Metabolomics and Transcriptomics Reveal the Role of the Terpene Biosynthetic Pathway in the Mechanism of Insect Resistance in Solanum habrochaites

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of Agricultural and Food Chemistry Pub Date : 2025-02-25 DOI:10.1021/acs.jafc.4c10397

Yudan Wang, Ruihua Gao, Tingting Gu, Xinzhi Li, Meiliang Wang, Aoxue Wang, Youwen Qiu

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Abstract

Terpenes are a large variety of natural organic compounds that can enhance the resistance of plants to phytophagous insects through induction. In this study, differential expression genes and metabolites of Alice Craig (AC) and Solanum habrochaite (SH) were screened and analyzed by transcriptomics and metabolomics. The results show that terpene biosynthesis is one of the most crucial secondary metabolic pathways in plants. SH significantly accumulates more terpenes than AC by up regulating the expression of relevant genes. It is worth noting that virus-induced SlHDR silencing not only reduces the expression of downstream genes (SlTPS3, SlFPP, and SlGGPPS) in the terpene biosynthesis pathway, but also significantly affects the synthesis of related terpenoids, there by reducing the insect resistance of tomatoes. The results will be beneficial for understanding the synthesis mechanism of terpenoids in tomatoes and supply new genetic resources for the development of insect-resistant tomatoes.

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代谢组学和转录组学揭示了萜烯生物合成途径在哈马藻抗虫机制中的作用

萜烯是一种种类繁多的天然有机化合物，可以通过诱导增强植物对植食性昆虫的抗性。本研究对Alice Craig （AC）和Solanum habrochaite （SH）的差异表达基因和代谢物进行了转录组学和代谢组学筛选和分析。结果表明，萜烯生物合成是植物体内最重要的次生代谢途径之一。SH通过上调相关基因的表达，显著积累了比AC更多的萜烯。值得注意的是，病毒诱导的SlHDR沉默不仅降低了萜类生物合成途径中下游基因（SlTPS3、SlFPP和SlGGPPS）的表达，而且显著影响了相关萜类的合成，从而降低了番茄的抗虫性。研究结果将有助于了解番茄中萜类化合物的合成机理，并为开发抗虫番茄提供新的遗传资源。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.