Optimization of culturing conditions for leafcutter ant associated, antimicrobial producing Pseudonocardia by adding ants´ cuticular hydrocarbons

IF 1.6 3区环境科学与生态学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Chemoecology Pub Date : 2024-11-22 DOI:10.1007/s00049-024-00414-5

Johannes-Paul Fladerer-Grollitsch, Juliana Fitzek, Kristina M. Sefc, Franz Bucar

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Abstract

The purpose of the present study was to investigate the influence of cultivation parameters on the growth of leafcutter ant associated actinomycete Pseudonocardia sp. These bacteria live in a very specialized symbiosis with the leafcutter ant Acromyrmex octospinosus and fungi of the genus Leucoagaricus. Pseudonocardia are known to produce antibiotics but are very hard to grow as a result of the complexity of their symbiosis. The bacteria were grown on yeast-malt-extract agar and the influence of pH, temperature conditions and the addition of chitin and a cuticular hydrocarbon (CHC) solution specific to leafcutter ants on the growth of Pseudonocardia and other bacteria inhabiting the leafcutter ants´ surface was tested. The best growth rate of Pseudonocardia was achieved by cultivation at 28 °C in a medium with pH 7 supplemented with chitin and CHC solution. Additionally, these conditions inhibited the growth of other competitive bacteria. This is the first report on the optimization of growth media for leafcutter ant associated Pseudonocardia adjusted to the conditions found on the ants´ cuticle.

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来源期刊

Chemoecology 环境科学-生化与分子生物学

CiteScore

4.20

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： It is the aim of Chemoecology to promote and stimulate basic science in the field of chemical ecology by publishing research papers that integrate evolution and/or ecology and chemistry in an attempt to increase our understanding of the biological significance of natural products. Its scopes cover the evolutionary biology, mechanisms and chemistry of biotic interactions and the evolution and synthesis of the underlying natural products. Manuscripts on the evolution and ecology of trophic relationships, intra- and interspecific communication, competition, and other kinds of chemical communication in all types of organismic interactions will be considered suitable for publication. Ecological studies of trophic interactions will be considered also if they are based on the information of the transmission of natural products (e.g. fatty acids) through the food-chain. Chemoecology further publishes papers that relate to the evolution and ecology of interactions mediated by non-volatile compounds (e.g. adhesive secretions). Mechanistic approaches may include the identification, biosynthesis and metabolism of substances that carry information and the elucidation of receptor- and transduction systems using physiological, biochemical and molecular techniques. Papers describing the structure and functional morphology of organs involved in chemical communication will also be considered.