Deciphering immune tolerance in allogeneic pig pregnancy

Abstract

Embryo transfer (ET) has transformed swine biotechnology, enabling genetic advancements and disease control. However, its success remains inconsistent, partly due to immune-mediated challenges at the maternal-fetal interface. This study explores the immunological environment of hemi-allogeneic pregnancies (via artificial insemination, AI) versus allogeneic pregnancies (via ET) in pigs during the critical implantation phase. Sows were categorized into groups based on pregnancy type and fetal counts, reflecting varying outcomes. Endometrial immune cell populations, including T lymphocytes, regulatory T cells (T_regs), natural killer (NK) cells, and macrophages, were analyzed using immunohistochemistry. Notably, allogeneic pregnancies with poor outcomes displayed elevated NK cell and macrophage infiltration alongside reduced T_reg presence, contributing to a pro-inflammatory environment. In contrast, allogeneic pregnancies with favorable outcomes exhibited immune profiles resembling hemi-allogeneic pregnancies, suggesting enhanced maternal-fetal tolerance. These findings underscore the immunological variability driving embryo survival rates in ET pregnancies and highlight key cellular targets for improving reproductive efficiency. Further research on immunomodulatory strategies is vital to optimize ET success in swine and other species.