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Extract from Falcaria vulgaris loaded with exosomes for the treatment of hypertension in pregnant mice: In vitro and In vivo investigations. 含有外泌体的法桐提取物用于治疗怀孕小鼠的高血压:体外和体内研究。
IF 1 4区 医学 Q4 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-30 DOI: 10.3233/BME-240053
Jing Chen, Huan Wang, Jing Zhu

Background: Hypertensive disorders during pregnancy pose significant risks to both maternal and fetal health, necessitating safe and effective therapeutic interventions.

Objective: This study aimed to investigate the potential of an extract derived from Falcaria vulgaris (FV), loaded with exosomes to form the Exo/FV complex, as a novel therapeutic agent for the management of hypertension in pregnant mice: antioxidants, antimicrobials, and phenolic compounds present in FV lower blood pressure.

Methods: The isolation of exosomes was done by ultracentrifugation methods and the FV was loaded into the exosomes by electroporation method.

Results: The Exo/FV was found to be spherical with diameter ranges from 20 to 30 nm and they were tested for biocompatibility in NHI 3T3 cell lines and found to be effective. This research investigated in vivo hypertension in mice induced by L-NAME and treated with FV and Exo/FV and found that AChE and MAO determine mice's redox state tends to reduce blood pressure. Increased non-protein thiol (NP-SH) and decreased lipid peroxidation were also found, and PDE-5, ACE, Arginase, and MDA activity has also been tested.

Conclusion: This analysis showed that Exo/FV effectively treated hypertension during pregnancy.

背景:妊娠期高血压疾病对母体和胎儿的健康构成重大风险,因此需要安全有效的治疗干预措施:本研究的目的是研究一种从蚕豆(Falcaria vulgaris,FV)中提取的提取物与外泌体形成 Exo/FV 复合物,作为一种新型治疗剂治疗妊娠小鼠高血压的潜力:FV 中的抗氧化剂、抗菌剂和酚类化合物可降低血压:方法:用超速离心法分离外泌体,用电穿孔法将 FV 加入外泌体:结果:外泌体/FV呈球形,直径在20至30纳米之间。这项研究调查了由 L-NAME 诱导并用 FV 和 Exo/FV 治疗的小鼠体内高血压情况,发现 AChE 和 MAO 决定小鼠的氧化还原状态,并倾向于降低血压。此外,还发现了非蛋白质硫醇(NP-SH)的增加和脂质过氧化的减少,并检测了 PDE-5、ACE、精氨酸酶和 MDA 的活性:分析表明,Exo/FV 能有效治疗妊娠期高血压。
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引用次数: 0
Acupuncture navigation method integrated with augmented reality. 与增强现实技术相结合的针灸导航方法。
IF 1 4区 医学 Q4 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-27 DOI: 10.3233/BME-240073
Shin-Yan Chiou, Meng-Ru He

Background: Acupuncture and moxibustion are effective in alleviating symptoms, but the large number of acupoints can make accurate needle placement and training difficult.

Objective: To address these challenges, this study aims to develop an augmented reality (AR) acupuncture navigation system designed to improve the accuracy and intuitiveness of acupoint localization.

Methods: The proposed system employs a six-point registration and positioning technique, enabling the AR navigation model to adapt to the specific characteristics of each patient.

Results: In testing, discrepancies between virtual and actual acupuncture points ranged from 0.6 mm to 3.9 mm, which is within the acceptable tolerance range for acupuncture.

Conclusion: This AR-based system shows promise in enhancing the precision of acupuncture point localization, potentially leading to improved treatment outcomes.

背景:针灸能有效缓解症状,但由于穴位众多,准确下针和训练十分困难:为了应对这些挑战,本研究旨在开发一种增强现实(AR)针灸导航系统,以提高穴位定位的准确性和直观性:方法:拟议的系统采用了六点注册和定位技术,使 AR 导航模型能够适应每位患者的具体特征:在测试中,虚拟穴位与实际穴位之间的差异从 0.6 毫米到 3.9 毫米不等,在针灸可接受的误差范围内:结论:这一基于 AR 的系统有望提高穴位定位的精确度,从而改善治疗效果。
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引用次数: 0
Promotion of maturation in CDM3-induced embryonic stem cell-derived cardiomyocytes by palmitic acid. 棕榈酸促进 CDM3 诱导的胚胎干细胞衍生心肌细胞的成熟。
IF 1 4区 医学 Q4 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-26 DOI: 10.3233/BME-240101
Junsheng Mu, Zhen Gao, Ping Bo, Bin You

Background: Myocardial infarction leads to myocardial necrosis, and cardiomyocytes are non-renewable. Fatty acid-containing cardiomyocyte maturation medium promotes maturation of stem cell-derived cardiomyocytes.

