal imaging of cells, time-lapse 3D dynamic tracking of a single LD, and two-photon 3D imaging of tissues. These advanced imaging results not only demonstrate the utility of this new fluorescent probe but also provide novel insights into the cell biology study of LDs.

{"title":"Rational development of Nile red derivatives with significantly improved specificity and photostability for advanced fluorescence imaging of lipid droplets","authors":"Huanlong Zheng , Hao Sha , Ri Zhou , Yu Wu , Chenguang Wang , Shangguo Hou , Geyu Lu","doi":"10.1016/j.bios.2025.117494","DOIUrl":"10.1016/j.bios.2025.117494","url":null,"abstract":"<div><div>Since the first report of Nile Red as a fluorescent probe for lipid droplets (LDs) imaging was published in 1985, this fluorescent probe has been widely used for nearly 40 years, and so far, it is still one of the most commonly used probes for LDs imaging. Although Nile Red has achieved continuous success, it has gradually emerged two major limitations (poor LDs specificity and low photostability) which directly limit the study of LDs via advanced fluorescence imaging techniques. In this context, we have developed a new synthetic route to conveniently prepare a series of Nile Red derivatives (NR-1 to NR-15). With these 15 derivatives in hand, the relationships between molecular structures and their properties (LDs specificity, photostability) have been comprehensively investigated. Consequently, we have rationally designed a new Nile Red derivative, NR-11, which exhibits significantly improved LDs specificity and photostability. Utilizing this new LDs probe, we have successfully conducted various advanced fluorescence imaging, <em>e.g.</em> time-lapse three-dimensional (3D) confocal imaging of cells, time-lapse 3D dynamic tracking of a single LD, and two-photon 3D imaging of tissues. These advanced imaging results not only demonstrate the utility of this new fluorescent probe but also provide novel insights into the cell biology study of LDs.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117494"},"PeriodicalIF":10.7,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A self-catalytic UCNP-based nanomachine activated by duplex DNA for highly sensitive detection of CTCs

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-04-16 DOI: 10.1016/j.bios.2025.117483

Kemeng Zhang , Jiajun Li , Ye Li , Wen Zhang , Guohai Liang , Tao Zhang

Detection of circulating tumor cells (CTCs) has proven to be a crucial approach for early diagnosis, prognosis, and monitoring of cancer treatment. However, due to the low abundance of CTCs in blood, achieving accurate detection in the presence of a large number of blood cells remains challenging. In this study, we present a novel self-catalytic nanomachine for quantitative detection of CTCs, which includes a dual aptamer-triggered Catalytic Hairpin Assembly (CHA) reaction and subsequent UCNP-DNA-based biosensing. The dual-aptamer recognition, the two-step CHA reaction and the UCNP ratiometric sensing luminescence provide the assay with high specificity and sensitivity. Using MDA-MB-231 cells as model targets, the proposed detection system affords a wide linear detection range and a detection limit as low as 3 cells. Our system offers sensitive detection of CTCs without the need for enzymatic involvement, indicating its substantial potential for early cancer diagnosis and treatment based on CTCs.

引用次数: 0

A dual-Color closed bipolar electrochemiluminescence platform for visual simultaneous diagnosis of two pluripotency markers in cancer patients’ urine

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-04-15 DOI: 10.1016/j.bios.2025.117482

Nastaran Arab , Morteza Hosseini , Guobao Xu , Niloufar Sadeghi , Hodjattallah Rabbani

