Chemical Constituents from Achyranthes bidentata and Their Antihyperuricemic Acid Activities

Abstract

One new coumaronochromone named 5,7,2′-trihydroxy-6-methoxycoumaronochromone (1), and six known compounds named benzyl-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside (2), chikusetsusaponin V (3), crotonine (4), ayamenin A (5), achyranthoside A (6), 5-hydroxypyridine-2-carboxylic acid methyl ester (7), were obtained from Achyranthes bidentata. The antihyperuricemic acid activities of these isolated compounds (1–7) were assayed by an HK-2 cell model induced by adenosine combined with xanthine oxidase. The contents of uric acid produced by the HK-2 cell model were determined by HPLC analysis. As the results show, compound 3 was the most effective and was almost in line with the positive drug of febuxostat, whereas compounds 1, 4, 5, and 6 showed moderate antihyperuricemic acid activities at a concentration of 50.0 μM.