Journal of Drug Delivery Science and Technology最新文献

Chrysin-loaded Soluplus-TPGS mixed micelles: Optimization, characterization and anticancer activity against hepatocellular carcinoma cell line 金黄素负载的 Soluplus-TPGS 混合胶束：针对肝癌细胞系的优化、表征和抗癌活性

IF 4.5 3区医学 Q1 PHARMACOLOGY & PHARMACY

Journal of Drug Delivery Science and Technology

Pub Date : 2024-11-04 DOI: 10.1016/j.jddst.2024.106371

Chrysin is a natural flavonoid found in various plants, and it has shown potential therapeutic benefits such as anti-inflammatory, antioxidant, and anticancer properties. However, its clinical application is limited due to poor solubility and low bioavailability. Mixed micelles can help overcome these problems. This study focuses on preparation of Chrysin-loaded mixed micelles with the help of solvent diffusion evaporation method. Soluplus and TPGS were the main components of the formulation, and their proportions were optimized with the help of the central composite design matrix. Thirteen batches were constructed to optimize the mixed micelle formulation based on different Soluplus: Chrysin ratios and TPGS percentages. Response surface methodology and various models were employed to analyze micellar size, size distribution, encapsulation efficiency, and drug loading. The optimized formulation, CHR-MM1, exhibited a particle size of 132.2 ± 7.9 nm, encapsulation efficiency of 98.01 ± 2.27 %, and a zeta potential of −2.10 mV. TEM images revealed that the micelles were spherical in shape. Characterization studies, including FTIR and X-ray diffraction, confirmed the successful encapsulation of Chrysin in an amorphous form within the mixed micelles. The in vitro release studies demonstrated a sustained release behavior in both acidic and neutral pH conditions. The Korsmeyer-Peppas model best described the drug release kinetics. Cellular uptake studies using fluorescence microscopy revealed enhanced internalization of the mixed micelles into Hep G2 cells. Moreover, MTT assays indicated a concentration- and time-dependent cytotoxicity of Chrysin-loaded mixed micelles against Hep G2 cells, outperforming free Chrysin. The flow cytometry analysis results suggested an enhanced apoptotic activity of Chrysin in the mixed micelles as compared to free drug. This study provides a promising strategy for improving the solubility, cellular uptake, and cytotoxicity of Chrysin. Therefore, our results point to the potential of Chrysin-loaded mixed micelles to serve as a therapeutic option for hepatocellular carcinoma.

菊黄素是一种存在于多种植物中的天然类黄酮，具有潜在的治疗功效，如抗炎、抗氧化和抗癌。然而，由于溶解性差和生物利用率低，它的临床应用受到了限制。混合胶束有助于克服这些问题。本研究主要利用溶剂扩散蒸发法制备金黄素负载混合胶束。配方的主要成分是 Soluplus 和 TPGS，并借助中心复合设计矩阵对它们的比例进行了优化。根据不同的 Soluplus（茄红素）和 TPGS（菊黄素）比例和 TPGS 百分比，构建了 13 个批次来优化混合胶束配方：Chrysin 的比例和 TPGS 的百分比来优化混合胶束配方。采用响应面方法和各种模型来分析胶束尺寸、尺寸分布、封装效率和药物负载。优化配方 CHR-MM1 的粒径为 132.2 ± 7.9 nm，封装效率为 98.01 ± 2.27 %，zeta 电位为 -2.10 mV。TEM 图像显示胶束呈球形。包括傅立叶变换红外光谱和 X 射线衍射在内的表征研究证实，在混合胶束中以无定形形式成功地包封了 Chrysin。体外释放研究表明，在酸性和中性 pH 条件下都能实现持续释放。Korsmeyer-Peppas 模型最好地描述了药物释放动力学。利用荧光显微镜进行的细胞摄取研究表明，混合胶束在 Hep G2 细胞中的内化增强。此外，MTT 试验表明，负载了 Chrysin 的混合胶束对 Hep G2 细胞具有浓度和时间依赖性细胞毒性，优于游离 Chrysin。流式细胞术分析结果表明，与游离药物相比，混合胶束中的金丝桃苷具有更强的凋亡活性。这项研究为改善金黄素的溶解度、细胞吸收和细胞毒性提供了一种有前景的策略。因此，我们的研究结果表明，添加了 Chrysin 的混合胶束有可能成为治疗肝细胞癌的一种选择。