Here, for the first time, we introduce a simple yet effective one-step visual approach for the concurrent detection of octamer-binding transcription factor 4 (Oct-4) and Nanog, two pivotal homeobox genes involved in regulating pluripotency in both stem cells and certain cancer cells, within human urine samples. We utilized a closed bipolar electrochemistry system to enable the electrochemiluminescence (ECL) detection of pluripotency biomarkers. This was accomplished by capturing the ECL of Ru(bpy)₃²⁺ loaded into mesoporous SiO₂ nanoparticles and luminol incorporated into luminol@MIL-53 on the upper and lower anodic poles of BPEs in separate chambers of a bipolar cell using a digital camera. An identical electric potential was applied across the bipolar electrodes, driving the reduction of thionine acetate at the cathodic poles and thereby facilitating simultaneous light emission of the luminophores. Moreover, the incorporation of electroactive Ti₃C₂T_x MXene-TiO₂ nanosheets catalyzed the electro-oxidation of co-reactants within both the Ru(bpy)₃²⁺/TPrA and luminol/H₂O₂ systems, thereby enhancing the signal-to-background ratio and eliciting a significantly amplified visual ECL response. Under optimized conditions, the immunosensor displayed a linear response across a broad range of 100 pg mL⁻¹ to 400 ng/mL for Nanog, with a detection limit of 21.58 pg mL⁻¹, and a linear response from 200 pg mL⁻¹ to 400 ng/mL with a detection limit of 106.24 pg mL⁻¹ for Oct-4. This study supports the immunosensor's potential for broader clinical application, where its high specificity and sensitivity could offer significant benefits in cancer diagnostics and stem cell research.

{"title":"A dual-Color closed bipolar electrochemiluminescence platform for visual simultaneous diagnosis of two pluripotency markers in cancer patients’ urine","authors":"Nastaran Arab , Morteza Hosseini , Guobao Xu , Niloufar Sadeghi , Hodjattallah Rabbani","doi":"10.1016/j.bios.2025.117482","DOIUrl":"10.1016/j.bios.2025.117482","url":null,"abstract":"<div><div>Here, for the first time, we introduce a simple yet effective one-step visual approach for the concurrent detection of octamer-binding transcription factor 4 (Oct-4) and Nanog, two pivotal homeobox genes involved in regulating pluripotency in both stem cells and certain cancer cells, within human urine samples. We utilized a closed bipolar electrochemistry system to enable the electrochemiluminescence (ECL) detection of pluripotency biomarkers. This was accomplished by capturing the ECL of Ru(bpy)<sub>3</sub><sup>2+</sup> loaded into mesoporous SiO<sub>2</sub> nanoparticles and luminol incorporated into luminol@MIL-53 on the upper and lower anodic poles of BPEs in separate chambers of a bipolar cell using a digital camera. An identical electric potential was applied across the bipolar electrodes, driving the reduction of thionine acetate at the cathodic poles and thereby facilitating simultaneous light emission of the luminophores. Moreover, the incorporation of electroactive Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> MXene-TiO<sub>2</sub> nanosheets catalyzed the electro-oxidation of co-reactants within both the Ru(bpy)<sub>3</sub><sup>2+</sup>/TPrA and luminol/H<sub>2</sub>O<sub>2</sub> systems, thereby enhancing the signal-to-background ratio and eliciting a significantly amplified visual ECL response. Under optimized conditions, the immunosensor displayed a linear response across a broad range of 100 pg mL<sup>−1</sup> to 400 ng/mL for Nanog, with a detection limit of 21.58 pg mL<sup>−1</sup>, and a linear response from 200 pg mL<sup>−1</sup> to 400 ng/mL with a detection limit of 106.24 pg mL<sup>−1</sup> for Oct-4. This study supports the immunosensor's potential for broader clinical application, where its high specificity and sensitivity could offer significant benefits in cancer diagnostics and stem cell research.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117482"},"PeriodicalIF":10.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143850247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situ dual-activated NIRF/PA carrier-free nanoprobe for diagnosis and treatment of Parkinson's disease

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-04-15 DOI: 10.1016/j.bios.2025.117473

Lixia Guo , Xiaowan Li , Run Zhang , Yixuan Hou , Bolong Ma , Zheng Li , Jiye Lv , Bin Wang , Sufang Ma , Lihong Li , Lili Yan , Boye Zhang , Wen Liu , Kahleong Lim , Haipeng Diao , Shaowei Wang , Chengwu Zhang