Objective: To study the effect palmitic acid on maturation of cardiomyocytes derived from human embryonic stem cells (hESCs) to optimize differentiation for potential treatment of myocardial infarction by hESCs.

Methods: hESCs were differentiated into cardiomyocytes using standard chemically defined medium 3 (CDM3). Up to day 20 of differentiation, 200 Mm palmitic acid were added, and then the culture was continued for another 8 days to mimic the environment in which human cardiomyocytes mainly use fatty acids as the main energy source. Light microscopy, transmission electron microscopy, immunofluorescence, reverse transcription-polymerase chain reaction, and cellular ATP assays, were carried out to analyze the expression of relevant cardiomyocyte-related genes, cell morphology, metabolism levels, and other indicators cardiomyocyte maturity.

Results: Cardiomyocytes derived from hESCs under exogenous palmitic acid had an elongated pike shape and a more regular arrangement. Sarcomere stripes were clear, and the cells color was clearly visible. The cell perimeter and elongation rate were also increased. Myogenic fibers were abundant, myofibrillar z-lines were regularly, the numbers of mitochondria and mitochondrial cristae were higher, more myofilaments were observed, and the structure of round-like discs was occasionally seen. Expression of mature cardiomyocyte-associated genes TNNT2, MYL2 and MYH6, and cardiomyocyte-associated genes KCNJ4, RYR2,and PPARα, was upregulated (p < 0.05). Expression of MYH7, MYL7, KCND2, KCND3, GJA1 and TNNI1 genes was unaffected (p > 0.05). Expression of mature cardiomyocyte-associated sarcomere protein MYL2 was significantly increased (p < 0.05), MYH7 protein expression was unaffected (p > 0.05). hESC-derived cardiomyocytes exposed to exogenous palmitic acid produced more ATP per unit time (p < 0.05).

Conclusion: Exogenous palmitic acid induced more mature hESC-CMs in terms of the cellular architecture, expression of cardiomyocyte maturation genes adnprotein, and metabolism.

背景:心肌梗死导致心肌坏死,而心肌细胞是不可再生的。含脂肪酸的心肌细胞成熟培养基可促进干细胞衍生心肌细胞的成熟:研究棕榈酸对人类胚胎干细胞(hESCs)衍生的心肌细胞成熟的影响,以优化分化,从而利用 hESCs 治疗心肌梗死。方法:使用标准化学定义培养基 3(CDM3)将 hESCs 分化成心肌细胞。方法:使用标准化学定义培养基 3(CDM3)将 hESCs 分化成心肌细胞,在分化的第 20 天,加入 200 Mm 棕榈酸,然后继续培养 8 天,以模拟人类心肌细胞主要以脂肪酸为主要能量来源的环境。通过光学显微镜、透射电子显微镜、免疫荧光、反转录聚合酶链反应和细胞ATP测定等方法,分析心肌细胞相关基因的表达、细胞形态、代谢水平等心肌细胞成熟度指标:结果:在外源性棕榈酸的作用下,由 hESCs 培育出的心肌细胞呈拉长的梭形,排列更加规则。肌节条纹清晰,细胞颜色明显。细胞周长和伸长率也有所增加。肌原纤维丰富,肌纤维 Z 线规则,线粒体和线粒体嵴数量增多,肌丝增多,偶见圆盘状结构。成熟心肌细胞相关基因 TNNT2、MYL2 和 MYH6 以及心肌细胞相关基因 KCNJ4、RYR2 和 PPARα 表达上调(p 0.05)。暴露于外源棕榈酸的 hESC 衍生心肌细胞在单位时间内产生更多的 ATP(p 结论:外源棕榈酸诱导心肌细胞产生更多的 ATP(p 结论:外源棕榈酸诱导心肌细胞产生更多的 ATP(p 结论:外源棕榈酸诱导心肌细胞产生更多的 ATP(p 结论:外源棕榈酸诱导心肌细胞产生更多的 ATP(p 结论):外源性棕榈酸可诱导 hESC-CMs 在细胞结构、心肌细胞成熟基因 adnprotein 的表达和新陈代谢方面更加成熟。
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引用次数: 0
Assessment of decellularization strategy and biocompatibility testing of full-thickness abdominal wall to produce a tissue-engineered graft. 评估全厚腹壁的脱细胞策略和生物相容性测试,以制作组织工程移植物。
IF 1 4区 医学 Q4 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-26 DOI: 10.3233/BME-240144
George Skepastianos, Panagiotis Mallis, Epameinondas Kostopoulos, Efstathios Michalopoulos, Vasileios Skepastianos, Christos Doudakmanis, Chrysoula Palazi, Gerasimos Tsourouflis

