Nutrient removal performance and microbial composition analysis in hybrid membrane bioreactor for municipal wastewater treatment.

Abstract

The removal of nutrients from wastewater to reduce the toxicity of these compounds to the environment requires more space in wastewater treatment plants to establish anaerobic, anoxic and aerobic treatment stages. To address this limitation, researchers have developed practical, intensive hybrid treatment systems that enhance nutrient removal performance while requiring less space. However, the implementation of hybrid systems within a reactor introduces the interaction between the attached and suspended growth that can influence the microbial community structure and the performance of the system, so it is crucial to understand the composition of the microbial communities involved in hybrid growth to optimize control strategies in these systems. This study investigated the microbial community structure of the integrated moving bed membrane bioreactor (IMBMBR) system and its impact on nutrient removal in municipal wastewater. The findings demonstrated that the effluent quality was improved with the IMBMBR. The efficiency of removing COD, BOD₅, ${\text{NH}}_4^{+}\text{-N}$ and ${\text{PO}}_4^{3-}\text{-P}$ in the IMBMBR were 91 ± 4.0%, 95 ± 4.0%, 99 ± 0.2% and 24 ± 3.0%, respectively. The IMBMBR had better nitrite oxidation and complete nitrification by increasing the diversity and abundance of effective bacteria. The abundance of Proteobacteria, Bacteroidetes and Nitrospira was enhanced in IMBMBR. Coexistence of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in IMBMBR led to increased nutrient removal. The study results suggest that IMBMBR can be an effective process for nutrient removal, achieving quality standards that comply with legal requirements for wastewater in municipal and industries with limited space for establishing treatment facilities. Additionally, this process can be quickly implemented as an upgrade to existing wastewater treatment plants, avoiding the need to develop an entirely new system.