Magnetic rod-shaped Mn-based MOF as a multi-functional and recyclable platform for dual-mode ratiometric-based nitrite detection

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-02-27 DOI:10.1007/s00604-025-07054-8

Sameera Sh. Mohammed Ameen, Faisal Algethami, Khalid M. Omer

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Abstract

The development is shown of rod-shaped manganese-based metal–organic frameworks (Mn-MOFs) as hot- and cold-adapted oxidase-like nanozymes, with strong magnetic properties. These Mn-MOFs enable highly sensitive detection of nitrite ions, utilizing both convenient colorimetric ratio analysis and a visual instrument-free-based approach compatible with smartphone-based detection. The Mn-MOF showed multi-functional activity, such as cold/hot-adapted and magnetic oxidase-like activity, catalyzing the oxidation of chromogenic substrates 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxidized TMB (oxTMB). Mn-MOF shows high oxidase activity with Vmax of 1.39 × 10⁻⁸ M/s and Km of 0.068 mM for TMB oxidation. Nitrite ions further react with oxTMB to form a yellow color via diazotization resulting in the ratiometric change in absorbance (A₆₅₂/A₄₆₁). The color ratio is also quantified through the naked eye and/or smartphone app by analyzing RGB values, providing a rapid, portable, and cost-effective method for on-site detection. When applying Mn-MOF for smartphone-based nitrite detection, it performs excellent detection, with a linear range of 5.0–55.0 µM and a limit of detection of 0.18 µM, superior to most of the oxidase nanozyme-based nitrite sensing platforms. The detection platforms develop sensing probes using a reusable nanozyme that enables highly sensitive and selective detection of nitrite, featuring a broad linear range and a low limit of detection.

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磁性棒状锰基MOF作为一种多功能可回收的双模比测亚硝酸盐检测平台

棒状锰基金属有机框架（Mn-MOFs）是一种具有强磁性的热适应性和冷适应性的类氧化酶纳米酶。这些mn - mof能够高度灵敏地检测亚硝酸盐离子，利用方便的比色比分析和基于视觉仪器的方法，与基于智能手机的检测兼容。Mn-MOF具有冷/热适应性和磁性氧化酶样活性，可催化显色底物3,3 ',5,5 ' -四甲基联苯胺（TMB）氧化为蓝氧化TMB （oxTMB）。Mn-MOF对TMB氧化表现出较高的氧化酶活性，Vmax为1.39 × 10−8 M/s， Km为0.068 mM。亚硝酸盐离子进一步与oxTMB反应，通过重氮作用形成黄色，导致吸光度的比例变化（A652/A461）。色比也可以通过肉眼和/或智能手机应用程序通过分析RGB值来量化，为现场检测提供了一种快速，便携式和经济高效的方法。将Mn-MOF用于基于智能手机的亚硝酸盐检测时，其检测效果非常好，线性范围为5.0-55.0µM，检测限为0.18µM，优于大多数基于氧化酶纳米酶的亚硝酸盐检测平台。检测平台使用可重复使用的纳米酶开发传感探针，可以对亚硝酸盐进行高灵敏度和选择性检测，具有宽线性范围和低检测限。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.