Luminescence “on-off-on” sensing of acute myocardial infarction biomarker miRNA-208a based on copper nanoclusters-MoS2 FRET system

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-02-15 DOI:10.1007/s00604-024-06940-x

Suzhen Zhang, Danling Mei, Hongming Fang, Quanwei Li, Xiumei Zhou, Xueqin Zhao, Zhencang Zheng, Biao Huang

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Abstract

MiRNA-208a is a molecular marker for the early stage of acute myocardial infarction (AMI), with satisfactory specificity and a relatively short time window, making it highly suitable for the early diagnosis of AMI. In this study, a CuNCs-cDNA-MoS₂ fluorescent probe is designed and a Förster resonance energy transfer (FRET) enzyme-free fluorescent biosensor is established for “on-off-on” detection of miRNA-208a. The results showed that the detection limit for miRNA-208a was 900 pM, with a linear range of 1 to 10 nM. Interestingly, after the addition of RNase inhibitor, the detection time and limit for serum miRNA-208a were reduced to 5 min and 380 pM, respectively. It also has a wide linear range (1-20 nM), with a recovery of 98.13 to 101.20%. Compared to traditional methods, this strategy is simple, rapid, efficient, and cost-effective. In summary, the FRET-based fluorescent sensor provides a high-performance method for detecting miRNA-208a.

Graphical Abstract

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基于纳米铜簇-MoS2 FRET 系统的急性心肌梗死生物标记物 miRNA-208a 的发光 "开关 "传感技术

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.