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引用次数: 0

Composite hydrogel contact lens systems for naringenin: A comparison of drug loading and release behaviour in drug carrier systems 柚皮苷复合水凝胶隐形眼镜系统：药物载体系统中药物负载和释放行为的比较

IF 4.5 3区医学 Q1 PHARMACOLOGY & PHARMACY

Journal of Drug Delivery Science and Technology

Pub Date : 2024-11-04 DOI: 10.1016/j.jddst.2024.106375

引用次数: 0

Tobacco stalk nanofibrillated cellulose: An eco-friendly binder on fluidized bed granulation 烟草茎秆纳米纤维素：流化床造粒中的环保型粘合剂

IF 4.5 3区医学 Q1 PHARMACOLOGY & PHARMACY

Journal of Drug Delivery Science and Technology

Pub Date : 2024-11-02 DOI: 10.1016/j.jddst.2024.106370

The search for new excipients that provide formulation alternatives over existing ones and that are derived from natural resources has advanced over the years. Emerging from this scenario, an important alternative to traditional excipients is nanofibrillated cellulose (NFC), which is generally obtained from agricultural wastes and is a cellulose-rich material. Most of the time, the nanofibers are presented in aqueous dispersions that form a highly entangled network of fibrous particles that exhibit gel-like behavior. Therefore, the present work aimed to explore the gel-like behavior of NFC from tobacco stalks to evaluate it as a binder in a fluid bed granulation process for further application as a pharmaceutical ingredient. The granulation process was carried out using top spray mode with an inlet air temperature of 80 °C, airflow between 9 and 11 mm³ h⁻¹, and atomizing air pressure of 0.7 bar. Povidone (PVP) was used as a standard binder for comparison purposes. The binder systems were sprayed on the powder blend of theophylline and maltodextrin at a feed rate of 3.05 mL⋅min⁻¹. Granule properties, such as yield, moisture content, morphology, label-free Raman imaging, particle size distribution, drug content, and flow properties, were evaluated. The process using NFC provided granules with excellent drug uniformity, low moisture content (<2 %), high yield (>92 %), and passable flow properties. Their behavior was comparable to that of granules prepared with the classical PVP binder system. Therefore, this study opens a perspective for the reuse of NFC from tobacco stalk, an agricultural waste, as an alternative binder for the granulation process, contributing to environmental sustainability.

多年来，人们一直在寻找可替代现有配方、从自然资源中提取的新辅料。在这种情况下，纳米纤维素（NFC）成为传统辅料的一个重要替代品，这种材料通常从农业废弃物中提取，是一种富含纤维素的材料。大多数情况下，纳米纤维以水分散体的形式出现，形成高度纠缠的纤维颗粒网络，表现出凝胶状行为。因此，本研究旨在探索从烟草茎秆中提取的 NFC 的凝胶状行为，以评估其在流化床造粒工艺中作为粘合剂进一步应用于药物成分的情况。制粒过程采用顶部喷雾模式，进气温度为 80 °C，气流介于 9 至 11 mm3 h-1 之间，雾化空气压力为 0.7 巴。聚维酮（PVP）作为标准粘合剂用于比较。以 3.05 mL⋅min-1 的进料速度将粘合剂系统喷洒在茶碱和麦芽糊精的混合粉末上。对颗粒特性进行了评估，如产量、水分含量、形态、无标记拉曼成像、粒度分布、药物含量和流动特性。采用 NFC 工艺制得的颗粒具有极佳的药物均匀性、低水分含量（2%）、高产率（92%）和良好的流动性。其性能与使用传统 PVP 粘合剂体系制备的颗粒剂相当。因此，这项研究为重新利用烟草茎秆（一种农业废弃物）中的 NFC 作为制粒过程中的替代粘合剂开辟了前景，有助于环境的可持续发展。

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引用次数: 0

A novel solvent-extruded tacrolimus-eluting suture for attenuated inflammation and scarring in skin repair 一种新型溶剂挤出他克莫司洗脱缝合线，可减轻皮肤修复中的炎症和瘢痕形成