Background: Restoration of the abdominal wall defects due to herniation or other complications represents a challenging task of the reconstructive surgery. Synthetic grafts or crosslinked animal-derived grafts, are utilized, followed by significant adverse reactions.

Objective: This study aimed to evaluate primarily the production of a decellularized abdominal wall scaffold and secondly its biocompatibility upon transplantation in an animal model.

Methods: Full-thickness abdominal wall samples were harvested from Wistar Rats and then decellularized utilizing a three-cycle process. To evaluate the decellularization efficacy, histological, biochemical and biomechanical analyses were performed. The biocompatibility assessment involved the implantation of the produced scaffolds to Sprague Dawley rats. The grafts remained for a total period of 4 weeks, followed by immunohistochemistry for the detection of CD11b+, CD4+ and CD8+ cells.

Results: Histological, biochemical and biomechanical results, indicated the production of compatible acellular full-thickness abdominal wall samples. After 4 weeks of implantation, a minor presence of immunity cells was observed.

Conclusion: The data of this study indicated the successful production of a full-thickness abdominal wall scaffold. Biologically derived full-thickness abdominal wall scaffolds may have greater potential in restoration of the abdominal wall defects, bringing them one step closer to their clinical utility.

背景:修复因疝气或其他并发症造成的腹壁缺损是整形外科的一项具有挑战性的任务。使用合成移植物或交联动物源移植物会产生严重的不良反应:本研究旨在首先评估脱细胞腹壁支架的生产情况,其次评估其在动物模型中移植时的生物相容性:方法:从 Wistar 大鼠身上采集全厚腹壁样本,然后采用三周期工艺进行脱细胞处理。为评估脱细胞效果,进行了组织学、生物化学和生物力学分析。生物相容性评估包括将制成的支架植入 Sprague Dawley 大鼠体内。移植物共放置了 4 周,然后用免疫组织化学方法检测 CD11b+、CD4+ 和 CD8+ 细胞:结果:组织学、生物化学和生物力学结果表明,制作出了兼容的无细胞全厚腹壁样本。植入 4 周后,观察到少量免疫细胞的存在:结论:这项研究的数据表明,全厚腹壁支架的制作是成功的。生物衍生全厚腹壁支架在修复腹壁缺损方面可能具有更大的潜力,使其离临床应用更近了一步。
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引用次数: 0
Effect of bioceramic inclusions on gel-cast aliphatic polymer membranes for bone tissue engineering applications: An in vitro study. 生物陶瓷夹杂物对用于骨组织工程的凝胶铸造脂肪族聚合物膜的影响:体外研究。
IF 1 4区 医学 Q4 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-26 DOI: 10.3233/BME-240079
Vasudev Vivekanand Nayak, Edmara T P Bergamo, Vijayavenkataraman Sanjairaj, Rakesh Kumar Behera, Nikhil Gupta, Paulo G Coelho, Lukasz Witek

Background: Polylactic acid (PLA) has been extensively used in tissue engineering. However, poor mechanical properties and low cell affinity have limited its pertinence in load bearing bone tissue regeneration (BTR) devices.

Objective: Augmenting PLA with β-Tricalcium Phosphate (β-TCP), a calcium phosphate-based ceramic, could potentially improve its mechanical properties and enhance its osteogenic potential.

Methods: Gels of PLA and β-TCP were prepared of different % w/w ratios through polymer dissolution in acetone, after which polymer-ceramic membranes were synthesized using the gel casting workflow and subjected to characterization.

Results: Gel-cast polymer-ceramic constructs were associated with significantly higher osteogenic capacity and calcium deposition in differentiated osteoblasts compared to pure polymer counterparts. Immunocytochemistry revealed cell spreading over the gel-cast membrane surfaces, characterized by trapezoidal morphology, distinct rounded nuclei, and well-aligned actin filaments. However, groups with higher ceramic loading expressed significantly higher levels of osteogenic markers relative to pure PLA membranes. Rule of mixtures and finite element models indicated an increase in theoretical mechanical strength with an increase in β-TCP concentration.

