Genomic surveillance and evolution of Getah virus.

IF 5.5 2区医学 Q1 VIROLOGY Virus Evolution Pub Date : 2025-02-04 eCollection Date: 2025-01-01 DOI:10.1093/ve/veaf007

Jiaqi Shen, Shaogui Liu, Shiqiang Liu, Shiyi Shen, Mingkai Lei, Qian Xu, Wentao Li, Qigai He, Xiaojuan Xu, Xuwang Cai

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Abstract

Getah virus (GETV), a member of the Alphaviruses, has spread widely and is expanding its host range worldwide, posing a serious threat to public health safety and the farming industry. However, genetic monitoring of GETV is inadequate, and its evolution and transmission remain unclear. This study employed reverse transcription-polymerase chain reaction to screen pig tissue samples for the presence of GETV. Subsequent steps included DNA sequencing, phylogenetic analysis, and selection pressure assessments to elucidate the evolutionary history and transmission patterns of the virus. A total of 1382 samples were examined, with a positivity rate of 4.12% (95% confidence interval: 3.07%-5.17%) from 2022 to 2023. Subsequently, seven GETV strains were isolated and identified. A phylogenetic tree was constructed, which showed that all seven strains belonged to Group III. Phylodynamic analysis revealed that GETV evolved rapidly. Additionally, eight amino acid sites within the GETV E2 protein were identified as being under positive selection. These data provide insight into the epidemiology and evolution of GETV.

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来源期刊

Virus Evolution Immunology and Microbiology-Microbiology

CiteScore

10.50

自引率

5.70%

发文量

108

审稿时长

14 weeks

期刊介绍： Virus Evolution is a new Open Access journal focusing on the long-term evolution of viruses, viruses as a model system for studying evolutionary processes, viral molecular epidemiology and environmental virology. The aim of the journal is to provide a forum for original research papers, reviews, commentaries and a venue for in-depth discussion on the topics relevant to virus evolution.