IF 4.5 3区医学 Q1 PHARMACOLOGY & PHARMACY

Journal of Drug Delivery Science and Technology

Pub Date : 2024-11-02 DOI: 10.1016/j.jddst.2024.106374

This study aimed to design an absorbable suture that locally delivers the immunosuppressive agent, tacrolimus (FK506), to attenuate scar tissue formation by modulating inflammation. As the suture degrades, it releases FK506 directly into the repair site. The poly (l-lactide-co-caprolactone) (PLLA-PCL) polymer and 0.2 % wt FK506 were co-extruded using a solvent extrusion technique to generate the drug-eluting sutures. The properties of the generated sutures were assessed regarding FK506 release in vitro, wound closure in an in vivo murine model, murine skin histology for structural and inflammatory markers, and mechanical strength of both sutures and healed murine skin. The generated suture consistently released FK506 at ≤10 ng/day rates. Following annealing, the sutures retained 60 % of their anticipated tensile strength, exhibiting enhanced ductility. The histological examination and in vivo murine study indicated that wounds treated with FK506-eluting PLLA-PCL sutures produced a thinner epidermal layer, enhanced integrin β4 expression, increased keratinocyte presence, attenuated inflammation, and reduced scarring, demonstrating healed tissue improvements compared with those treated with the placebo, poly (glycolic-co-caprolactone) (PGCL), sutures. Mechanical evaluations of healed tissue indicated that drug-PLLA-PCL sutures did not reduce the mechanical strength of the skin compared to placebo PGCL sutures. The FK506-eluting PLLA-PCL sutures show promising results in advancing wound healing by offering targeted drug delivery. This innovative method can improve wound healing and provide a streamlined approach to scar management. Continued research, optimization, and broad-scale studies are needed to refine the manufacturing process and ensure that the properties of the suture meet clinical requirements.

本研究旨在设计一种可吸收缝合线，在局部释放免疫抑制剂他克莫司（FK506），通过调节炎症来减轻瘢痕组织的形成。随着缝合线的降解，FK506 会直接释放到修复部位。聚（l-乳酸-共己内酯）（PLLA-PCL）聚合物和 0.2% wt 的 FK506 采用溶剂挤出技术共挤制成药物洗脱缝合线。对所生成缝合线的性能进行了评估，包括体外的 FK506 释放情况、体内小鼠模型的伤口闭合情况、小鼠皮肤组织学的结构和炎症标记以及缝合线和愈合的小鼠皮肤的机械强度。生成的缝合线能以≤10 纳克/天的速率持续释放 FK506。退火后，缝合线保留了 60% 的预期拉伸强度，并显示出更强的延展性。组织学检查和体内小鼠研究表明，与使用安慰剂聚（乙醇-共聚己内酯）（PGCL）缝合线处理的伤口相比，使用 FK506 洗脱 PLLA-PCL 缝合线处理的伤口表皮层变薄，整合素 β4 表达增强，角质形成细胞增多，炎症减轻，瘢痕减少，表明愈合组织有所改善。对愈合组织的机械评估表明，与安慰剂PGCL缝合线相比，药物-PLLA-PCL缝合线不会降低皮肤的机械强度。FK506洗脱PLLA-PCL缝合线通过提供靶向给药，在促进伤口愈合方面显示出良好的效果。这种创新方法可以改善伤口愈合，并提供一种简化的疤痕管理方法。为了完善生产工艺，确保缝合线的性能符合临床要求，还需要继续进行研究、优化和大规模研究。

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引用次数: 0

Nanostructured silver vanadate gel: Evaluation of physicochemical, mechanical, and antibiofilm properties against a five-species oral model 纳米结构钒酸银凝胶：针对五种口腔模型的理化、机械和抗生物膜特性评估

IF 4.5 3区医学 Q1 PHARMACOLOGY & PHARMACY

Journal of Drug Delivery Science and Technology

Pub Date : 2024-11-01 DOI: 10.1016/j.jddst.2024.106366

The aim of this study was to develop a gel with nanostructured silver vanadate decorated with silver nanoparticles (β-AgVO₃) and to evaluate its physicochemical, mechanical, and antibiofilm properties against a five-species oral model composed of Candida albicans, Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, and Pseudomonas aeruginosa. The formulations were prepared with 0 % w/v (G0), 0.02 % w/v (G1), 0.05 % w/v (G2), and 0.12 % w/v (G3) β-AgVO₃. The physicochemical properties of pH (n = 5), viscosity (n = 5), spreadability (n = 10) and syringability (n = 10) were evaluated, as well as the mechanical properties of hardness, cohesiveness, compressibility, elasticity, and adhesiveness using the texture profile assay (TPA) (n = 5) and ex vivo mucoadhesion (n = 5). The antibiofilm effect was assessed by counting colony-forming units (CFU/mL) (n = 8), metabolic activity (XTT) (n = 8), and scanning electron microscopy (SEM) (n = 1), using 0.12 % chlorhexidine gel (CG) as a positive control and G0 as a negative control. All groups demonstrated a neutral pH. G2 and G3 exhibited reduced viscosity and syringeability, along with increased spreadability. The incorporation of β-AgVO₃ did not impact the gel's elasticity or cohesiveness but led to a decrease in hardness, compressibility, and adhesiveness. Ex vivo mucoadhesion was not altered by the addition of the nanomaterial. G3 showed a higher microbial reduction compared to GC and G0. It was concluded that the formulations with β-AgVO₃ showed compatible physicochemical and mechanical properties for application and maintenance in the oral cavity. G3 showed superior antimicrobial effect against all microorganisms compared to GC and G0.

