OsWNK9 mitigates salt stress by promoting root growth and stomatal closure in rice.

Abstract

Salinity stress severely affects rice growth and reduces its productivity. With No Lysine Kinases (WNKs) are serine/threonine kinases emerging as potential candidate genes due to their involvement in various abiotic stress tolerance responses. However, studies providing mechanistic insights into the roles of WNKs in plants remain scarce. In the present study, OsWNK9-overexpressing rice lines showed strong tolerance to salinity stress. Overexpression of OsWNK9 also triggered the accumulation of abscisic acid (ABA) and restored indole-3-acetic acid (IAA) concentrations in roots, triggering stomatal closure in shoots and maintaining cell expansion of the root epidermal cells when challenged with salt treatment. The overexpression lines showed increased activity of antioxidant enzymes, which further mitigated ROS-mediated cellular damage under salinity stress. We also identified that OsWNK9 interacts with Receptor for Activated Kinase C1A (RACK1A), ABA-8'-hydroxylase, and (Vacuolar Type ATPase) V-Type ATPase. Taken together, our findings suggest that OsWNK9 expression is warranted under salinity stress and exerts its effects by interacting with its downstream targets and by increased accumulation of ABA and IAA, thereby regulating seed germination, stomatal activity, improved root growth, and ionic homeostasis, which all contribute to significantly higher yield produced per plant under long term salinity stress.