Neuroprotective studies of Melatonin functionalized magnesium oxide nanoparticles

Abstract

Melatonin and magnesium nanoparticles exhibit diverse pharmacological properties. Trimethyltin and trimethyltin derived compounds both are toxic to neurons. The proposed research work is focus on synthesis on novel magnesium oxide nanoparticles conjugated with melatonin and evaluate its neuroprotective efficacy against trimethyltin induced neurotoxicity. Sol-gel method was used to fabricate Melatonin-MgO nanoparticles. Synthesized Melatonin-MgO particle were spectrally characterized. UV spectral analysis showed lambda max of Melatonin-MgO at 342 nm. Particle size analysis indicated that M − MgO nanoparticles have Z-average of 160.6 nm. Zeta potential estimation revealed that M − MgO exhibited Zeta potential of −0.1 mV. XRD analysis of M − MgO nanoparticles indicated that it has amorphous nature. SEM images revealed the agglomerated state of the particles. The cytotoxicity studies revealed that the IC₅₀ value of M − MgO against L929 mouse fibroblast cell line for 24 h treatment was found to be 77.02 μg/ml. The results of this investigation indicate that the Neuroprotective studies revealed that melatonin conjugated magnesium oxide was exhibited maximum neuroprotection rendering cell viability of 88.59 % at 30 μg/ml against trimethyltin induced neurointoxication in SK-N-SH cells. By utilizing the distinct neuroprotective properties of both substances, melatonin-functionalized magnesium oxide nanoparticles may provide a more efficient and focused method of treating oxidative stress and neuronal dysfunction in a range of neurological conditions.