Soil bacterial community composition but not alpha diversity altered along a gradient of Spartina alterniflora invasion on the coast of Yellow Sea, China

Abstract

IntroductionSpartina alterniflora invasion may alter the characteristics and functions of the coastal wetland ecosystems. Previous studies have shown that invasion changes the biogeochemical processes and microbial diversity in marsh ecosystems; however, knowledge of the changes in bacterial communities and their function at different soil depths during invasion remains limited.MethodsIn the present study, we investigated S. alterniflora invasion on the dynamic changes of soil bacterial communities using Illumina MiSeq sequencing analyses of 16S rRNA at different soil depths (i.e., 0–10, 10–20, and 20–40 cm) during the invasive process (i.e., 1, 4, 7, and 12 years), as well as the potential correlations between soil physicochemical characteristics and enzyme activities.ResultsThe invasion of S. alterniflora did not significantly affect soil bacterial alpha diversity or the functional profiles at the first and second levels of the Kyoto Encyclopedia of Genes and Genomes (KEGG). Furthermore, no significant changes were observed across different soil depths. However, the relative abundances of Chloroplast and Alphaproteobacteria increased from 3.03% and 5.05% in bare mudflat to 13.61% and 8.95% in the 12-year-old S. alterniflora invaded soil, respectively, whereas those of Deltaproteobacteria, Acidimicrobiia, and Bacilli decreased after S. alterniflora invasion for approximately 12 years. The relative abundances of soil bacteria varied with soil depth. Total phosphorus, carbon-to-nitrogen ratio, total carbon, and catalase activity were the key factors affecting soil bacterial community composition.DiscussionThe soil physicochemical characteristics changes caused by invasion may temporarily enhance soil fertility, they can lead to long-term wetland degradation through changed biological community and altered nutrient dynamics. This study provides a comprehensive understanding of the dynamic changes in bacterial communities, soil physicochemical characteristics, and enzyme activities during S. alterniflora invasion on coastal wetlands.