Critical role for heat shock protein 70 in viral replication of ALV-J via interaction with gp37 and P32

IF 2.7 2区农林科学 Q3 MICROBIOLOGY Veterinary microbiology Pub Date : 2025-04-01 Epub Date: 2025-02-16 DOI:10.1016/j.vetmic.2025.110436

Kensi Zhu , Yanling Pang , Zhihong Luo , Hanyue Zhang, Shuang Tang, Jinjie Liang, Yuecheng Long, Wencheng Lin

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Abstract

Avian leukemia virus subgroup J (ALV-J) causes various diseases associated with tumor formation, decreased fertility and immunosuppression, resulting in significant economic losses in the poultry industry globally. Virus usually exploits the host cellular machinery for their replication. Although there are increasing evidences for the cellular proteins involving viral replication, the interaction between ALV-J and host proteins leading to the pivotal steps of viral life cycle are still unclear. Here, we reported that the heat shock protein 70 (Hsp70) plays a critical role during ALV-J infection by interacting with gp37 and P32. Changing the expression of Hsp70 affects the replication of ALV-J in host cells, and inhibitory of Hsp70 using the specific inhibitors JG-98 or Pifithrin-μ significantly reduced viral replication. This study revealed the effect of host Hsp70 on ALV-J replication, providing insights for further studies of the molecular mechanism of ALV-J infection.

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热休克蛋白70通过与gp37和P32相互作用在ALV-J病毒复制中的关键作用

禽白血病病毒J亚群（ALV-J）引起与肿瘤形成、生育力下降和免疫抑制相关的各种疾病，给全球家禽业造成重大经济损失。病毒通常利用宿主的细胞机制进行复制。尽管越来越多的证据表明细胞蛋白参与病毒复制，但ALV-J与宿主蛋白之间的相互作用导致病毒生命周期的关键步骤仍不清楚。在这里，我们报道了热休克蛋白70 （Hsp70）通过与gp37和P32相互作用在ALV-J感染中起关键作用。改变Hsp70的表达可影响宿主细胞中ALV-J的复制，特异性抑制剂JG-98或聚氟乙烯酯-μ抑制Hsp70可显著降低病毒复制。本研究揭示了宿主Hsp70对ALV-J复制的影响，为进一步研究ALV-J感染的分子机制提供了思路。

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.