Evaluation of Paracentrotus lividus spines extract antioxidant, antidiabetic, anti-inflammatory, antimicrobial, and mechanistic anticancer: Insights into its composition using UPLC-ESI-MS-based metabolomic profiling

Abstract

Sea urchins are widely distributed in waters worldwide. The present study carried out the in vitro metabolomic bioactivity profiling using UPLCMS/MS of Paracentrotus lividus spines extract. Investigations were also conducted on molecular anticancer, anti-inflammatory, antidiabetic, antioxidant, and antibacterial properties. A comprehensive untargeted metabolic profiling of P. lividus spines extract resulted in the classification of more than 13 metabolites. Their metabolomic quantitative evaluations were assumed by measuring total phenolic, flavonoids, dihydroflavonol, sugar, and protein contents. The P. lividus spines extract exhibited powerful antioxidant capacity using DPPH*, ABTS⁺, reducing power, and phosphomolybdate assays. Moreover, P. lividus spines extract highly elucidated antidiabetic and anti-inflammatory activity by inhibiting α-amylase enzyme and protein denaturation. Further, the spines of the P. lividus exhibited significant antibacterial effects. Besides, extract from P. lividus spines showed a strong cytotoxic impact against a variety of HepG-2 and MCF-7 cancer cell lines. It was discovered that the P. lividus spines extract triggered cell cycle arrest in the sub-G0/G1 phase and suppressed the growth of cancer cells via suppressing mRNA of Akt/MAPK/Bcl-2/c-myc and protein expression of β-Catenin/ki-67. Conclusively, the extract derived from the spines of the sea urchin species P. lividus demonstrates significant potential for utilization in various pharmaceutical industries.