Conclusion: This study potentiates the use of PLA/β-TCP composites in load bearing BTR applications and the ability to be used as customized patient-specific shape memory membranes in guided bone regeneration.

背景:聚乳酸(PLA)已被广泛应用于组织工程。然而,较差的机械性能和较低的细胞亲和性限制了其在承重骨组织再生(BTR)装置中的相关性:目的:用β-磷酸三钙(β-TCP)(一种磷酸钙基陶瓷)增强聚乳酸有可能改善其机械性能并增强其成骨作用:方法:通过丙酮溶解聚合物,制备出不同重量比的聚乳酸和β-TCP凝胶,然后采用凝胶浇注工作流程合成聚合物陶瓷膜并进行表征:结果:与纯聚合物相比,凝胶浇铸聚合物陶瓷构建体的成骨能力和分化成骨细胞中的钙沉积明显更强。免疫细胞化学显示,细胞在凝胶浇铸膜表面铺展,具有梯形形态、明显的圆形细胞核和排列整齐的肌动蛋白丝。然而,与纯聚乳酸膜相比,陶瓷负载较高的组表达的成骨标记物水平明显更高。混合物和有限元模型的规则表明,随着β-TCP浓度的增加,理论机械强度也会增加:这项研究提高了聚乳酸/β-TCP 复合材料在承重 BTR 应用中的使用潜力,以及在引导骨再生中用作定制的患者特异性形状记忆膜的能力。
{"title":"Effect of bioceramic inclusions on gel-cast aliphatic polymer membranes for bone tissue engineering applications: An in vitro study.","authors":"Vasudev Vivekanand Nayak, Edmara T P Bergamo, Vijayavenkataraman Sanjairaj, Rakesh Kumar Behera, Nikhil Gupta, Paulo G Coelho, Lukasz Witek","doi":"10.3233/BME-240079","DOIUrl":"https://doi.org/10.3233/BME-240079","url":null,"abstract":"<p><strong>Background: </strong>Polylactic acid (PLA) has been extensively used in tissue engineering. However, poor mechanical properties and low cell affinity have limited its pertinence in load bearing bone tissue regeneration (BTR) devices.</p><p><strong>Objective: </strong>Augmenting PLA with β-Tricalcium Phosphate (β-TCP), a calcium phosphate-based ceramic, could potentially improve its mechanical properties and enhance its osteogenic potential.</p><p><strong>Methods: </strong>Gels of PLA and β-TCP were prepared of different % w/w ratios through polymer dissolution in acetone, after which polymer-ceramic membranes were synthesized using the gel casting workflow and subjected to characterization.</p><p><strong>Results: </strong>Gel-cast polymer-ceramic constructs were associated with significantly higher osteogenic capacity and calcium deposition in differentiated osteoblasts compared to pure polymer counterparts. Immunocytochemistry revealed cell spreading over the gel-cast membrane surfaces, characterized by trapezoidal morphology, distinct rounded nuclei, and well-aligned actin filaments. However, groups with higher ceramic loading expressed significantly higher levels of osteogenic markers relative to pure PLA membranes. Rule of mixtures and finite element models indicated an increase in theoretical mechanical strength with an increase in β-TCP concentration.</p><p><strong>Conclusion: </strong>This study potentiates the use of PLA/β-TCP composites in load bearing BTR applications and the ability to be used as customized patient-specific shape memory membranes in guided bone regeneration.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Temperature dependence of the protective effect of pressurized dissolution of xenon gas during cold storage of cells. 细胞冷藏期间加压溶解氙气的保护作用与温度有关。
IF 1 4区 医学 Q4 ENGINEERING, BIOMEDICAL Pub Date : 2024-09-05 DOI: 10.3233/BME-240105
Kenshi Mimura, Rina Sakai, Kazuhiro Yoshida, Masanobu Ujihira

Background: The preservation period afforded by cold storage of cells is short. However, the use of rare gases for cold storage as a means of extending the period of preservation would be highly beneficial.

Objective: To examine the effect of temperature on the protective effect of cold storage of cells using pressurized dissolution of xenon gas, with particular focus on the inhibition of substance transport by viscosity.

