SFP6 fluorescent probes for imaging SAM dynamics in living cells

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-02-21 DOI:10.1007/s00604-025-07039-7

Shuhui Zhang, Jinghui Li, Gengsheng Cao

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Abstract

A genetically encoded probe, SFP6 (S-adenosyl-L-methionine fluorescent probe), based on the principle of fluorescence resonance energy transfer (FRET) was developed. The SFP6 probe dynamically visualizes changes in S-adenosyl-L-methionine (SAM) levels in living cells with high spatiotemporal resolution. The results demonstrated that SFP6 exhibits high sensitivity to SAM, can be stably expressed in various mammalian cells, and has excellent biocompatibility. The probe accurately monitors SAM levels and detects changes caused by both endogenous and exogenous factors. In summary, we have developed a fluorescent probe that can monitor changes in SAM levels with single-cell and time resolution. Dynamic changes in SAM levels are linked to various methylation modifications in cells. Therefore, monitoring intracellular SAM concentrations offers the possibility to study physiological and biochemical processes in real-time, such as gene expression and metabolism, related to methylation modifications.

Graphical Abstract

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.