Portable vibrational spectroscopy instruments and chemometrics for the classification of cotton fibers according the length (UHM)

Abstract

In this study, novel methods using portable NIR and Raman spectroscopy instruments associated with multivariate classification were developed to classify cotton fibers according to their length. The Upper Half Mean (UHM) length is considered a quality parameter by the cotton fiber market and is traditionally determined using a high-volume system (HVI), which entails high installation costs and labor-intensive analyses. As UHM correlates with cellulose polymerization, its determination can be achieved through vibrational spectroscopy techniques such as near-infrared (NIR) and Raman. These technologies offer advantages such as low cost, ease of handling, and rapid data acquisition, making them suitable for field use. This study aimed to develop a method and demonstrate the feasibility of using portable NIR and Raman spectrometers coupled with pattern recognition (PR) methods for routine analysis of cotton fibers, serving as a proof of concept for practical application in the industry. A total of 142 samples of cotton fibers from cotton improvement experiments conducted by the Brazilian Agricultural Research Corporation (EMBRAPA) were employed. Two classification approaches based on cotton lint length and the related economic value were employed. The first aimed to differentiate between short (SM) and long (LF) fibers, while the second aimed to further classify long fibers into internal classes (L, VL, and EL). Overall, methods using portable Raman spectrometer exhibited 100% accuracy performance regardless of the PR technique used. Meanwhile, methods based on NIR spectrometers achieved accuracies of 100% depending on the PR method and variable selection employed. The use of GLSW resulted in a reduction of a latent variable. In conclusion, the use of portable NIR and Raman spectrometers combined with PR methods emerges as an innovative and viable technology for the classification of cotton fibers based on their length.