In vitro and in vivo evaluation of the trypanocidal activity of the acetone extract of lichen Usnea steineri and its chemical constituents.

IF 1.9 3区化学 Q3 CHEMISTRY, APPLIED Natural Product Research Pub Date : 2025-02-20 DOI:10.1080/14786419.2025.2469307

Luiz Antonio Ferreira, Daniele da Silva Ferreira, Marcos Gomide Tozatti, Marcelly Canuto de Almeida Silva, Milton Groppo, Lizandra Guidi Magalhães, Márcio Luis Andrade E Silva, Ana Helena Januário, Patrícia Mendonça Pauletti, Leonardo Bindelli Verly, Mário F C Santos, Wilson Roberto Cunha

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Abstract

More than 2.5 billion people live in endemic areas for neglected tropical diseases caused by the protozoan Trypanosoma cruzi. Which establishes chronic infections and increases public health spending. Thus, this paper describes the chemical study of Usnea steineri acetone extract and evaluations of trypanocidal activity in vitro and in vivo. Four chemical constituents from the acetone extract have been isolated: usnic acid, diffractaic acid, galbinic acid, and norstictic acid. The acetone extract of U. steineri revealed an IC₅₀ of 49.4 ± 1.5 µg/mL regarding the trypomastigote T. cruzi forms. The usnic acid revealed the biggest effectivity regarding the trypomastigote T. cruzi forms. The trypanocidal activity of the usnic acid regarding the amastigote forms revealed promising results, showing IC₅₀ of 67.0 ± 3.8 µM. The in vivo evaluation with the usnic acid revealed a decreased parasitemia at 40 mg/kg. Therefore, the results obtained open the perspectives for future studies aiming at its pharmacological application.

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.