A core-satellite self-assembled SERS aptasensor used for ultrasensitive detection of AFB1

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-02-26 DOI:10.1007/s00604-025-07040-0

Danni Sun, Tao Liu, Yiran Yao, Dezhao Kong, Chang Liu, Hua Ye, Qi Zhang, Shijie Li, Yaqi Li, Qiaoqiao Shi

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Abstract

A surface-enhanced Raman scattering (SERS) aptasensor was developed using gold nanostars (Au NSs) and Fe₃O₄@Au nanoparticles (NPs) for the highly sensitive detection of aflatoxin B₁ (AFB₁). Au NSs were modified by the Raman reporter 4-aminothiophenol (PATP) and then coupled with cDNA to act as the capture probes (Au NSs@PATP-cDNA). Fe₃O₄@Au NPs were modified with the AFB₁ aptamer (AFB₁ Apt) and used as signal probes (Fe₃O₄@Au NPs-AFB₁ Apt). The SERS peak of PATP at 1078 cm⁻¹ was used for quantitative analysis. When the core-satellite nanostructures (Fe₃O₄@Au NPs-AFB₁ Apt/cDNA-Au NSs@PATP) were self-assembled by oligonucleotide hybridization, the SERS intensity was significantly enhanced. When AFB₁ was present, AFB₁ Apt specifically binds to AFB₁, causing the Fe₃O₄@Au NPs-AFB₁ Apt and Au NSs@PATP-cDNA to dissociate, resulting in a decrease in the SERS intensity measured after magnetic separation. Under optimal conditions, the limit of detection (LOD) of AFB₁ can be reduced to 0.24 pg/mL. This is attributed to the high affinity of AFB₁ Apt, excellent magnetic separation characteristics, and multiple SERS hotspots. The assay has been validated to perform well in recovery and accuracy by evaluating spiked samples (rice, corn, and wheat) and positive samples (corn, brown rice, and wheat).

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一种用于AFB1超灵敏检测的核心-卫星自组装SERS适配体传感器

采用金纳米星（Au NSs）和Fe3O4@Au纳米粒子（NPs）制备了一种表面增强拉曼散射（SERS）传感器，用于黄曲霉毒素B1 （AFB1）的高灵敏度检测。通过拉曼报告蛋白4-氨基噻吩（PATP）修饰Au NSs，然后与cDNA偶联作为捕获探针（Au NSs@PATP-cDNA）。Fe3O4@Au NPs用AFB1适配体（AFB1 Apt）修饰，用作信号探针（Fe3O4@Au NPs-AFB1 Apt）。利用1078 cm−1处的PATP SERS峰进行定量分析。当核心-卫星纳米结构（Fe3O4@Au NPs-AFB1 Apt/cDNA-Au NSs@PATP）通过寡核苷酸杂交自组装时，SERS强度显著增强。当AFB1存在时，AFB1 Apt特异性结合AFB1，导致Fe3O4@Au NPs-AFB1 Apt与Au NSs@PATP-cDNA解离，导致磁分离后测得的SERS强度降低。在最佳条件下，AFB1的检出限（LOD）可降至0.24 pg/mL。这归因于AFB1 Apt的高亲和力、优异的磁分离特性和多个SERS热点。通过评价加标样品（水稻、玉米和小麦）和阳性样品（玉米、糙米和小麦），验证了该方法具有良好的回收率和准确性。图形抽象

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tris (2-carboxyethyl) phosphine

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trisodium citrate

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silver nitrate

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gold (III) chloride trihydrate

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4-aminophenylthiol

来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.