The Combined Toxic Effects of Polystyrene Microplastics and Arsenate on Lettuce Under Hydroponic Conditions.

Abstract

The combined pollution of microplastics (MPs) and arsenic (As) has gradually been recognized as a global environmental problem, which calls for detailed investigation of the synergistic toxic effects of MPs and As on plants and their mechanisms. Therefore, the interaction between polystyrene microplastics (PS-MPs) and arsenate (AsO₄^3-) (in the following text, it is abbreviated as As(V)) and its toxic effects on lettuce were investigated in this study. Firstly, chemisorption was identified as the main mechanism between PS-MPs and As(V) by the analysis of adsorption kinetics, adsorption thermodynamics, and Fourier transform infrared spectroscopy (FTIR). At the same time, the addition of As(V) promoted the penetration of PS-MPs through the continuous endodermal region of the Casparis strip. Furthermore, compared with the CK group, it was found that the co-addition of As(V) exacerbated the lowering effect of PS-MPs on the pH value of the rhizosphere environment and the inhibitory effect on root growth. In the P20V10 group, the pH decreased by 33.0%. Compared to the CK group, P20, P20V1, and P20V10 decreased the chlorophyll content by 68.45% (16 SPAD units), 71.37% (17.73 SPAD units), and 61.74% (15.36 SPAD units) and the root length by 19.31% (4.18 cm), 50.72% (10.98 cm), and 47.90% (10.37 cm) in lettuce. P5V10 and P20V10 increased CAT content by 153.54% (33.22 U·(mgprol)^-1) and 182.68% ((38.2 U·(mgprol)^-1)), Ca by 31.27% and 37.68%, and Zn by 41.85% and 41.85%, but the presence of As(V) reduced Na by 22.85% (P5V1) and 49.95% (P5V10). The co-exposure significantly affected the physiological and biochemical indicators as well as the nutritional quality of the lettuce. Finally, the metabolomic analysis of the lettuce leaves showed that combined pollution with PS-MPs and As(V) affected the metabolic pathways of the tricarboxylic acid cycle (TCA cycle), sulfur metabolism, and pyruvate metabolism. This study provides data for pollution management measures for co-exposure to PS-MPs and As(V).