Stable isotope labelling and gene expression analysis reveal dynamic nitrogen-supply mechanisms for rapid growth of Moso bamboo

IF 8.7 1区 农林科学 Q1 Agricultural and Biological Sciences Horticulture Research Pub Date : 2025-02-25 DOI:10.1093/hr/uhaf062
Junbo Zhang, Man Shi, Chenglei Zhu, Kebin Yang, Quan Li, Xiaoming Song, Zhimin Gao, Tingting Cao, Dezheng Zhu, Xinzhang Song
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Abstract

Rapid growth of Moso bamboo (Phyllostachys edulis) shoots (offspring ramet) is primarily fuelled by nitrogen (N) derived from parent ramet and absorbed by rhizome roots. However, the extent to which each N source supports the growth of offspring ramet and the underlying molecular mechanisms of N transport remain unclear. Here, clonal fragments consisting of a parent ramet, an offspring ramet, and an interconnected rhizome were established in a Moso bamboo forest. Additionally, 15N isotope tracing and transcriptome profiling were conducted concurrently to quantify the N contribution from the parent ramet and rhizome roots to the offspring ramet, and to reveal the molecular mechanisms underlying N transport during rapid growth (i.e., early, peak, branching, and leafing stages). The N acquisition strategy of offspring ramet shifted from being primarily provided by the parent ramet (72.53%) during early stage to being predominantly absorbed by rhizome roots (69.85%) during the leafing stage. Approximately equal N contributions (45.82–54.18%) from the parent ramet and rhizome roots were observed during peak and branching stages. PeAAP29123 was identified as a key gene for N transport, being most closely correlated with 15N content. Biomolecular assays demonstrated that PeHDZ23987 could activate the expression of PeAAP29123 via two types of HD-motifs. Overexpression of PeHDZ23987 and PeAAP29123 significantly enhanced N starvation tolerance in transgenic rice with significantly improved N uptake efficiency. Our findings clarify the pattern and mechanisms of N supply for the rapid growth of Moso bamboo offspring ramet and provide transcriptomic evidence for long-distance N transport between clonal ramets.
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来源期刊
Horticulture Research
Horticulture Research Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
11.20
自引率
6.90%
发文量
367
审稿时长
20 weeks
期刊介绍: Horticulture Research, an open access journal affiliated with Nanjing Agricultural University, has achieved the prestigious ranking of number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. As a leading publication in the field, the journal is dedicated to disseminating original research articles, comprehensive reviews, insightful perspectives, thought-provoking comments, and valuable correspondence articles and letters to the editor. Its scope encompasses all vital aspects of horticultural plants and disciplines, such as biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.
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