本研究的目的是开发一种用银纳米粒子（β-AgVO3）装饰的纳米结构钒酸银凝胶，并评估其针对由白色念珠菌、金黄色葡萄球菌、大肠杆菌、粪肠球菌和铜绿假单胞菌组成的五种口腔模型的理化、机械和抗生物膜特性。制备的制剂分别含有 0 % w/v (G0)、0.02 % w/v (G1)、0.05 % w/v (G2) 和 0.12 % w/v (G3) β-AgVO3。评估了 pH 值（5 个）、粘度（5 个）、铺展性（10 个）和可注射性（10 个）等理化特性，以及硬度、内聚性、可压缩性、弹性和粘附性等机械特性（采用纹理轮廓测定法（TPA），5 个）和体内外粘附性（5 个）。以 0.12 % 洗必泰凝胶（CG）为阳性对照，G0 为阴性对照，通过菌落形成单位计数（CFU/mL）（n = 8）、代谢活性（XTT）（n = 8）和扫描电子显微镜（SEM）（n = 1）来评估抗生物膜效果。所有组的 pH 值均为中性。G2 和 G3 组的粘度和可注射性均有所降低，但铺展性有所提高。β-AgVO3的加入不会影响凝胶的弹性或粘合性，但会导致硬度、可压缩性和粘合性下降。体内外粘附性未因添加纳米材料而改变。与 GC 和 G0 相比，G3 的微生物减少率更高。结论是，添加了 β-AgVO3 的制剂具有兼容的物理化学和机械性能，适合在口腔中应用和维护。与 GC 和 G0 相比，G3 对所有微生物都有较好的抗菌效果。

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引用次数: 0

Construction and evaluation of transdermal delivery system of terbinafine-loaded plant exosomes for the treatment of cutaneous fungal infections 构建和评估用于治疗皮肤真菌感染的特比萘芬负载型植物外泌体透皮给药系统

IF 4.5 3区医学 Q1 PHARMACOLOGY & PHARMACY

Journal of Drug Delivery Science and Technology

Pub Date : 2024-11-01 DOI: 10.1016/j.jddst.2024.106365

Local transdermal of antifungal agents is a problem that limits the effectiveness of clinical treatment of dermatomycosis. In this study, cucumber-derived plant extracellular vesicles (PEV) were extracted and loaded with the antifungal drug terbinafine (TBF) to form TBF-PEV, which was applied locally to the site of skin fungal infection to improve the transdermal transport capacity of TBF and the antifungal effect in vitro and in vivo. Chemical enhancer and physical enhancer methods were replaced by low immunogenicity and non-irritating PEV. TBF-PEV caused obvious curling and wrinkling in the skin, induced changes in the distribution of skin lipids, and changed the structure and content of skin lipids and proteins, thereby increasing the transdermal efficiency of TBF. The local drug concentration was increased, and the antifungal effect was improved in vitro and in vivo. It was first found that PEV inhibited the growth of Candida albicans with high efflux pump expression. In general, this paper has certain significance in local transdermal treatment of fungal skin infections and reversal of fungal resistance.

抗真菌药物的局部透皮是限制皮霉菌病临床治疗效果的一个问题。本研究提取黄瓜衍生的植物细胞外囊泡（PEV），载入抗真菌药物特比萘芬（TBF），形成TBF-PEV，局部应用于皮肤真菌感染部位，以提高TBF的透皮转运能力和体外、体内的抗真菌效果。低免疫原性和无刺激性的 PEV 取代了化学增强剂和物理增强剂方法。TBF-PEV 使皮肤明显卷曲起皱，引起皮肤脂质分布变化，改变了皮肤脂质和蛋白质的结构和含量，从而提高了 TBF 的透皮效率。提高了局部药物浓度，改善了体外和体内的抗真菌效果。首次发现 PEV 可抑制高外排泵表达的白色念珠菌的生长。总之，本文在局部透皮治疗皮肤真菌感染和逆转真菌耐药性方面具有一定的借鉴意义。

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引用次数: 0

Corrigendum to “Fabrication and characterization of dissolving microneedles for transdermal delivery of hypocrellin A” [J. Drug Deliv. Sci. Technol. 95 (May 2024) 105594] 用于透皮给药hypocrellin A的溶解微针的制作和特性"[J. Drug Deliv.

