A novel peptide encoded by a rice circular RNA confers broad-spectrum disease resistance in rice plants

IF 8.1 1区生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2025-02-25 DOI:10.1111/nph.70018

Xin Pan, Sipei Xu, Gehui Cao, Siping Chen, Tong Zhang, Burton B. Yang, Guohui Zhou, Xin Yang

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Abstract

Circular RNAs (circRNAs) are a significant class of endogenous RNAs that exert crucial biological functions in human and animal systems, but little is currently understood regarding their roles in plants.
Here, we identified a circRNA originating from the back-splicing of exon 4 and exon 5 of a rice gene, OsWRKY9, and named it circ-WRKY9. It is upregulated in rice stripe mosaic virus (RSMV)-infected rice plants. Notably, circ-WRKY9 contains two open reading frames with an internal ribosome entry site. We found that circ-WRKY9 encoded a peptide of 88 amino acids (aa) and named it WRKY9-88aa.
Overexpression of WRKY9-88aa suppresses RSMV infection in rice plants, with increased reactive oxygen species production. Furthermore, WRKY9-88aa enhances resistance to blast disease and bacterial leaf blight, suggesting its potential to provide broad-spectrum disease resistance.
Our findings provide the first evidence of a peptide encoded by a circRNA in planta and highlight its potential application to control a wide spectrum of plant diseases.

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水稻环状 RNA 编码的一种新型多肽赋予水稻植株广谱抗病性

环状rna （circRNAs）是一类重要的内源性rna，在人类和动物系统中发挥重要的生物学功能，但目前对其在植物中的作用知之甚少。在这里，我们发现了一个起源于水稻基因OsWRKY9的外显子4和外显子5的反向剪接的circRNA，并将其命名为circ-WRKY9。它在水稻条纹花叶病毒（RSMV）感染的水稻植株中表达上调。值得注意的是，circ-WRKY9包含两个具有内部核糖体进入位点的开放阅读框。我们发现circ-WRKY9编码88个氨基酸（aa）的肽，并将其命名为WRKY9-88aa。WRKY9-88aa过表达可以抑制水稻RSMV感染，并增加活性氧的产生。此外，WRKY9-88aa增强了稻瘟病和细菌性叶枯病的抗性，表明其具有广谱抗病性的潜力。我们的发现首次证明了植物中存在环状rna编码的肽，并强调了其在控制多种植物疾病方面的潜在应用。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.