Methods: Human dermal fibroblast monolayers incubated in a culture dish for 48 h were used as a test sample, with culture medium used as a preservation solution. Samples were placed into a pressure-resistant vessel, which was pressurized with xenon gas at 0 or 0.5 MPa, and cells were stored at 0 to 5 °C for 18 h. Cell activity was evaluated by tetrazolium salt assay. The viscosity of the medium under pressurization at each storage temperature was estimated.

Results: The maximum protective effect against cell damage of cold storage with pressurized dissolution of xenon gas was observed at 4 °C. An increase in estimated viscosity by pressurization was correlated with increased cell activity at 4 °C.

Conclusion: Analysis of the temperature dependence of the protective effect against cell damage of cold storage with pressurized dissolution of xenon gas revealed that the most effective temperature is 4 °C. The data also suggest that increased viscosity due to pressurization plays a role in the protective effect.

背景:细胞冷藏的保存期很短。然而,使用稀有气体进行冷藏作为延长保存期的一种手段将大有裨益:研究温度对利用加压溶解氙气冷藏细胞的保护作用的影响,尤其关注粘度对物质运输的抑制作用。方法:以在培养皿中培养 48 小时的人真皮成纤维细胞单层为测试样本,培养基为保存液。将样品放入耐压容器中,用 0 或 0.5 兆帕的氙气对容器加压,细胞在 0 至 5 °C 下保存 18 小时。对每个储存温度下加压培养基的粘度进行了估算:结果:氙气加压溶解冷藏对细胞损伤的最大保护作用出现在 4 °C。在 4 °C时,加压估计粘度的增加与细胞活性的增加相关:分析加压溶解氙气冷藏对细胞损伤的保护作用与温度的关系发现,最有效的温度是 4 °C。数据还表明,加压导致的粘度增加在保护作用中起了一定作用。
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引用次数: 0
SIKVAV promotion proliferation of vascular endothelial cells and related mechanisms. SIKVAV 促进血管内皮细胞增殖及其相关机制。
IF 1 4区 医学 Q4 ENGINEERING, BIOMEDICAL Pub Date : 2024-08-29 DOI: 10.3233/BME-240018
Xionglin Chen, Jie Zhang, Xiaoming Cao, He Jiang, Zhiren Wu, Zi du Zeng, Chen Jiang, Hui Chen

Background: Vascular endothelial injury, a key factor in diabetic foot ulcers (DFUs) pathogenesis, is linked to the impaired proliferation and migration of vascular endothelial cells, modulated by hypoxia-inducible factor, growth factors, and inflammatory elements.

Objective: The present study assesses the role of SIKVAV (Ser-Ile-Lys-Val-Ala-Val), a peptide shown to enhance cell proliferation and migration, on mouse aortic endothelial cell (MAEC) and the corresponding molecular mechanisms.

Methods: MAEC were treated with SIKVAV at 0, 100, 200, 400, and 600 μg/mL for 0, 24, 48, and 72 h. Cell viability was tested using the CCK-8 assay. Proliferating cell nuclear antigen (PCNA), extracellular signal-regulated kinase 1/2 (ERK1/2), and protein kinase B (Akt) levels were measured by qRT-PCR and western blot.

Results: SIKVAV augmented PCNA mRNA expression and stimulated vascular endothelial cell proliferation in a concentration and time-dependent fashion. Furthermore, it amplified the expression of p-ERK1/2 and p-Akt, pivotal components of the mitogen-activated protein kinase (MAPK)/ERK1/2 and phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathways. The inhibition of these pathways suppressed PCNA mRNA expression, cell proliferation rate, and decreased p-ERK1/2 and p-Akt levels, highlighting SIKVAV's role in promoting vascular endothelial cell proliferation via these pathways.

Conclusion: The results of this study confirmed that SIKVAV grafted onto scaffolds can accelerate the proliferation of vascular endothelial cells for the therapy of skin wounds, and provide a theoretical basis for its application in ischemic disease as synthesized biomaterials scaffolds of tissue engineering.

