Equine bone marrow aspirate and bone marrow aspirate concentrate are enriched with interleukin-1 receptor antagonist protein.

IF 1.3 3区农林科学 Q2 VETERINARY SCIENCES American journal of veterinary research Pub Date : 2025-02-27 DOI:10.2460/ajvr.24.12.0380

Sidney Long, Grigorios Maleas, Zarah A Belacic, Vivian G Quam, Sushmitha Durgam

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Abstract

Objective: To analyze the cellular, growth factor, and cytokine composition of equine sternal bone marrow aspirate (BMA) and laboratory-centrifuged BMA concentrate (BMAC).

Methods: This was an in vitro experimental study. Cellular composition, growth factors (IGF-I, VEGF, PDGF, TGF-β1), and cytokines (IL-6, IL-10, tumor necrosis factor-α (TNF-α), and IL-1 receptor antagonist protein [IL-1Ra]) were quantified using a standard automated hematology analyzer and ELISA methods, respectively, in donor-matched BMA, BMAC (in-house centrifugation protocol), whole blood, and platelet-rich plasma (E-PET; Pall Inc) prepared from 25 horses from 2016 through 2020.

Results: Leukocytes, neutrophils, monocytes, lymphocytes, and thrombocytes were increased 4.1-, 3.4-, 4.6-, 4.4-, and 2.5-fold in BMAC compared to BMA, respectively. Interleukin-1 receptor antagonist protein of BMAC was increased 21-fold compared to BMA and increased 117-fold compared to gravity filtration system-based, leukocyte-rich platelet-rich plasma.

Conclusions: Laboratory centrifugation enriches the cellular and IL-1Ra concentrations of equine BMAC compared to BMA, with no significant changes in IGF-I, VEGF, PDGF, TGF-β1, IL-6, IL-10, or TNF-α concentrations.

Clinical relevance: Equine BMA and BMAC are patient-side biologics enriched with anti-inflammatory IL-1Ra and support further evaluation of equine BMAC for musculoskeletal tissue healing.

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目的：分析马胸骨骨髓抽吸物（BMA）和实验室离心BMA浓缩物（BMAC）的细胞、生长因子和细胞因子组成：分析马胸骨骨髓抽吸物（BMA）和实验室离心BMA浓缩物（BMAC）的细胞、生长因子和细胞因子组成：这是一项体外实验研究。细胞成分、生长因子（IGF-I、VEGF、PDGF、TGF-β1）和细胞因子（IL-6、IL-10、肿瘤坏死因子-α（TNF-α）和IL-1受体拮抗蛋白[IL-1Ra]）分别用标准自动血液分析仪和ELISA方法对供体匹配的BMA、BMAC（内部离心方案）、全血和富血小板血浆（E-PET；Pall Inc）中分别检测供体匹配的 BMA、BMAC（内部离心方案）、全血和富血小板血浆（E-PET；Pall Inc）。结果显示与 BMA 相比，BMAC 中的白细胞、中性粒细胞、单核细胞、淋巴细胞和血小板分别增加了 4.1 倍、3.4 倍、4.6 倍、4.4 倍和 2.5 倍。与 BMA 相比，BMAC 的白细胞介素-1 受体拮抗剂蛋白增加了 21 倍，与基于重力过滤系统的富含白细胞的血小板血浆相比，则增加了 117 倍：结论：与 BMA 相比，实验室离心富集了马 BMAC 的细胞和 IL-1Ra 浓度，但 IGF-I、VEGF、PDGF、TGF-β1、IL-6、IL-10 或 TNF-α 浓度没有显著变化：马BMA和BMAC是富含抗炎IL-1Ra的患者侧生物制剂，支持进一步评估马BMAC在肌肉骨骼组织愈合方面的作用。

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来源期刊

American journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

10.00%

发文量

186

审稿时长

3 months

期刊介绍： The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.