Lili Quan, Qianrong Yang, Zhu Jin, Rong Li, Han Xiao, Lu Wang
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引用次数: 0
Abstract
Background: Tuberculosis (TB) is a global health challenge from a single infectious agent, Mycobacterium tuberculosis (MTB), and it demands improved diagnostics and therapies.
Objective: This work explored a novel method for detecting MTB by combining nanogold labeling (NGL) technology with silver staining to enhance sensitivity and specificity.
Methods: Nanogold particles (NGPs) were characterized using ultraviolet absorption spectroscopy (UVAS), and their morphology was observed via transmission electron microscopy (TEM). The silver staining enhancement (SSE) system was optimized for a reaction time of 11 min. Fifty drug-resistant tuberculosis (DRT) patients were randomly assigned to a control (Ctrl) group receiving conventional nursing and an experimental (Exp) group treated with continuous nursing intervention (CNI). Quality of Life Instrument for Tuberculosis Patients (QLI-TB) scores were compared over 6 months.
Results: Unmarked NGPs were evenly distributed, while labeled NGPs maintained complete morphology with a gray halo. The detection limit was established at 0.582, reaching as low as 1 pmol/L. For sputum specimens, detection rates were 38.7% for culture, 41.94% for PCR, and 43.54% for nanogold SSE, with no significant differences (P > 0.05). However, patients in the Exp group exhibited significant improvements in physical, psychological, and social functions, as well as the tuberculosis-specific module (TSM) compared to the Ctrl group (P < 0.05).
Conclusions: We demonstrated an innovative method for detecting MTB, demonstrating promising results through method optimization and analysis.
期刊介绍：
The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.
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