背景:血管内皮损伤是糖尿病足溃疡(DFUs)发病机制中的一个关键因素,它与血管内皮细胞的增殖和迁移受损有关,并受缺氧诱导因子、生长因子和炎症因子的调节:本研究评估了 SIKVAV(Ser-Ile-Lys-Val-Ala-Val)对小鼠主动脉内皮细胞(MAEC)的作用及相应的分子机制:用 0、100、200、400 和 600 μg/mL 的 SIKVAV 处理小鼠主动脉内皮细胞 0、24、48 和 72 小时。增殖细胞核抗原(PCNA)、细胞外信号调节激酶1/2(ERK1/2)和蛋白激酶B(Akt)水平通过qRT-PCR和Western印迹进行检测:结果:SIKVAV能增强PCNA mRNA的表达,并以浓度和时间依赖性的方式刺激血管内皮细胞增殖。此外,它还增加了 p-ERK1/2 和 p-Akt 的表达,p-ERK1/2 和 p-Akt 是丝裂原活化蛋白激酶(MAPK)/ERK1/2 和磷脂酰肌醇-3-激酶(PI3K)/Akt 信号通路的关键成分。对这些通路的抑制抑制了 PCNA mRNA 的表达、细胞增殖率以及 p-ERK1/2 和 p-Akt 水平的降低,突出了 SIKVAV 通过这些通路促进血管内皮细胞增殖的作用:本研究结果证实,将 SIKVAV 移植到支架上可加速血管内皮细胞增殖,用于治疗皮肤创伤,并为其作为组织工程的合成生物材料支架应用于缺血性疾病提供了理论依据。
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引用次数: 0
Mesenchymal stem cells derived from human adipose tissue exhibit significantly higher chondrogenic differentiation potential compared to those from rats. 与大鼠的间充质干细胞相比,从人体脂肪组织中提取的间充质干细胞具有更高的软骨分化潜能。
IF 1 4区 医学 Q4 ENGINEERING, BIOMEDICAL Pub Date : 2024-08-29 DOI: 10.3233/BME-240062
Faisal Yaqoob, Muhammad Khizer Hayat, Muhammad Sharjeel Chaughtai, Sehrish Khan, Musa Bin Bashir

Background: Osteoarthritis is a prevalent joint disease affecting both humans and animals. It is characterized by articular cartilage degeneration and joint surface eburnation. Currently, no effective pharmacological treatment is available to restore the original function and structure of defective cartilage.

Objective: This study explores the potential of stem cell-based therapy in treating joint diseases involving cartilage degeneration, offering a promising avenue for future research and treatment. The primary aim was to compare the characteristics and, more importantly, the chondrogenic differentiation potential of human and rat adipose-derived mesenchymal stem cells (AD-MSCs).

Methods: Rat adipose tissue was collected from Sprague Dawley rats, while human adipose tissue was obtained in the form of lipoaspirate. The mesenchymal stem cells (MSCs) were then harvested using collagenase enzyme and subcultured. We meticulously evaluated and compared the cell morphology, percentage of cell viability, population doubling time, metabolic proliferation, and chondrogenic differentiation potential of MSCs harvested from both sources. Chondrogenic differentiation was induced at passage 3 using the 3D pellet culture method and assessed through histological and molecular analysis.

Results: The findings revealed that human and rat AD-MSCs were phenotypically identical, and an insignificant difference was found in cell morphology, percentage of cell viability, metabolic proliferation, and population doubling time. However, the chondrogenic differentiation potential of human AD-MSCs was evaluated as significantly higher than that of rat AD-MSCs.

Conclusion: The current study suggests that research regarding chondrogenic differentiation of rat AD-MSCs can be effectively translated to humans. This discovery is a significant contribution to the field of regenerative medicine and has the potential to advance our understanding of stem cell-based therapy for joint diseases.

背景:骨关节炎是一种影响人类和动物的常见关节疾病。其特点是关节软骨退化和关节表面烧伤。目前,还没有有效的药物治疗方法来恢复有缺陷软骨的原有功能和结构:本研究探讨了干细胞疗法在治疗涉及软骨退化的关节疾病方面的潜力,为未来的研究和治疗提供了一个前景广阔的途径。主要目的是比较人和大鼠脂肪间充质干细胞(AD-MSCs)的特征,更重要的是比较其软骨分化潜能:方法:大鼠脂肪组织取自 Sprague Dawley 大鼠,而人类脂肪组织取自脂肪抽吸物。然后使用胶原酶收获间充质干细胞(MSCs)并进行亚培养。我们对两种来源的间充质干细胞的细胞形态、细胞存活率、群体倍增时间、代谢增殖和软骨分化潜能进行了细致的评估和比较。使用三维颗粒培养法在第3周期诱导软骨分化,并通过组织学和分子分析进行评估:结果:研究结果表明,人和大鼠的 AD-MSCs 表型相同,在细胞形态、细胞存活率、代谢增殖和群体倍增时间方面差异不明显。然而,经评估,人 AD-MSCs 的软骨分化潜能明显高于大鼠 AD-MSCs:结论:目前的研究表明,有关大鼠 AD-MSCs 软骨分化的研究可以有效地应用于人类。这一发现是对再生医学领域的重大贡献,有可能促进我们对干细胞治疗关节疾病的理解。
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引用次数: 0
Accuracy of an image matching technique for assessing knee alignment during the stance phase of gait using single-plane anteroposterior radiography. 利用单平面正后方放射摄影评估步态阶段膝关节对齐情况的图像匹配技术的准确性。
IF 1 4区 医学 Q4 ENGINEERING, BIOMEDICAL Pub Date : 2024-08-16 DOI: 10.3233/BME-240059
Koichi Kobayashi, Makoto Sakamoto, Tatsuya Soeno, Takashi Sato

