Genomic Characterization of Circulating Dengue Virus, Ethiopia, 2022–2023

IF 7.2 2区医学 Q1 IMMUNOLOGY Emerging Infectious Diseases Pub Date : 2025-02-21 DOI:10.3201/eid3103.240996

Adugna Abera, Houriiyah Tegally, Geremew Tasew, Eduan Wilkinson, Abraham Ali, Feyisa Regasa, Molalegne Bitew, Mahlet Belachew, Lucious Chabuka, Gaspary Mwanyika, Derek Tshiabuila, Jennifer Giandhari, Sureshnee Pillay, Jenicca Poogavanan, Monika Moir, Moritz U.G. Kraemer, Kamran Khan, Carmen Huber, Getachew Tollera, Tobias F. Rinke de Wit, Cheryl Baxter, Richard Lessells, Dawit Wolday, Dereje Beyene, Tulio de Oliveira

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Abstract

In Ethiopia, dengue virus (DENV) infections have been reported in several regions; however, little is known about the genetic diversity of circulating viruses. We conducted clinical surveillance of DENV during the 2023 nationwide outbreak in Ethiopia. We enrolled patients at 3 sentinel hospital sites. Using reverse transcription PCR, we screened serum samples for 3 arboviruses and then serotyped and whole-genome sequenced DENV-positive samples. We detected DENV-1 and DENV-3 serotypes. Phylogenetic analysis identified 1 transmission cluster for DENV-1 (genotype III major lineage A) and 2 clusters for DENV-3 (genotype III major lineage B). The first DENV-3 cluster was closely related to an isolate from a 2023 dengue outbreak in Italy; the second cluster was related to isolates from India. Co-circulation of DENV-1 and DENV-3 in Ethiopia highlights the potential for severe dengue. Intensified surveillance and coordinated public health responses are needed to address the threat of severe dengue outbreaks.

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在埃塞俄比亚，多个地区都有登革热病毒（DENV）感染的报道；然而，人们对流行病毒的遗传多样性知之甚少。在 2023 年埃塞俄比亚全国疫情爆发期间，我们对登革热病毒进行了临床监测。我们在 3 个哨点医院登记了患者。我们使用反转录 PCR 对血清样本进行了 3 种虫媒病毒筛查，然后对 DENV 阳性样本进行了血清分型和全基因组测序。我们检测到了 DENV-1 和 DENV-3 血清型。系统发生学分析确定了一个DENV-1传播集群（基因型III主系A）和两个DENV-3集群（基因型III主系B）。第一个DENV-3集群与2023年意大利登革热疫情中的一个分离株密切相关；第二个集群与印度的分离株有关。DENV-1和DENV-3在埃塞俄比亚的共同流行突显了严重登革热的潜在危险。为应对严重登革热疫情的威胁，需要加强监测和协调公共卫生应对措施。

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来源期刊

Emerging Infectious Diseases 医学-传染病学

CiteScore

17.30

自引率

1.70%

发文量

505

审稿时长

1 months

期刊介绍： Emerging Infectious Diseases is a monthly open access journal published by the Centers for Disease Control and Prevention. The primary goal of this peer-reviewed journal is to advance the global recognition of both new and reemerging infectious diseases, while also enhancing our understanding of the underlying factors that contribute to disease emergence, prevention, and elimination. Targeted towards professionals in the field of infectious diseases and related sciences, the journal encourages diverse contributions from experts in academic research, industry, clinical practice, public health, as well as specialists in economics, social sciences, and other relevant disciplines. By fostering a collaborative approach, Emerging Infectious Diseases aims to facilitate interdisciplinary dialogue and address the multifaceted challenges posed by infectious diseases.