Application of Eosin Y as a Probe for Spectroscopic Determination of Aripiprazole in Human Plasma

Abstract

This work presents the development of new, rapid, and sensitive spectrophotometric and spectrofluorimetric methods for the determination of Aripiprazole in different dosage forms and human plasma. Both methods are based on the formation of an ion-pair complex between Eosin Y and the studied drug in an acidic medium. In the spectrophotometric method, the absorbance of the formed complex was estimated at 551 nm, showing a linear range from 1.0 to 13.0 μg mL⁻¹ with detection and quantification limits of 0.20 and 0.60 μg mL⁻¹, respectively. The spectrofluorimetric method measured the fluorescence emission of the formed complex at 545 nm upon excitation at 255 nm. This method exhibited a good linear range from 0.1 to 2.0 μg mL⁻¹ with detection and quantification limits of 0.03 and 0.09 μg mL⁻¹, respectively. The developed techniques offer several benefits, such as simplicity, cost-effectiveness, and high sensitivity. The proposed methods were validated in accordance with ICH guidelines, permitting precise analysis of the studied drug in its tablets and spiked human plasma samples with a high percent recovery. Moreover, the eco-friendly characteristics of the proposed methods were evaluated using two greenness assessment tools: AGREE and GAPI.