High Internal Phase Emulsion-Templated Hydrophilic Polyphosphoester Scaffolds: Tailoring the Porosity and Degradation for Soft Tissue Engineering

IF 5.4 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Biomacromolecules Pub Date : 2025-03-10 Epub Date: 2025-02-17 DOI:10.1021/acs.biomac.4c01740

Pascal Boucq , Bernard Ucakar , Floriane Debuisson , Raphael Riva , Anne des Rieux , Christine Jérôme , Antoine Debuigne

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Abstract

Synthetic porous scaffolds are key elements in tissue engineering (TE), requiring controlled porosity for cell colonization, along with a degradation rate aligned with tissue growth. While biodegradable polyester scaffolds are widely used in TE, they are primarily hydrophobic and suited for semirigid to hard tissue applications. This work broadens the scope of TE by introducing porous scaffolds made of polyphosphoesters (PPEs), degradable polymers with adaptable physicochemical properties. PPE hydrogels were shaped into 3D scaffolds using an emulsion templating method, yielding hydrophilic matrices with controlled porosity and tunable Young’s moduli for soft tissues. Degradation assays at physiological pH confirmed the scaffolds’ biodegradability. Cytotoxicity tests with PPE scaffolds showed excellent cell viability, while RGD functionalization further enhanced cell adhesion. Scaffold colonization, low inflammation, and angiogenesis were demonstrated in vivo through subcutaneous implantation of the scaffolds in mice and histological analysis. These results highlight PPE-based scaffolds as promising candidates for regenerative medicine.

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高内相乳液模板亲水性聚磷酸酯支架：为软组织工程量身定制孔隙度和降解。

合成多孔支架是组织工程（TE）的关键元素，需要控制孔隙度以进行细胞定植，以及与组织生长一致的降解率。虽然生物可降解聚酯支架广泛用于TE，但它们主要是疏水性的，适合半刚性到硬组织的应用。这项工作通过引入由聚磷酸酯（ppe）制成的多孔支架，拓宽了TE的范围，聚磷酸酯是具有适应性物理化学性质的可降解聚合物。使用乳液模板法将PPE水凝胶成型为3D支架，得到孔隙度可控、杨氏模量可调的软组织亲水性基质。生理pH下的降解实验证实了支架的生物降解性。细胞毒性实验显示PPE支架具有良好的细胞活力，而RGD功能化进一步增强了细胞粘附。通过小鼠皮下植入支架和组织学分析，证明支架定植、低炎症和血管生成。这些结果突出了基于聚乙烯的支架作为再生医学的有前途的候选者。

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来源期刊

Biomacromolecules 化学-高分子科学

CiteScore

10.60

自引率

4.80%

发文量

417

审稿时长

1.6 months

期刊介绍： Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.