Synthesis of Eu, Sm and Dy metal-organic framework nanosheets based on pyridyl carboxylic acid and their cytotoxic mechanism in vitro

IF 4 2区化学 Q2 CHEMISTRY, PHYSICAL Journal of Molecular Structure Pub Date : 2025-02-25 DOI:10.1016/j.molstruc.2025.141853

Xin Luo , Siying Chong , Yang Li , Shuangyan Wu , Yaguang Sun , Mingchang Zhu , Ying Zhang , Chiyu Sun

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Abstract

The rational design of Ln-MOF nanosheets (NSs) with tunable and ultrathin structure is promising in tumor therapy. A series of Ln-DPIA1–3 (Ln = Eu, Sm and Dy) MOF NSs were prepared by the hydrothermal method. Ln-DPIA1–3 MOF NSs were characterized by SC-XRD, PXRD, NMR, HRMS and FT-IR spectra, TG and EA. The morphology and element distribution of complexes was observed by SEM, TEM and EDS. The malignant biological behavior of Ln-DPIA1–3 MOF NSs against BxPC-3 cells was studied by MTT assay, flow cytometry and fluorescence imaging. Ln-DPIA1–3 MOF NSs exhibited moderate cytotoxicity against BxPC-3 cells, especially for Eu-DPIA with IC₅₀ value of 10.08 μg/mL. Cytotoxic mechanism of the complexes was investigated through competitive binding assay, DNA binding affinity determination, DNA cleavage and molecular docking analysis. In addition, ADME parameter for Ln-DPIA1–3 MOF NSs were simulated. As a result, the complexes inserted to DNA instead of EtBr and strengthened DNA cleavage. Moreover, the DNA binding constant for Eu-DPIA was highest among Ln-DPIA1–3 MOF NSs, and Eu-DPIA interacted with target DNA through multiple hydrogen bonds.

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来源期刊

Journal of Molecular Structure 化学-物理化学

CiteScore

7.10

自引率

15.80%

发文量

2384

审稿时长

45 days

期刊介绍： The Journal of Molecular Structure is dedicated to the publication of full-length articles and review papers, providing important new structural information on all types of chemical species including: • Stable and unstable molecules in all types of environments (vapour, molecular beam, liquid, solution, liquid crystal, solid state, matrix-isolated, surface-absorbed etc.) • Chemical intermediates • Molecules in excited states • Biological molecules • Polymers. The methods used may include any combination of spectroscopic and non-spectroscopic techniques, for example: • Infrared spectroscopy (mid, far, near) • Raman spectroscopy and non-linear Raman methods (CARS, etc.) • Electronic absorption spectroscopy • Optical rotatory dispersion and circular dichroism • Fluorescence and phosphorescence techniques • Electron spectroscopies (PES, XPS), EXAFS, etc. • Microwave spectroscopy • Electron diffraction • NMR and ESR spectroscopies • Mössbauer spectroscopy • X-ray crystallography • Charge Density Analyses • Computational Studies (supplementing experimental methods) We encourage publications combining theoretical and experimental approaches. The structural insights gained by the studies should be correlated with the properties, activity and/ or reactivity of the molecule under investigation and the relevance of this molecule and its implications should be discussed.