Therapeutic Potential of Cymbopogon citratus Hydroalcoholic Extract and Vitamin E Coadministration in Alleviating Complications From Testicular Ischemia–Reperfusion-Induced Injury in Rats

Abstract

Background: Impaired circulation caused by testicular torsion can lead to infertility and testicular damage. Factors such as increased production of free radicals and inflammatory cytokines as well as damage to blood vessels may play a role in the development of postreperfusion injury. Compounds containing antioxidants may inhibit or reduce complications caused by testicular torsion by neutralizing free radical activity.

Objectives: This study investigates the evaluation of the therapeutic efficacy of Cymbopogon citratus hydroalcoholic extract (CCHE) and vitamin E coadministration against complications caused by testicular torsion.

Methods: Thirty male Wistar rats were allocated into five groups of six: (1) negative control; (2) positive control (sham); (3) intraperitoneally (CCHE); (4) vitamin E intramuscularly; and (5) coadministration of CCHE and vitamin E. Testicular ischemia was induced in groups 2, 3, 4, and 5. Subsequently, the treatment was performed on groups 3, 4, and 5 for 3 weeks daily. Sections of testes were collected on day 21. Superoxide dismutase (SOD) and catalase (CAT) were assessed by real-time PCR, and their activities were analyzed. Moreover, a histopathological evaluation was also performed.

Results: Coadministration of CCHE and vitamin E significantly reduced the complications caused by testicular torsion.