Electrochemical assembly of 3-methoxycatechol and catechol on 1-Aminopyrene-functionalized carbon nanotubes for efficient NADH and glucose biosensing

Abstract

This study presents a novel electrochemical platform for efficient detection of NADH and glucose using multi-walled carbon nanotubes (MWCNTs) functionalized with 1-aminopyrene (1-AP) and catechol derivatives. The two-step functionalization process, involving π-π stacking of 1-AP followed by electrochemical assembly of catechol (Cat) and 3-methoxycatechol (3-MCat), enhances the electrocatalytic activity of the electrode surface. The optimized GC/MWCNT/1-AP/Cat electrode demonstrated excellent performance for NADH detection, achieving a detection limit of 1.98 μM at + 0.1 V. Further, the glucose biosensor, based on glucose dehydrogenase (GDH) immobilization, exhibited a detection limit of 0.166 mM with high stability and sensitivity. The proposed platform offers a robust and versatile solution for biosensing applications, showing significant potential in medical diagnostics and biochemical analysis.