Parkinson's disease (PD) is the second most common neurodegenerative disease threatening the life of millions people worldwide. Oxidative stress, mitochondrial dysfunction, and neuroinflammation are the pivotal causative elements of PD. Precise diagnosis enables timely monitoring initiation and progression of PD, thereby facilitating the formulation of customized and targeted treatment strategies. Optical imaging offers one alternative way for PD diagnosis. However, available diagnostic probes suffer from the inability to bypass the blood brain barrier (BBB). To accurately diagnose and effectively combat PD, there is an urgent need to develop an integrated diagnostic and therapeutic nanoprobe that can bypass the BBB and target the factors underlying degeneration of dopaminergic (DA) neurons. In present study, one integrated carrier-free nanoprobe HVCur-NPs towards those factors was designed and constructed. By modifying probe side chain with polypeptide, RVG29, we obtained brain-targeting HV-PEG-RVG29. It not only enables BBB penetration, but also produces near-infrared fluorescence (NIRF) and photoacoustic (PA) signals in cascade response to H₂O₂ and viscosity. The release of loaded curcumin (CUR) prevents oxidative stress, neuroinflammation and restore mitochondrial function so as to rescue PD phenotypes. In cellular PD model, HVCur-NPs generated NIRF/PA signals in response to elevated ROS and viscosity, and ameliorated cell apoptosis by eliminating ROS and restoring mitochondria function. Moreover, in mice PD model, HVCur-NPs realized in situ NIRF/PA imaging brain, and rescued DA neuron loss and restored the behavioral deficit of PD mice, without detectable biotoxicity. This carrier-free nanoprobe opens venues for integrated diagnosis and treatment of neurodegenerative diseases.

{"title":"In situ dual-activated NIRF/PA carrier-free nanoprobe for diagnosis and treatment of Parkinson's disease","authors":"Lixia Guo , Xiaowan Li , Run Zhang , Yixuan Hou , Bolong Ma , Zheng Li , Jiye Lv , Bin Wang , Sufang Ma , Lihong Li , Lili Yan , Boye Zhang , Wen Liu , Kahleong Lim , Haipeng Diao , Shaowei Wang , Chengwu Zhang","doi":"10.1016/j.bios.2025.117473","DOIUrl":"10.1016/j.bios.2025.117473","url":null,"abstract":"<div><div>Parkinson's disease (PD) is the second most common neurodegenerative disease threatening the life of millions people worldwide. Oxidative stress, mitochondrial dysfunction, and neuroinflammation are the pivotal causative elements of PD. Precise diagnosis enables timely monitoring initiation and progression of PD, thereby facilitating the formulation of customized and targeted treatment strategies. Optical imaging offers one alternative way for PD diagnosis. However, available diagnostic probes suffer from the inability to bypass the blood brain barrier (BBB). To accurately diagnose and effectively combat PD, there is an urgent need to develop an integrated diagnostic and therapeutic nanoprobe that can bypass the BBB and target the factors underlying degeneration of dopaminergic (DA) neurons. In present study, one integrated carrier-free nanoprobe HVCur-NPs towards those factors was designed and constructed. By modifying probe side chain with polypeptide, RVG29, we obtained brain-targeting HV-PEG-RVG29. It not only enables BBB penetration, but also produces near-infrared fluorescence (NIRF) and photoacoustic (PA) signals in cascade response to H<sub>2</sub>O<sub>2</sub> and viscosity. The release of loaded curcumin (CUR) prevents oxidative stress, neuroinflammation and restore mitochondrial function so as to rescue PD phenotypes. In cellular PD model, HVCur-NPs generated NIRF/PA signals in response to elevated ROS and viscosity, and ameliorated cell apoptosis by eliminating ROS and restoring mitochondria function. Moreover, in mice PD model, HVCur-NPs realized in situ NIRF/PA imaging brain, and rescued DA neuron loss and restored the behavioral deficit of PD mice, without detectable biotoxicity. This carrier-free nanoprobe opens venues for integrated diagnosis and treatment of neurodegenerative diseases.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"282 ","pages":"Article 117473"},"PeriodicalIF":10.7,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D printed shamrock-like electrochemical biosensing tool based on enzymatic inhibition for on-line nerve agent measurement in drinking water

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-04-15 DOI: 10.1016/j.bios.2025.117471

