A Deep Dive Into the Potential Antiaging Effects of Colla Corii Asini on the Skin: A Comparative Study

IF 2.3 4区医学 Q2 DERMATOLOGY Journal of Cosmetic Dermatology Pub Date : 2025-02-27 DOI:10.1111/jocd.70080

Fan Wu, Xiao Xie, Wenwen Ru, Xiaofei Guo, Lingfeng Meng, Jianling Zhang, Xiuling Liu, Yao Pan

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Abstract

Background

Skin aging results in skin barrier damage and skin wrinkles. Oral Colla Corii Asini (CCA) can show significant antiaging effects. However, few studies have investigated the skin's antiaging effects of topical CCA. Here, we investigated and compared the effects of CCA crushed (CCAC) and its enzymatic digest, CCA oligopeptide (CCAO), on skin barrier function and wrinkle formation.

Methods

CCAO and CCAC were prepared by enzymatic digestion and pulverization. HaCaT cells and HSF cells were selected as in vitro cell models. Cytotoxicity and cell proliferation were detected by MTT assay; scratch assay was used to evaluate the effect on cell migration; quantitative real-time PCR (qRT-PCR), immunofluorescence, and ELISA were used to determine the changes in expression of the barrier-related molecules (AQP3, FLG, and LOR) and antiwrinkle firming-related molecules (COL-1, COL-3, ELN, and MMP-1).

Results

MTT assay showed no significant cytotoxicity of CCAO and CCAC at < 2.5 mg/mL. After treating the cells with safe doses of CCAO and CCAC, HaCaT cell proliferation and migration were significantly enhanced, and there was no difference between the two effects. The expression levels of AQP3, FLG, and LOR were all significantly increased compared with the NC group, in which CCAO mainly promoted FLG and LOR expression, while CCAC mainly promoted the expression of AQP3. At the same time, CCAO and CCAC also significantly upregulated the expression levels of COL-1, COL-3 and ELN, and downregulated MMP-1 expression in HSF cells, and the effect of CCAO was more significant.

Conclusion

Topical application of CCA improves the skin barrier, increases collagen levels, and enhances skin elasticity, thus slowing down skin aging. CCAO is more easily absorbed and utilized by the skin due to its small molecular weight, which gives a better effect.

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来源期刊

Journal of Cosmetic Dermatology DERMATOLOGY-

CiteScore

4.30

自引率

13.00%

发文量

818

审稿时长

>12 weeks

期刊介绍： The Journal of Cosmetic Dermatology publishes high quality, peer-reviewed articles on all aspects of cosmetic dermatology with the aim to foster the highest standards of patient care in cosmetic dermatology. Published quarterly, the Journal of Cosmetic Dermatology facilitates continuing professional development and provides a forum for the exchange of scientific research and innovative techniques. The scope of coverage includes, but will not be limited to: healthy skin; skin maintenance; ageing skin; photodamage and photoprotection; rejuvenation; biochemistry, endocrinology and neuroimmunology of healthy skin; imaging; skin measurement; quality of life; skin types; sensitive skin; rosacea and acne; sebum; sweat; fat; phlebology; hair conservation, restoration and removal; nails and nail surgery; pigment; psychological and medicolegal issues; retinoids; cosmetic chemistry; dermopharmacy; cosmeceuticals; toiletries; striae; cellulite; cosmetic dermatological surgery; blepharoplasty; liposuction; surgical complications; botulinum; fillers, peels and dermabrasion; local and tumescent anaesthesia; electrosurgery; lasers, including laser physics, laser research and safety, vascular lasers, pigment lasers, hair removal lasers, tattoo removal lasers, resurfacing lasers, dermal remodelling lasers and laser complications.