Background: Accurate measurement of bone alignment of the knee during walking provides ideal clinical information for diagnosis and treatment of knee joint disorders. To bring this ideal closer to clinical reality, we developed an image matching technique to measure the three-dimensional (3D) position of bones using anteroposterior radiography during the stance phase of treadmill walking.

Objective: To develop and validate an image matching method for evaluation of 3D knee alignment using anteroposterior radiography of artificial femoral and tibial bones.

Methods: The 3D position of each bone was recovered by minimizing the difference between the projected outline and the contour of the bone in the anteroposterior radiograph. The true value of the position was measured using a 3D coordinate measuring machine.

Results: The mean values ± standard deviation and root mean squares (RMS) of translation errors were within -1.6 ± 2.1 mm and 2.6 mm, respectively, for femur, and 2.1 ± 1.9 mm and 2.8 mm for tibia. The mean values ± standard deviation and RMS of errors in rotation were within 0.3 ± 0.7° and 0.7°, respectively, for femur, and -0.3 ± 0.9° and 0.9°, respectively, for tibia.

Conclusion: Our method is suitable for evaluating 3D knee alignment on anteroposterior radiography.

背景:精确测量行走时膝关节的骨骼排列为膝关节疾病的诊断和治疗提供了理想的临床信息。为了使这一理想更接近临床现实,我们开发了一种图像匹配技术,在跑步机行走的站立阶段使用前胸X光片测量骨骼的三维(3D)位置:目的:开发并验证一种图像匹配方法,利用人工股骨和胫骨的前正位放射摄影评估膝关节的三维对齐情况:方法:通过最小化投影轮廓与前后位X光片中骨骼轮廓之间的差异来恢复每块骨骼的三维位置。使用三维坐标测量机测量位置的真实值:股骨平移误差的平均值(± 标准差)和均方根(RMS)分别在-1.6± 2.1 毫米和 2.6 毫米以内,胫骨平移误差的平均值(± 标准差)和均方根(RMS)分别在 2.1± 1.9 毫米和 2.8 毫米以内。股骨旋转误差的平均值(±标准偏差)和均方根误差分别在 0.3 ± 0.7° 和 0.7° 以内,胫骨旋转误差的平均值(±标准偏差)和均方根误差分别在 -0.3 ± 0.9° 和 0.9° 以内:结论:我们的方法适用于在前正位X光片上评估膝关节的三维对齐情况。
{"title":"Accuracy of an image matching technique for assessing knee alignment during the stance phase of gait using single-plane anteroposterior radiography.","authors":"Koichi Kobayashi, Makoto Sakamoto, Tatsuya Soeno, Takashi Sato","doi":"10.3233/BME-240059","DOIUrl":"https://doi.org/10.3233/BME-240059","url":null,"abstract":"<p><strong>Background: </strong>Accurate measurement of bone alignment of the knee during walking provides ideal clinical information for diagnosis and treatment of knee joint disorders. To bring this ideal closer to clinical reality, we developed an image matching technique to measure the three-dimensional (3D) position of bones using anteroposterior radiography during the stance phase of treadmill walking.</p><p><strong>Objective: </strong>To develop and validate an image matching method for evaluation of 3D knee alignment using anteroposterior radiography of artificial femoral and tibial bones.</p><p><strong>Methods: </strong>The 3D position of each bone was recovered by minimizing the difference between the projected outline and the contour of the bone in the anteroposterior radiograph. The true value of the position was measured using a 3D coordinate measuring machine.</p><p><strong>Results: </strong>The mean values ± standard deviation and root mean squares (RMS) of translation errors were within -1.6 ± 2.1 mm and 2.6 mm, respectively, for femur, and 2.1 ± 1.9 mm and 2.8 mm for tibia. The mean values ± standard deviation and RMS of errors in rotation were within 0.3 ± 0.7° and 0.7°, respectively, for femur, and -0.3 ± 0.9° and 0.9°, respectively, for tibia.</p><p><strong>Conclusion: </strong>Our method is suitable for evaluating 3D knee alignment on anteroposterior radiography.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Testing the shock protection performance of Type I construction helmets using impactors of different masses 使用不同质量的冲击器测试 I 型建筑安全帽的防震性能
IF 1 4区 医学 Q3 Engineering Pub Date : 2024-04-23 DOI: 10.3233/bme-230173
