Orthotopic Transplantation of Native and Cryopreserved Ovarian Tissue in Day-Old Geese.

Abstract

As an alternative method for preserving female genetic material, the cryopreservation and orthotopic transplantation of day-old gonadal tissue have become well-developed techniques in a few poultry species. The aim of the present study was to apply these methods for the first time to domestic geese. Cryopreservation was accomplished using the previously developed vitrification technique. Native and frozen/thawed White Hungarian geese ovarian tissue was transplanted onto Grey Landes geese recipients. Both donors and recipients were less than 24 h old. The surgical protocol previously used in chickens needed to be modified regarding anaesthesia; ketamine, xylazine, and midazolam were administered partly intramuscularly and partly intravenously. After sexual maturation, the Grey Landes recipients were paired with White Hungarian ganders, and eggs were collected and hatched individually. The origin of the offspring was determined by phenotype- and microsatellite markers. Donor-derived offspring were obtained from native and frozen/thawed ovarian tissue transplantation, which had not been published earlier. The ratio of donor-derived progeny producing layers was 40% and 58% in native and frozen/thawed gonadal tissue transplantation, respectively. The highest rate of donor-originated progeny was 78.9%. Consequently, gonadal tissue transplantation appears to be a suitable method for preserving female genetic material in domestic geese; the technique is already implemented in Hungarian gene bank practice.