Bioimaging insights into structural pathways of cell-to-cell communication within the male (MGU) and female (FGU) germ units of Arabidopsis thaliana.

Abstract

Key message: Cytoplasmic connections are present between cells within male and female germ units (MGU, FGU), suggesting potential structural pathways for communication. Cell-to-cell communication within the male germ unit (MGU), which consists of two sperm cells and the vegetative cell nucleus, and the female germ unit (FGU), comprising the synergids, the egg cell, and the central cell, is crucial for gamete maturation, fertilization, and early embryogenesis in angiosperms. The MGU facilitates the transport and delivery of immotile sperm cells via the elongating pollen tube to the FGU/embryo sac, which is deeply embedded within the ovule and the ovary. Through applying various bioimaging techniques at both electron and light microscopy levels, we examine the structure and the function of these units in the model plant Arabidopsis thaliana, with a particular focus on potential structural pathways for communication. In the MGU, this communication is facilitated by a cytoplasmic projection that connects the sperm cells to the lobed vegetative nucleus. In the FGU, the extracellular matrix adjacent to the egg cell, central cell, and synergids plays a similar role. We discuss our findings in the context of previous studies on Hyacinthus orientalis, where, in contrast to Arabidopsis-which possesses a tricellular pollen structure-sperm cells are formed within the growing pollen tube.