Ludovica Gullo , Beatrice Brunelleschi , Leonardo Duranti , Luca Fiore , Vincenzo Mazzaracchio , Fabiana Arduini

Terrorist attacks using chemical warfare agents have been unfortunately well documented in history, and among chemical warfare agents, nerve agents are the preferred ones due to their ease of synthesis and transport. A huge issue is the contamination of drinking water by adding nerve agents into aqueducts, making their on-line monitoring an urgent need to ensure the safety of drinking water. A compact and portable shamrock-like electrochemical inhibitive biosensor was fabricated by 3D printing the entire electrochemical cell and the flow cell for on-line nerve agent measurements. Paraoxon was selected as the nerve agent simulant and detected by harnessing its capability to inhibit irreversibly the butyrylcholinesterase enzyme. This enzyme was chemically immobilized onto the 3D printed electrode previously modified by drop casting with Carbon Black-Prussian Blue nanoparticles to ensure the detection of enzymatic by-product thiocholine at low applied potential (+300 mV vs. Ag/AgCl), with enhanced selectivity and sensitivity. This 3D printed analytical tool demonstrated a detection limit as low as 0.9 ppb and 1.6 ppb with a linear range of up to 20 ppb in standard solution and untreated tap water, respectively. The accuracy was evaluated by the recovery study, obtaining recovery values comprised in the range of 99–105 %. These results demonstrated the effectiveness of the combination of 3D printing technology, nanomaterials, and electrochemical sensing to deliver a customized on-line device able to work as a smart warning system for environmental surveillance and public health protection.

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引用次数: 0

Balancing performance and stability characteristics in organic electrochemical transistor

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics

Pub Date : 2025-04-14 DOI: 10.1016/j.bios.2025.117476

Nikolay Mukhin , Andreas Dietzel , Vadim Issakov , Liubov Bakhchova

Nowadays organic electrochemical transistors (OECTs) are becoming a promising platform for bioelectronics and biosensing due to its biocompatibility, high sensitivity and selectivity, low driving voltages, high transconductance and flexibility. However, the existing problems associated with degradation processes within the OECT during long-term operation hinder their widespread implementation. Moreover, trade-offs often arise between OECT transconductance and speed, fast ion transport and electron mobility, electrochemical stability and sensitivity, cycling stability and signal amplification, and other metrics. Ensuring high performance characteristics and achieving enhanced stability in OECTs are distinct strategies that do not always align, as progress in one aspect often necessitates a trade-off with the other. This dynamic arises from the need to find a balance between reversible and irreversible processes in the behavior of OECT active layers, and providing simultaneously favorable conditions for ion and electron transport and their efficient charge coupling. This review article systematically summarizes the phenomenological and physical-chemical aspects associated with factors and mechanisms that determine both performance and long-term stability of OECT, paying special attention to the consideration of existing and promising approaches to extend the OECT lifespan, while maintaining (or even increasing) high effectiveness of its operation.

有机电化学晶体管（OECT）具有生物兼容性、高灵敏度和高选择性、低驱动电压、高跨导率和灵活性等特点，正逐渐成为生物电子学和生物传感领域前景广阔的平台。然而，OECT 在长期运行过程中出现的降解问题阻碍了其广泛应用。此外，在 OECT 的跨导和速度、快速离子传输和电子迁移率、电化学稳定性和灵敏度、循环稳定性和信号放大率以及其他指标之间，经常会出现权衡取舍的问题。在 OECT 中确保高性能特性和实现更高的稳定性是不同的策略，但并不总是一致的，因为一个方面的进步往往需要与另一个方面进行权衡。这种动态变化源于需要在 OECT 活性层行为的可逆和不可逆过程之间找到平衡，并同时为离子和电子传输及其高效电荷耦合提供有利条件。这篇综述文章系统地总结了与决定 OECT 性能和长期稳定性的因素和机制有关的现象学和物理化学方面的问题，特别关注考虑现有的和有前景的方法，以延长 OECT 的使用寿命，同时保持（甚至提高）其运行的高效性。

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