Exploring the antibiotic potential of copper carbonate nanoparticles, wound healing, and glucose-lowering effects in diabetic albino mice

Abstract

Bio-Nanoscience is an emerging field that integrates nanotechnology with biological systems to revolutionize medicine, agriculture, and environmental sustainability through innovative and targeted solutions. The aim of this study was to synthesize copper carbonate nanoparticles and to investigate their antibacterial, wound healing, and glucose-lowering properties. Nanoparticles (NPs) were Synthesized through chemical reduction method and confirmed by using SEM, XRD, and FTIR. Characterization revealed that the nanoparticles had an average size of 55 ± 16 nm, exhibited a crystalline structure, and were free of impurities. Antibacterial tests demonstrated enhanced inhibition zones for Pseudomonas spp., S. aureus, and other bacterial strains, with the largest zone of inhibition observed at 12 mg/ml, measuring 18.5 ± 1.05 mm for Pseudomonas spp. In wound healing activity in diabetic mice observations revealed a complete wound closure in NPs treated mice by day 14 as compared to the control group (96.10 % wound closure). Nanoparticle administration (oral) also significantly reduced glucose levels in diabetic mice after 15 days in the experimental period, whereas fasting glucose levels reduced from 398.00 ± 6.16 to 116.67 ± 12.47 mg/dl. The docking studies of copper carbonate nanoparticles (NPs) with proteins involved in wound healing, including Antileukoproteinase (−2.7 kcal/mol), Casein (−2.5 kcal/mol), Collagen (−2.9 kcal/mol), Lysozyme (−2.8 kcal/mol), and Phospholipase (−3.9 kcal/mol), revealed significant binding affinities, suggesting potential applications in enhancing wound healing processes. Therefore, the copper carbonate nanoparticles demonstrate strong antibacterial properties and show promising effects on wound healing, along with blood glucose-lowering activity. These findings suggest their potential in biomedical applications, particularly for treating diabetes and bacterial infections.