Ritu Kumari, M Nasir Khan, Zubair Ahmad Parrey, Preedhi Kapoor, Bilal Ahmad Mir, Tuba Taziun, Parul Parihar, Gurmeen Rakhra
{"title":"Synergistic effects of hydrogen sulfide and nitric oxide in enhancing salt stress tolerance in cucumber seedlings.","authors":"Ritu Kumari, M Nasir Khan, Zubair Ahmad Parrey, Preedhi Kapoor, Bilal Ahmad Mir, Tuba Taziun, Parul Parihar, Gurmeen Rakhra","doi":"10.1111/ppl.70109","DOIUrl":null,"url":null,"abstract":"<p><p>Salinity stress poses a significant threat to plant growth and agricultural productivity, affecting millions of hectares of land worldwide. The adverse effects of salt toxicity, primarily caused by high levels of sodium chloride in soil and water, disrupt essential physiological processes in plants, leading to reduced yields and degraded soil quality. The present study thoroughly investigated the potential involvement of hydrogen sulphide (H<sub>2</sub>S) and nitric oxide (NO) in facilitating salt stress tolerance in cucumbers. In this investigation, NaHS (sodium hydrogen sulfide), which is the donor of H<sub>2</sub>S, and SNP (sodium nitroprusside), which is the donor of NO, were used as treatments for cucumber seedlings exposed to salt stress. Additionally, L-NAME (N-nitro-L-arginine: 100 μM) and cPTIO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide), which are inhibitors and scavengers of NO respectively, were used to verify the involvement of NO in the presence of salinity. NaHS and SNP supplementation significantly boosted fresh weight, dry weight, plant height, and chlorophyll content, promoting growth under salt stress. These treatments raised endogenous H<sub>2</sub>S and NO levels, upregulating antioxidative enzymes like SOD, CAT, APX, GR, GPX, and GSTs. This response reduced oxidative damages by lowering reactive oxygen species (ROS) and lipid peroxidation. The combined application of NaHS and SNP under salt stress offers a promising and cost-effective strategy to improve plant resilience to salinity, reduce oxidative stress, and ultimately enhance crop productivity. These findings provide important insights into the potential use of H<sub>2</sub>S and NO donors for sustaining agricultural production in saline environments, addressing a critical global challenge for food security.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 1","pages":"e70109"},"PeriodicalIF":5.4000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiologia plantarum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/ppl.70109","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Salinity stress poses a significant threat to plant growth and agricultural productivity, affecting millions of hectares of land worldwide. The adverse effects of salt toxicity, primarily caused by high levels of sodium chloride in soil and water, disrupt essential physiological processes in plants, leading to reduced yields and degraded soil quality. The present study thoroughly investigated the potential involvement of hydrogen sulphide (H2S) and nitric oxide (NO) in facilitating salt stress tolerance in cucumbers. In this investigation, NaHS (sodium hydrogen sulfide), which is the donor of H2S, and SNP (sodium nitroprusside), which is the donor of NO, were used as treatments for cucumber seedlings exposed to salt stress. Additionally, L-NAME (N-nitro-L-arginine: 100 μM) and cPTIO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide), which are inhibitors and scavengers of NO respectively, were used to verify the involvement of NO in the presence of salinity. NaHS and SNP supplementation significantly boosted fresh weight, dry weight, plant height, and chlorophyll content, promoting growth under salt stress. These treatments raised endogenous H2S and NO levels, upregulating antioxidative enzymes like SOD, CAT, APX, GR, GPX, and GSTs. This response reduced oxidative damages by lowering reactive oxygen species (ROS) and lipid peroxidation. The combined application of NaHS and SNP under salt stress offers a promising and cost-effective strategy to improve plant resilience to salinity, reduce oxidative stress, and ultimately enhance crop productivity. These findings provide important insights into the potential use of H2S and NO donors for sustaining agricultural production in saline environments, addressing a critical global challenge for food security.
期刊介绍:
Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.