{"title":"Allosteric modulation of protein–protein interactions in signal transduction with Nanobodies","authors":"Els Pardon , Alex Wohlkönig , Jan Steyaert","doi":"10.1016/j.sbi.2025.103022","DOIUrl":null,"url":null,"abstract":"<div><div>Nanobodies (Nbs), the variable domains of heavy-chain only antibodies that naturally occur in camelids, are exquisite molecular tools to stabilize dynamic proteins in unique functional conformations. Recent developments in Nb discovery allow to select allosteric Nbs that perturb the distribution of conformational ensembles of protein complexes that mediate signaling, leading to the allosteric modulation of the signals they transmit. Evidence is also accumulating that such conformational specific Nbs do not stabilize new conformational states but rather change the distribution of existing states to allosterically induce transitions, imprinted by the natural ligands of the system.</div></div>","PeriodicalId":10887,"journal":{"name":"Current opinion in structural biology","volume":"92 ","pages":"Article 103022"},"PeriodicalIF":6.1000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current opinion in structural biology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0959440X25000405","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Nanobodies (Nbs), the variable domains of heavy-chain only antibodies that naturally occur in camelids, are exquisite molecular tools to stabilize dynamic proteins in unique functional conformations. Recent developments in Nb discovery allow to select allosteric Nbs that perturb the distribution of conformational ensembles of protein complexes that mediate signaling, leading to the allosteric modulation of the signals they transmit. Evidence is also accumulating that such conformational specific Nbs do not stabilize new conformational states but rather change the distribution of existing states to allosterically induce transitions, imprinted by the natural ligands of the system.
期刊介绍：
Current Opinion in Structural Biology (COSB) aims to stimulate scientifically grounded, interdisciplinary, multi-scale debate and exchange of ideas. It contains polished, concise and timely reviews and opinions, with particular emphasis on those articles published in the past two years. In addition to describing recent trends, the authors are encouraged to give their subjective opinion of the topics discussed.
In COSB, we help the reader by providing in a systematic manner:
1. The views of experts on current advances in their field in a clear and readable form.
2. Evaluations of the most interesting papers, annotated by experts, from the great wealth of original publications.
[...]
The subject of Structural Biology is divided into twelve themed sections, each of which is reviewed once a year. Each issue contains two sections, and the amount of space devoted to each section is related to its importance.
-Folding and Binding-
Nucleic acids and their protein complexes-
Macromolecular Machines-
Theory and Simulation-
Sequences and Topology-
New constructs and expression of proteins-
Membranes-
Engineering and Design-
Carbohydrate-protein interactions and glycosylation-
Biophysical and molecular biological methods-
Multi-protein assemblies in signalling-
Catalysis and Regulation
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