John Z. Wu, Christopher S. Pan, Mahmood Ronaghi, Bryan M. Wimer
BACKGROUND:Wearing protective helmets is an important prevention strategy to reduce work-related traumatic brain injuries. The existing standardized testing systems are used for quality control and do not provide a quantitative measure of the helmet performance. OBJECTIVE:To analyze the failure characterizations of Type I industrial helmets and develop a generalized approach to quantify the shock absorption performance of Type I industrial helmets based on the existing standardized setups. METHODS:A representative basic Type I construction helmet model was selected for the study. Top impact tests were performed on the helmets at different drop heights using two different impactor masses (3.6 and 5.0 kg). RESULTS:When the helmets were impacted with potential impact energies smaller than the critical potential impact energy values, there was a consistent relationship between the peak impact force and the potential impact energy. When the helmets were impacted under potential impact energies greater than the critical potential impact energy values, the peak impact forces increased steeply with increasing potential impact energy. CONCLUSION:A concept of safety margin for construction helmets based on potential impact energy was introduced to quantify the helmets’ shock absorption performance. The proposed method will help helmet manufacturers improve their product quality.
背景:佩戴防护头盔是减少与工作有关的脑外伤的重要预防策略。现有的标准化测试系统用于质量控制,不能对安全帽的性能进行量化测量。目的:分析 I 型工业安全帽的失效特征,并在现有标准化设置的基础上开发一种通用方法来量化 I 型工业安全帽的减震性能。方法:研究选择了具有代表性的基本 I 型建筑安全帽模型。使用两种不同质量(3.6 千克和 5.0 千克)的冲击器,在不同下落高度对安全帽进行顶部冲击试验。结果:当安全帽受到小于临界潜在冲击能量值的冲击时,峰值冲击力与潜在冲击能量之间存在一致的关系。当头盔受到的潜在冲击能量大于临界潜在冲击能量值时,峰值冲击力随着潜在冲击能量的增加而急剧增加。结论:根据潜在冲击能量提出了建筑安全帽安全系数的概念,以量化安全帽的减震性能。所提出的方法将有助于安全帽制造商提高产品质量。
{"title":"Testing the shock protection performance of Type I construction helmets using impactors of different masses","authors":"John Z. Wu, Christopher S. Pan, Mahmood Ronaghi, Bryan M. Wimer","doi":"10.3233/bme-230173","DOIUrl":"https://doi.org/10.3233/bme-230173","url":null,"abstract":"BACKGROUND:Wearing protective helmets is an important prevention strategy to reduce work-related traumatic brain injuries. The existing standardized testing systems are used for quality control and do not provide a quantitative measure of the helmet performance. OBJECTIVE:To analyze the failure characterizations of Type I industrial helmets and develop a generalized approach to quantify the shock absorption performance of Type I industrial helmets based on the existing standardized setups. METHODS:A representative basic Type I construction helmet model was selected for the study. Top impact tests were performed on the helmets at different drop heights using two different impactor masses (3.6 and 5.0 kg). RESULTS:When the helmets were impacted with potential impact energies smaller than the critical potential impact energy values, there was a consistent relationship between the peak impact force and the potential impact energy. When the helmets were impacted under potential impact energies greater than the critical potential impact energy values, the peak impact forces increased steeply with increasing potential impact energy. CONCLUSION:A concept of safety margin for construction helmets based on potential impact energy was introduced to quantify the helmets’ shock absorption performance. The proposed method will help helmet manufacturers improve their product quality.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140799333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Bio-medical materials and engineering
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