IF 4.5 3区医学 Q1 PHARMACOLOGY & PHARMACY

Journal of Drug Delivery Science and Technology

Pub Date : 2024-11-01 DOI: 10.1016/j.jddst.2024.106216

引用次数: 0

Engineering PSMA-targeted nanoparticles co-encapsulating mitoxantrone and indocyanine green for precise combinatory therapy in prostate cancer 设计 PSMA 靶向纳米粒子，共同封装米托蒽醌和吲哚菁绿，用于前列腺癌的精确联合治疗

IF 4.5 3区医学 Q1 PHARMACOLOGY & PHARMACY

Journal of Drug Delivery Science and Technology

Pub Date : 2024-11-01 DOI: 10.1016/j.jddst.2024.106369

Prostate cancer is the 2^nd most common cancer in men worldwide. Chemotherapeutic treatment of prostate cancer with mitoxantrone (MTX) has limited efficacy due to severe side effects in which cardiotoxicity and myelosuppression are the two major causes of its dose-limiting toxicity. This study aimed to obtain a poly (lactic-co-glycolic acid) (PLGA) nanoparticle that can precisely deliver MTX to the prostate cancer cells overexpressing the Prostate-specific membrane antigen (PSMA) receptor-sparing healthy tissues and co-loading Indocyanine green (ICG) as a fluorescent photothermal/photodynamic agent for precise combinatory therapy in prostate cancer. The biocompatible polymer PLGA was covalently modified with the peptide of sequence (WQPDTAHHWATL) to actively target the PSMA receptor. Factors like the peptide-to-polymer ratio or the peptide's orientation during the polymer's chemical modification were investigated to enhance the active targeting of the nanoparticles (NPs). NPs were characterised using dynamic light scattering, scanning electron microscopy, and UV–vis spectroscopy to determine their morphological and colloidal properties and optimal MTX and ICG encapsulation efficiency. Quantitative FACS analysis of LNCaP and PC-3 cells incubated with Nile Red-labelled non-targeted PLGA or PLGA-PSMA targeted NPs was assessed to identify the best formulation that bound selectively to PSMA. The orientation of the peptide conjugated to the polymer, which has the C-terminal end of the peptide sequence accessible for interaction with the cell receptor, maximises the targeting capacity of the system. Photothermal experiments using 808 nm near-infrared laser irradiation were conducted, and cytotoxicity was assessed using the resazurin viability assay. Remarkably, our results confirmed the safety and efficacy of a targeted and activatable therapy using polymeric NPs functionalised with the peptide and co-loaded with MTX and ICG. This pioneer nanosystem opens new perspectives for exploring advanced targeted delivery in prostate cancer. It offers a straightforward methodology for functionalising drug delivery systems with bioactive peptides that can be applied to different types of cancer.

前列腺癌是全球第二大男性常见癌症。米托蒽醌（MTX）对前列腺癌的化疗疗效有限，原因在于其严重的副作用，其中心脏毒性和骨髓抑制是导致剂量限制性毒性的两个主要原因。本研究旨在获得一种聚（乳酸-共聚-乙酸）（PLGA）纳米粒子，它能将 MTX 精准地输送到过度表达前列腺特异性膜抗原（PSMA）受体的前列腺癌细胞，而不损伤健康组织，同时还能添加靛氰绿（ICG）作为荧光光热/光动力药剂，用于前列腺癌的精确联合治疗。生物相容性聚合物 PLGA 与序列为 (WQPDTAHHWATL) 的多肽共价修饰，可主动靶向 PSMA 受体。为了增强纳米粒子（NPs）的主动靶向性，研究人员对聚合物化学修饰过程中的肽与聚合物比例或肽的取向等因素进行了研究。利用动态光散射、扫描电子显微镜和紫外-可见光谱对 NPs 进行了表征，以确定其形态和胶体特性以及 MTX 和 ICG 的最佳封装效率。评估了与尼罗河红标记的非靶向 PLGA 或 PLGA-PSMA 靶向 NPs 培养的 LNCaP 和 PC-3 细胞的定量 FACS 分析，以确定选择性结合 PSMA 的最佳配方。肽与聚合物共轭的取向使肽序列的 C 端可以与细胞受体相互作用，从而最大限度地提高了系统的靶向能力。我们使用 808 纳米近红外激光照射进行了光热实验，并使用resazurin 细胞活力测定法评估了细胞毒性。值得注意的是，我们的研究结果证实了使用多肽功能化聚合物 NPs 并共同负载 MTX 和 ICG 的靶向可激活疗法的安全性和有效性。这一开创性的纳米系统为探索前列腺癌的先进靶向给药开辟了新的前景。它为生物活性肽功能化给药系统提供了一种简单易行的方法，可用于不同类型的癌症。

