In vitro and in vivo evaluation of the trypanocidal activity of the acetone extract of lichen Usnea steineri and its chemical constituents.

IF 1.6 3区化学 Q3 CHEMISTRY, APPLIED Natural Product Research Pub Date : 2025-02-20 DOI:10.1080/14786419.2025.2469307

Luiz Antonio Ferreira, Daniele da Silva Ferreira, Marcos Gomide Tozatti, Marcelly Canuto de Almeida Silva, Milton Groppo, Lizandra Guidi Magalhães, Márcio Luis Andrade E Silva, Ana Helena Januário, Patrícia Mendonça Pauletti, Leonardo Bindelli Verly, Mário F C Santos, Wilson Roberto Cunha

{"title":"In vitro and in vivo evaluation of the trypanocidal activity of the acetone extract of lichen Usnea steineri and its chemical constituents.","authors":"Luiz Antonio Ferreira, Daniele da Silva Ferreira, Marcos Gomide Tozatti, Marcelly Canuto de Almeida Silva, Milton Groppo, Lizandra Guidi Magalhães, Márcio Luis Andrade E Silva, Ana Helena Januário, Patrícia Mendonça Pauletti, Leonardo Bindelli Verly, Mário F C Santos, Wilson Roberto Cunha","doi":"10.1080/14786419.2025.2469307","DOIUrl":null,"url":null,"abstract":"More than 2.5 billion people live in endemic areas for neglected tropical diseases caused by the protozoan Trypanosoma cruzi. Which establishes chronic infections and increases public health spending. Thus, this paper describes the chemical study of Usnea steineri acetone extract and evaluations of trypanocidal activity in vitro and in vivo. Four chemical constituents from the acetone extract have been isolated: usnic acid, diffractaic acid, galbinic acid, and norstictic acid. The acetone extract of U. steineri revealed an IC50 of 49.4 ± 1.5 µg/mL regarding the trypomastigote T. cruzi forms. The usnic acid revealed the biggest effectivity regarding the trypomastigote T. cruzi forms. The trypanocidal activity of the usnic acid regarding the amastigote forms revealed promising results, showing IC50 of 67.0 ± 3.8 µM. The in vivo evaluation with the usnic acid revealed a decreased parasitemia at 40 mg/kg. Therefore, the results obtained open the perspectives for future studies aiming at its pharmacological application.","PeriodicalId":18990,"journal":{"name":"Natural Product Research","volume":" ","pages":"1-6"},"PeriodicalIF":1.6000,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Natural Product Research","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1080/14786419.2025.2469307","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}

引用次数: 0

Abstract

More than 2.5 billion people live in endemic areas for neglected tropical diseases caused by the protozoan Trypanosoma cruzi. Which establishes chronic infections and increases public health spending. Thus, this paper describes the chemical study of Usnea steineri acetone extract and evaluations of trypanocidal activity in vitro and in vivo. Four chemical constituents from the acetone extract have been isolated: usnic acid, diffractaic acid, galbinic acid, and norstictic acid. The acetone extract of U. steineri revealed an IC₅₀ of 49.4 ± 1.5 µg/mL regarding the trypomastigote T. cruzi forms. The usnic acid revealed the biggest effectivity regarding the trypomastigote T. cruzi forms. The trypanocidal activity of the usnic acid regarding the amastigote forms revealed promising results, showing IC₅₀ of 67.0 ± 3.8 µM. The in vivo evaluation with the usnic acid revealed a decreased parasitemia at 40 mg/kg. Therefore, the results obtained open the perspectives for future studies aiming at its pharmacological application.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

地衣丙酮提取物及其化学成分体外、体内杀锥虫活性评价。

超过 25 亿人生活在由原生动物克鲁斯锥虫引起的被忽视的热带疾病流行地区。这种疾病会造成慢性感染，增加公共卫生支出。因此，本文介绍了对甜叶菊丙酮提取物的化学研究以及体外和体内杀锥虫活性的评估。从丙酮萃取物中分离出了四种化学成分：琥珀酸（usnic acid）、衍生物酸（diffractaic acid）、高白藜芦酸（galbinic acid）和去甲藜芦酸（norstictic acid）。U. steineri 的丙酮提取物对胰母细胞瘤 T. cruzi 形态的 IC50 值为 49.4 ± 1.5 µg/mL。对于克鲁兹胰母细胞瘤来说，麝香草苷酸的作用最大。鼠李酸对变形体的杀胰活性显示出良好的结果，IC50 为 67.0 ± 3.8 µM。使用烟酸进行的体内评估显示，40 毫克/千克的剂量可降低寄生虫血症。因此，所获得的结果为今后研究其药理应用开辟了前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.