{"title":"A sensitive miRNA detection method based on split-T7 switch modulating CRISPR/Cas12a system","authors":"Dayong Li, Wenting Cheng, Feifan Yin, Yanheng Yao, Zhongyun Wang, Yang Xiang","doi":"10.1039/d5cc00170f","DOIUrl":null,"url":null,"abstract":"This study presents a novel method for sensitive miRNA detection based on split-T7 switch modulating CRISPR/Cas12a system. By integrating the split-T7 promoter-mediated transcription with CRISPR/Cas12a system, this method can achieve femtomolar detection of the target miRNA within 1 h and successfully analyze miR-21 in samples from various cell lines, demonstrating its potential for clinical applications.","PeriodicalId":67,"journal":{"name":"Chemical Communications","volume":"13 1","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Communications","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d5cc00170f","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
This study presents a novel method for sensitive miRNA detection based on split-T7 switch modulating CRISPR/Cas12a system. By integrating the split-T7 promoter-mediated transcription with CRISPR/Cas12a system, this method can achieve femtomolar detection of the target miRNA within 1 h and successfully analyze miR-21 in samples from various cell lines, demonstrating its potential for clinical applications.
期刊介绍：
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