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引用次数: 0

Folic acid conjugated sodium alginate based redox responsive smart functional microgels as a potential targeted anticancer drug carrier 叶酸共轭海藻酸钠氧化还原反应智能功能微凝胶作为潜在的靶向抗癌药物载体

IF 4.5 3区医学 Q1 PHARMACOLOGY & PHARMACY

Journal of Drug Delivery Science and Technology

Pub Date : 2024-11-01 DOI: 10.1016/j.jddst.2024.106372

In this study, we have fabricated a sodium alginate-based redox-responsive, and cancer cell-targeted specific microgel using a water in oil (w/o) mini emulsion process and characterized it accordingly. Initially, we synthesized folic acid (FA)-grafted sodium alginate (SA), a biocompatible polysaccharide, followed by the preparation of a semi-interpenetrating polymer network (semi-IPN) microgel by crosslinking polyacrylamide (PAAm) with a disulfide crosslinker. The presence of disulfide linkages in the microgel formulation accelerated the release of the anti-cancer drug Doxorubicin (DOX) in the reducing domain of cancer cells (in vitro) (cumulative drug release amount 11 ± 3 %). Folic acid was incorporated into the microgels to enhance its targeting towards cancer cells due to the presence of the folate receptor on cancer cells. MTT (3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay showed an IC₅₀ of ∼30 μg/mL of the DOX-loaded microgels towards breast cancer cells (MDA-MB-231) and a nontoxic behaviour towards normal mouse fibroblast cells (L929). FACS (Fluorescence-Activated Cell Sorting) and cell uptake studies showed significant cell apoptosis upon application of the drug-loaded microgels. In our opinion, this type of microgel can be a potential drug delivery vector for cancer treatment.

在本研究中，我们采用油包水型（w/o）微型乳液工艺制备了一种基于海藻酸钠的氧化还原反应性和癌细胞靶向特异性微凝胶，并对其进行了相应的表征。首先，我们合成了叶酸（FA）接枝的海藻酸钠（SA）（一种生物相容性多糖），然后用二硫化物交联剂交联聚丙烯酰胺（PAAm），制备出半互穿聚合物网络（semi-IPN）微凝胶。微凝胶配方中二硫键的存在加速了抗癌药物多柔比星（DOX）在癌细胞还原域（体外）的释放（累积药物释放量为 11 ± 3 %）。由于癌细胞上存在叶酸受体，因此在微凝胶中加入叶酸可增强其对癌细胞的靶向性。MTT（3-(4,5-二甲基噻唑基-2)-2, 5-二苯基溴化四氮唑）测定显示，负载 DOX 的微凝胶对乳腺癌细胞（MDA-MB-231）的 IC50 值为 30 μg/mL，而对正常小鼠成纤维细胞（L929）则无毒性。荧光激活细胞分拣（FACS）和细胞摄取研究表明，使用药物负载微凝胶后，细胞凋亡显著。我们认为，这种微凝胶可以成为一种潜在的癌症治疗药物递送载体。

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引用次数: 0

Corrigendum to “Synergistic strategies for enhanced liver cancer therapy with sorafenib/resveratrol PEGylated liposomes in vitro and in vivo” [J. Drug Deliv. Sci. Technol. 96 (2024) 105703] 对 "索拉非尼/瑞弗瑞 PEG 化脂质体在体外和体内增强肝癌治疗的协同策略 "的更正 [J. Drug Deliv.

IF 4.5 3区医学 Q1 PHARMACOLOGY & PHARMACY

Journal of Drug Delivery Science and Technology

Pub Date : 2024-11-01 DOI: 10.1016/j.jddst.2024.106301

引用次数: 0