High Internal Phase Emulsion-Templated Hydrophilic Polyphosphoester Scaffolds: Tailoring the Porosity and Degradation for Soft Tissue Engineering.

IF 5.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Biomacromolecules Pub Date : 2025-02-16 DOI:10.1021/acs.biomac.4c01740

Pascal Boucq, Bernard Ucakar, Floriane Debuisson, Raphael Riva, Anne des Rieux, Christine Jérôme, Antoine Debuigne

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引用次数: 0

Abstract

Synthetic porous scaffolds are key elements in tissue engineering (TE), requiring controlled porosity for cell colonization, along with a degradation rate aligned with tissue growth. While biodegradable polyester scaffolds are widely used in TE, they are primarily hydrophobic and suited for semirigid to hard tissue applications. This work broadens the scope of TE by introducing porous scaffolds made of polyphosphoesters (PPEs), degradable polymers with adaptable physicochemical properties. PPE hydrogels were shaped into 3D scaffolds using an emulsion templating method, yielding hydrophilic matrices with controlled porosity and tunable Young's moduli for soft tissues. Degradation assays at physiological pH confirmed the scaffolds' biodegradability. Cytotoxicity tests with PPE scaffolds showed excellent cell viability, while RGD functionalization further enhanced cell adhesion. Scaffold colonization, low inflammation, and angiogenesis were demonstrated in vivo through subcutaneous implantation of the scaffolds in mice and histological analysis. These results highlight PPE-based scaffolds as promising candidates for regenerative medicine.

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来源期刊

Biomacromolecules 化学-高分子科学

CiteScore

10.60

自引率

4.80%

发文量

417

审稿时长

1.6 months

期刊介绍： Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.