Synthesis of silver nanoparticles by Sedum adenotrichum extract and evaluating their antioxidant potential in albino mice

Abstract

Nanoparticles (NPs) are colloidal particles ranging in size from 1 to 100 nm. They consist of a variety of various materials including metals, polymers, lipids and phospholipids. NPs exhibit unique physical and chemical properties due to the size and high surface area to volume ratio relative to their bulk material. Silver is one of the oldest metals used as an antimicrobial, antioxidant, and antiseptic. Silver was traditionally used in bulk, but nanotechnology has allowed us to save our precious metal resources. We can use a minute dose of the same metal with the best results in the form of nanoparticles. Green synthesis methods are being processed to harvest all the benefits of silver and avoid its inimical effects. Plant extracts or microbes are used as reducing and stabilizing agents. This study also attempts to synthesize silver nanoparticles using Sedum adenotrichum extract and evaluate their antioxidant activity. High potential liquid chromatography (HPLC) was performed for elemental analysis and Sedum adenotrichum synthesized silver nanoparticles (S.AgNPs) were characterized by UV–Vis spectroscopy and FTIR. Synthesized nanoparticles' in vitro antioxidant potential were evaluated. For in vivo antioxidant analysis, twenty-five albino mice were randomly divided into five groups. Each treatment group received doses at 20, 30 and 40 mg per body weight according to their group. DPPH and FRP activities of S.AgNPs 24.03 % and 0.21 % were greater than chemically synthesized silver nanoparticles (C.AgNPs) 7.25 % and 0.11 % respectively. In vivo, the antioxidant activity of S.AgNPs was also significantly different (p < 0.05) when compared with C.AgNPs. In conclusion, S.AgNPs are eco-friendly, efficient, and more powerful antioxidants.