Dayong Li , Wenting Cheng , Feifan Yin , Yanheng Yao , Zhongyun Wang , Yang Xiang
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引用次数: 0
Abstract
This study presents a novel method for sensitive miRNA detection based on a split-T7 switch modulating CRISPR/Cas12a system. By integrating the split-T7 promoter-mediated transcription with the CRISPR/Cas12a system, this method can achieve femtomolar detection of the target miRNA within 1 h and successfully analyze miR-21 in samples from various cell lines, demonstrating its potential for clinical applications.
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