Nitrate activates an MKK3-dependent MAPK module via NLP transcription factors in Arabidopsis

IF 6.2 1区 生物学 Q1 PLANT SCIENCES The Plant Journal Pub Date : 2025-02-17 DOI:10.1111/tpj.70010
Sebastian T. Schenk, Virginie Brehaut, Camille Chardin, Marie Boudsocq, Anne Marmagne, Jean Colcombet, Anne Krapp
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Abstract

Plant responses to nutrient availability are critical for plant development and yield. Nitrate, the major form of nitrogen in most soils, serves as both a nutrient and signaling molecule. Nitrate itself triggers rapid, major changes in gene expression, especially via nodule inception (NIN)-like protein (NLP) transcription factors, and stimulates protein phosphorylation. Mitogen-activated protein kinase (MAPK)-related genes are among the early nitrate-responsive genes; however, little is known about their roles in nitrate signaling pathways. Here, we show that nitrate resupply to nitrogen-depleted Arabidopsis (Arabidopsis thaliana) plants triggers, within minutes, an MAPK cascade that requires NLP-dependent transcriptional induction of mitogen-activated protein kinase kinase kinase 13 (MAP3K13) and MAP3K14 and that the MAPK cascade is composed of MKK3 and likely C-clade MAPKs (MPK1/2/7/14). Importantly, nitrate reductase-deficient mutants exhibited nitrate-induced MPK7 activities comparable to those observed in wild-type plants, indicating that nitrate itself is the signal that stimulates the cascade. We show that the modified expression of MAP3K13 and MAP3K14 affects nitrate-stimulated BT2 expression and modulates plant responses to nitrogen availability, such as nitrate uptake and senescence. Our finding that an MAPK cascade involving MAP3K13 and MAP3K14 functions in the complex regulatory network governing responses to nitrate availability will guide future strategies to optimize plant responses to nitrogen fertilization and nitrogen use efficiency.

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植物对养分供应的反应对植物的生长发育和产量至关重要。硝酸盐是大多数土壤中氮的主要形式,既是一种营养物质,也是一种信号分子。硝酸盐本身会引发基因表达的快速、重大变化,特别是通过类似节点萌发蛋白(NIN)的转录因子,并刺激蛋白质磷酸化。与丝裂原活化蛋白激酶(MAPK)相关的基因是早期硝酸盐反应基因之一;然而,人们对它们在硝酸盐信号通路中的作用知之甚少。在这里,我们发现硝酸盐补给缺氮的拟南芥(Arabidopsis thaliana)植株会在几分钟内触发一个 MAPK 级联,该级联需要 NLP 依赖性转录诱导丝裂原活化蛋白激酶激酶 13(MAP3K13)和 MAP3K14,并且该 MAPK 级联由 MKK3 和可能的 C 支系 MAPK(MPK1/2/7/14)组成。重要的是,硝酸盐还原酶缺陷突变体表现出的硝酸盐诱导的 MPK7 活性与在野生型植物中观察到的活性相当,这表明硝酸盐本身就是刺激级联的信号。我们的研究表明,MAP3K13 和 MAP3K14 的表达改变会影响硝酸盐刺激的 BT2 表达,并调节植物对氮供应的反应,如硝酸盐吸收和衰老。我们的发现表明,MAP3K13 和 MAP3K14 参与的 MAPK 级联在管理对硝酸盐可用性的反应的复杂调控网络中起作用,这将指导未来优化植物对氮肥和氮利用效率的反应的策略。
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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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